BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0012.0146.p
         (657 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I...    86   1e-17
gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi...    74   5e-14

>gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III
           large subunit [Herbaspirillum seropedicae SmR1]
          Length = 572

 Score = 85.9 bits (211), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 96/419 (22%), Positives = 177/419 (42%), Gaps = 37/419 (8%)

Query: 1   MVEALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQLSMLKAISKAAFRIRSTETAISTI 60
           +  A     P++ ++GQ+ +  + Q      +  D + + +   K  F ++  +    T+
Sbjct: 88  LATAYMDSIPMVVISGQVPSTAIGQDA---FQECDTVGITRPCVKHNFLVKDVKDLAETV 144

Query: 61  KLAVQTALTPPMGPVSVEIPIDIQAALIPMPSDLRPLPIAVQAPSEHALDE--------L 112
           K A   A T   GPV V+IP DI      M +     P AV+  S   +D+         
Sbjct: 145 KKAFYIATTGRPGPVLVDIPKDIT-----MHTCAYAYPKAVEMRSYKPVDKGHSGQIRKA 199

Query: 113 AERLLKAGRPLLWLGGGA--RRAGKQVQRLLD-LGFGVVTSTQGRGIVAEDDPRSLGSFN 169
            + LL A RP+++ GGG     A  ++ +L+D LG+    +  G G       + +G   
Sbjct: 200 LQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGAYRSSSDKFVGMPG 259

Query: 170 LHKPVE-NF-YQTCDAIVVIGSRLR----GNETLKYELKLPRPLYRIDADPSAEGRCYNS 223
           +H   E N   Q CD ++ IG+R      GN   K+     R +  ID DPS+  +    
Sbjct: 260 MHGTYEANMAMQHCDVLIAIGARFDDRVIGNP--KHFASHARKIIHIDIDPSSISKRVKV 317

Query: 224 DHFVCGDAALALEGLADRL---EKRKSAIHIDPAF--MADLKKARDAAVAGLVDGLGPYS 278
           D  + G+    L+ L  +L   E R++   +D  +  + + +K      A   + + P S
Sbjct: 318 DIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRDCLKFATSEEFIKPQS 377

Query: 279 ALVEQVQKAAGRKFNWVRDVTVSNSTWGNRYLRIFDPLAGVHALGGGIGQGLAMGIGAAI 338
            + +  +   G  F  +      +  W  +Y     P   +++  GG+G  + +G+  A+
Sbjct: 378 VVQKVWEVTKGDAF--ITSDVGQHQMWAAQYYGFDKPRRWINS--GGLGT-MGVGLPYAM 432

Query: 339 GAAVTASGKKTFTLAGDGGFILNLGELATAVQEKADMVIVLMNDKGYGVIKNIQDVQRG 397
           G  +         + G+    + + ELAT  Q      I+L+N++  G+++  Q +  G
Sbjct: 433 GVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQQIDYG 491


>gi|300311743|ref|YP_003775835.1| acetolactate synthase
           [Herbaspirillum seropedicae SmR1]
          Length = 625

 Score = 73.6 bits (179), Expect = 5e-14,   Method: Compositional matrix adjust.
 Identities = 92/381 (24%), Positives = 166/381 (43%), Gaps = 45/381 (11%)

Query: 40  LKAISKAAFRIRSTETAISTIKLAVQTALTPP--MGPVSVEIPIDIQAALIPMPSDL--- 94
            + +S+   RI   E  ++ +  A+Q  LT P   GPV++ +P D+Q      P D    
Sbjct: 153 FRPLSRMFDRICYPEQLLTALPRAIQV-LTDPAQCGPVTLALPQDVQTMAYDYPLDFFSP 211

Query: 95  RPLPIAVQAPSEHALDELAERLLKAGRPLLWLGGGAR--RAGKQVQRLLDL-GFGVVTST 151
           R +    QAP+   L++    L +A +PLL  GGG     A + ++R  +  G  V  + 
Sbjct: 212 RVIVPRAQAPAAQELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVPVAETQ 271

Query: 152 QGRGIVAEDDPRSLGSFNLH-KPVEN-FYQTCDAIVVIGSRLR----GNETLKYELKLPR 205
            G+  +       +G+  +   P  N   Q  D ++ +G+RL+    G+ TL  + +L  
Sbjct: 272 AGKSALPWSHALQMGAIGVTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQARLLN 331

Query: 206 PLYRIDADPSAEGRCYNSDHFVCGDAALALEGLADRLEKRKSA--IHIDPAFMADLKKAR 263
               ++A  + + R       +  DA L LE L+  LE  +SA   H     +A   + R
Sbjct: 332 --LNVNAMDAHKWRGLA----LQADAGLGLEALSHALEGWRSAPEWHARAHTLAQDWRER 385

Query: 264 DAAVAGLVDGLG--PYSA-LVEQVQKAAGRKFNWVRDVTVS---------NSTWGNRYLR 311
            A + G  D  G  PY   ++  +Q++     +  +D+ V          +  W      
Sbjct: 386 VAQITGQTDTGGRLPYDGEVIGAIQRSVADSPS--QDIVVCAAGTLPAELHKLWRAGRPG 443

Query: 312 IFDPLAGVHALGGGIGQGLAMGIGAAIGAAVTASGKKTFTLAGDGGFILNLGELATAVQE 371
            +    G   +G  +  GL        G  +    ++   + GDG +++   ELAT+V  
Sbjct: 444 AYHVEYGYSCMGYEVAGGL--------GVKLAQPQREVIVIVGDGSYLMMNSELATSVML 495

Query: 372 KADMVIVLMNDKGYGVIKNIQ 392
            A +++V+++++GYG I  +Q
Sbjct: 496 GAKLIVVVLDNRGYGCINRLQ 516


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,651,659
Number of extensions: 60830
Number of successful extensions: 226
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 226
Number of HSP's successfully gapped: 2
Length of query: 657
Length of database: 1,619,870
Length adjustment: 91
Effective length of query: 566
Effective length of database: 1,188,985
Effective search space: 672965510
Effective search space used: 672965510
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 134 (56.2 bits)