BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0150.p (212 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310338|ref|YP_003774430.1| glyoxalase II protein [Herbaspi... 98 6e-22 >gi|300310338|ref|YP_003774430.1| glyoxalase II protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 97.8 bits (242), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 65/194 (33%), Positives = 98/194 (50%), Gaps = 9/194 (4%) Query: 4 IDVSGIDEITYGADDMPACRQFFLDWGMTLAQESAEALVFESQNGCRVIVRNSADPTLPP 63 +++ G D + +G DD+PACRQ+ LD+G+ + A FE+ +G V++R D +LPP Sbjct: 1 MNIIGPDALVFGVDDLPACRQYLLDYGL----KDAGGDRFEALDGTAVVLRAKDDASLPP 56 Query: 64 AIEAGPTLREVVWGVSAAEVLPKFAERI-KDLPGYFNDGKRVGCTDPNGLAVSFQVSQKR 122 A+ LRE V+GV+ + L + +D DG V D G A++FQ++ +R Sbjct: 57 AMGTASLLRETVYGVADSATLDAIETELRRDREVSRRDGV-VRSVDDMGFALAFQLTVRR 115 Query: 123 DVQVECATMNTWNEKNRVNQR--SPVYDHASPIEVGHVVFFVRDVKACEKFYCENFGFVP 180 + + +N R P A P + HVV+FV D E FY GFV Sbjct: 116 PLTLPAERVNGPGHAQRAPNELGLPPELPALPRTLSHVVYFVPDAAKAEAFY-HRLGFVC 174 Query: 181 SDRYPERGAFLRCA 194 +DR+ G FLR A Sbjct: 175 TDRFTGVGPFLRPA 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 674,633 Number of extensions: 25658 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 1 Length of query: 212 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 131 Effective length of database: 1,236,335 Effective search space: 161959885 Effective search space used: 161959885 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)