BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0148.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 182 5e-47 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 165 6e-42 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 143 2e-35 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 143 4e-35 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 142 5e-35 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 182 bits (462), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 89/209 (42%), Positives = 133/209 (63%), Gaps = 6/209 (2%) Query: 1 MVFDDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSIYDSFMERLAAGAAALRVGDPA 60 ++F+DAD+ A G IFS +G+SC+AGSRL V R +YD F+E +AAGA +R+G P Sbjct: 273 IIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVETVAAGAEKIRLGAPL 332 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGYYYAPTIIEGVTN 120 D+ T++GP+ + E I+ V+ G+ G + G TR + GY+ PT++ +N Sbjct: 333 DDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYGEE----GYFVRPTLLAHASN 388 Query: 121 DQRICQEEIFGPVLAAMPFDNEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNV 180 + + EIFGPV A+PF++EE+ IA ANDS + LA +WTRD +A R +V AG Sbjct: 389 AMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVAASVNAGTF 448 Query: 181 WINTYKQFSISTPFGGWRDSGLGPFRTSG 209 W+N+YK ++++PFGG+ SG G R+SG Sbjct: 449 WVNSYKTINVASPFGGFNRSGYG--RSSG 475 Score = 129 bits (325), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 73/188 (38%), Positives = 108/188 (57%) Query: 218 RAIVLYRVAQLIRERGEALAQRQRLDNGKPITETRNLVGSAAATFQFFAAACETLEETIT 277 R LY V +IR E LA+ + + +GKPI + R + A F+++A + + Sbjct: 83 RGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWADKFYGEVI 142 Query: 278 PMRGDNLTMSVYEAMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLMALEL 337 P+ +L + E G V +TPWN+P + ++ PALA GNAV++KP+E+TP +L + Sbjct: 143 PVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSELTPFSSLAI 202 Query: 338 ARICEEAGVPKGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTTTGKHIAHIAADKMMPV 397 AR+ E+AG+P GL++VL G G + VK+V F G TG IA AA ++MP Sbjct: 203 ARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKAAAARVMPC 262 Query: 398 SLELGGKS 405 LELGGKS Sbjct: 263 VLELGGKS 270 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 165 bits (418), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 83/190 (43%), Positives = 117/190 (61%), Gaps = 5/190 (2%) Query: 17 YGIFS-SSGESCIAGSRLFVARSIYDSFMERLAAGAAALRVGDPADERTQMGPLITARHR 75 + +F+ + GE C SR+ V SIY+ F+ER AA++ G+P D+ T +G + Sbjct: 290 FAMFALNQGEVCTCPSRVLVQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQL 349 Query: 76 EGIESYVSIGVADGGRIRTGGTR--LAGDQFARGYYYAPTIIEGVTNDQRICQEEIFGPV 133 E I SY+ IG +G ++ GG R L GD A GYY PT+ +G N RI QEEIFGPV Sbjct: 350 EKILSYIDIGKQEGAKVLAGGGREELGGD-LASGYYVKPTVFQG-NNKMRIFQEEIFGPV 407 Query: 134 LAAMPFDNEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNVWINTYKQFSISTP 193 ++ F +EE+ +A AND++Y L AG+WTRD +A+R GR +QAG VW N Y + Sbjct: 408 VSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAA 467 Query: 194 FGGWRDSGLG 203 FGG++ SG+G Sbjct: 468 FGGYKQSGIG 477 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 82/198 (41%), Positives = 117/198 (59%), Gaps = 2/198 (1%) Query: 210 WAQKKPHERAIVLYRVAQLIRERGEALAQRQRLDNGKPITETRNL-VGSAAATFQFFAAA 268 W + P ERA +L ++A + E LA + LDNGKPI ET + A F++FAAA Sbjct: 72 WGKTSPTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAA 131 Query: 269 CETLEETITPMRGDNLTMSVYEAMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAE 328 T E +I P+ D +E +GVV I PWN PI K+APALAAGN VV+KPAE Sbjct: 132 VRTQEGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAE 191 Query: 329 VTPLMALELARICEEAGVPKGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTTTGKHIAH 388 TP + L + + +P G+++++ G G G + + + +++FTG TTTG+ I Sbjct: 192 QTPASIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQ 250 Query: 389 IAADKMMPVSLELGGKSP 406 A+ ++PV+LELGGKSP Sbjct: 251 YASQNLIPVTLELGGKSP 268 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 80/200 (40%), Positives = 112/200 (56%), Gaps = 4/200 (2%) Query: 1 MVFDDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSIYDSFMERLAAGAAALRVGDPA 60 +VF+DADID A+ GV+ I+ + G+ C AGSRL V I+D F+ RL L+VG P Sbjct: 272 IVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKTRMQKLKVGAPL 331 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGYYYAPTIIEGVTN 120 D+ + MG LI+ E + S V GV +G + ++ D G +Y PT++ GV Sbjct: 332 DKCSDMGALISPVQLERVRSLVEQGVREGAQCH----QVVLDTPPGGCFYPPTLLTGVHP 387 Query: 121 DQRICQEEIFGPVLAAMPFDNEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNV 180 + EEIFGPVL AM F ++ + AN+S Y LAA IW+ A ++AG V Sbjct: 388 AATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALGVAPQLKAGVV 447 Query: 181 WINTYKQFSISTPFGGWRDS 200 WIN+ QF + FGG R+S Sbjct: 448 WINSTNQFDAAVGFGGVRES 467 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 77/198 (38%), Positives = 114/198 (57%), Gaps = 10/198 (5%) Query: 209 GWAQKKPHERAIVLYRVAQLIRERGEALAQRQRLDNGKPITETRNLVGSAAATFQFFAAA 268 GW H RA LY +A+ ++ LA + LDNGKP+ E+R++ A + A Sbjct: 83 GWQALGGHGRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAG 142 Query: 269 CETLEETITPMRGDNLTMSVYEAMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAE 328 L+E+ P D++ + GVV I PWN P+ A K+APALA GN VV+KPAE Sbjct: 143 WAQLQESEFP---DHVPV------GVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAE 193 Query: 329 VTPLMALELARICEEAGVPKGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTTTGKHIAH 388 T L AL A + ++AG+P G+++++ G G+ G A+ HP +++++FTG T G+ I Sbjct: 194 NTSLTALLFAELAQQAGLPAGVLNIVTGDGAT-GAAVVAHPGIQKIAFTGSTEVGRLIRE 252 Query: 389 IAADKMMPVSLELGGKSP 406 A ++LELGGKSP Sbjct: 253 QTAGSGKSLTLELGGKSP 270 Score = 80.5 bits (197), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 53/171 (30%), Positives = 85/171 (49%), Gaps = 4/171 (2%) Query: 210 WAQKKPHERAIVLYRVAQLIRERGEALAQRQRLDNGKPITETRNLVGSAAATFQFFAAAC 269 W PH RA LY +A+ + R E A+R G E R V ++ + A Sbjct: 569 WTSASPHRRAQGLYYIAENLSARTEEFARRIASTTGVTADEARAEVDASIKRLFTYGAWA 628 Query: 270 ETLEETIT--PMRGDNLTMSVYEAMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPA 327 + E + PMRG L M EA+GVV + P P+ + +AP +A GN VVV P+ Sbjct: 629 DKFEGAVHQPPMRGVALAMP--EAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVVVPS 686 Query: 328 EVTPLMALELARICEEAGVPKGLISVLPGKGSVIGDAITLHPLVKRVSFTG 378 E PL+A +L ++ + + +P G+I+++ GK + + + H V+ + G Sbjct: 687 EQAPLVATDLYQVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWVRG 737 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 143 bits (360), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 78/203 (38%), Positives = 113/203 (55%), Gaps = 4/203 (1%) Query: 1 MVFDDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSIYDSFMERLAAGAAALRVGDPA 60 +VFDDA + A+ G G+F + G+ C AGSR+ V RS+ F + LA AA + VGDP+ Sbjct: 263 IVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPS 322 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGYYYAPTIIEGVTN 120 +MGP+ E + Y+ G+ +G + GG + +G++ PTI N Sbjct: 323 QPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGV----SRPDKGWFVQPTIFADANN 378 Query: 121 DQRICQEEIFGPVLAAMPFDNEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNV 180 I +EEIFGPV + FD EE+ IA ANDS Y LAA +WT + +A R V+ G V Sbjct: 379 GMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAV 438 Query: 181 WINTYKQFSISTPFGGWRDSGLG 203 +N + + P+GG +DSG+G Sbjct: 439 GVNCWSPLDANLPWGGIKDSGMG 461 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 78/202 (38%), Positives = 115/202 (56%), Gaps = 5/202 (2%) Query: 210 WAQKKPHERAIVLYRVAQLIRERGEALAQRQRLDNGKPITETRNL-VGSAAATFQFFAAA 268 W++ +R +L+++A L+ + LA G+ E R + V +A + + A Sbjct: 60 WSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGW 119 Query: 269 CETLEETITPMRG----DNLTMSVYEAMGVVAAITPWNSPIASEAQKMAPALAAGNAVVV 324 +E P G L+ +V E +GVV AI PWN+P+ + K+A LAAG VV+ Sbjct: 120 ANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVI 179 Query: 325 KPAEVTPLMALELARICEEAGVPKGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTTTGK 384 KPAE TP AL LAR+ +EAG P G+I+V+ G G+ +G A+ HP V ++SFTG G+ Sbjct: 180 KPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGR 239 Query: 385 HIAHIAADKMMPVSLELGGKSP 406 I IA ++ V+LELGGKSP Sbjct: 240 AIQRIAGEQFKRVTLELGGKSP 261 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 142 bits (359), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 82/198 (41%), Positives = 117/198 (59%), Gaps = 1/198 (0%) Query: 210 WAQKKPHERAIVLYRVAQLIRERGEALAQRQRLDNGKPITETRNLVGSAAATFQFFAAAC 269 W +K ER+ +L R +L+ + LA + GKP+ E R +G AA+ ++FA Sbjct: 65 WKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEG 124 Query: 270 E-TLEETITPMRGDNLTMSVYEAMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAE 328 + T +TI N + + EA+GV AAITPWN P A +K PALAAG +V+KPAE Sbjct: 125 KRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAE 184 Query: 329 VTPLMALELARICEEAGVPKGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTTTGKHIAH 388 TP AL LA + E AG+P G+ SV+ G IG +T +P+V+++SFTG T GK + Sbjct: 185 ATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQ 244 Query: 389 IAADKMMPVSLELGGKSP 406 +A + +SLELGG +P Sbjct: 245 QSASSIKKLSLELGGNAP 262 Score = 139 bits (351), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 70/204 (34%), Positives = 117/204 (57%), Gaps = 6/204 (2%) Query: 1 MVFDDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSIYDSFMERLAAGAAALRVGDPA 60 +VFDDAD+D AV G + + ++G++C+ +R++V +YD+F +L +VG Sbjct: 264 IVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGT 323 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGY-YYAPTIIEGVT 119 +E GPLI + + +E +V+ VA G R+ GG R A G+ ++ PT++ VT Sbjct: 324 EEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKR-----HALGHSFFEPTVLADVT 378 Query: 120 NDQRICQEEIFGPVLAAMPFDNEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGN 179 ++ +EE FGP+ F +E+++A AND+ + LA+ ++RD + WR +++G Sbjct: 379 PAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGM 438 Query: 180 VWINTYKQFSISTPFGGWRDSGLG 203 V INT + PFGG + SGLG Sbjct: 439 VGINTGLISNEVAPFGGVKQSGLG 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,440,863 Number of extensions: 60482 Number of successful extensions: 286 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 242 Number of HSP's successfully gapped: 38 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)