BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0145.p (128 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 193 5e-51 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 146 5e-37 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 134 2e-33 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 74 3e-15 gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 69 8e-14 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 193 bits (490), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 92/98 (93%), Positives = 96/98 (97%) Query: 1 MIDFALEAERHEGKSPRDAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLG 60 MIDFALEAERHEGKSPR+AIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLG Sbjct: 930 MIDFALEAERHEGKSPREAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLG 989 Query: 61 ITMVGGLLVSQVLTLFTTPVIYLGFDSLAKRMRKRFGK 98 ITMVGGLLVSQVLTLFTTPVIYLGFDSL +R+R RFG+ Sbjct: 990 ITMVGGLLVSQVLTLFTTPVIYLGFDSLGRRLRARFGQ 1027 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 146 bits (368), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 65/101 (64%), Positives = 87/101 (86%) Query: 1 MIDFALEAERHEGKSPRDAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLG 60 M+DFA++AER+ G SPRDAI++AC LRFRPI+MTTMAALLGA+PL +G G G+ELRQPLG Sbjct: 972 MVDFAIDAERNRGMSPRDAIFEACKLRFRPIMMTTMAALLGAIPLAIGHGDGAELRQPLG 1031 Query: 61 ITMVGGLLVSQVLTLFTTPVIYLGFDSLAKRMRKRFGKEKH 101 I++VGGL+VSQ+LTL+TTPV+YL + L+ ++RFG++ Sbjct: 1032 ISIVGGLVVSQLLTLYTTPVVYLYMERLSSWSKRRFGRKNK 1072 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 134 bits (337), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 67/100 (67%), Positives = 77/100 (77%), Gaps = 1/100 (1%) Query: 1 MIDFALEAERHEGKSPRDAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLG 60 MIDFAL AER +G P AIYQACLLRFRPI+MTTM ALL LPLMLG G GSELR+PLG Sbjct: 940 MIDFALTAERSQGMKPEQAIYQACLLRFRPIMMTTMCALLSGLPLMLGHGAGSELRRPLG 999 Query: 61 ITMVGGLLVSQVLTLFTTPVIYLGFD-SLAKRMRKRFGKE 99 MVGGL++SQ LTLFTTPV+YL D + MRK+ ++ Sbjct: 1000 YAMVGGLILSQALTLFTTPVVYLYLDRAHYWYMRKKEARQ 1039 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 74.3 bits (181), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 41/108 (37%), Positives = 63/108 (58%), Gaps = 2/108 (1%) Query: 1 MIDFALEAERHEGKSPRDAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLG 60 ++ FA + R EG +P A +A R RP+LMT +A ++G +P+ LG G G+E PLG Sbjct: 957 IVAFARQ-RREEGATPLAAALEAGSTRIRPVLMTALAMIIGMIPMALGLGEGAEQNAPLG 1015 Query: 61 ITMVGGLLVSQVLTLFTTPVIYLGF-DSLAKRMRKRFGKEKHPGHPDD 107 ++GGL+++ V TLF PV++ G LA+R +R P H + Sbjct: 1016 RAVIGGLMLATVSTLFFVPVVFAGVHQRLARRADQRRALAGGPAHQES 1063 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 69.3 bits (168), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 35/91 (38%), Positives = 58/91 (63%), Gaps = 1/91 (1%) Query: 1 MIDFALEAERHEGKSPRDAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLG 60 +++FA E E +G++ A +A LR RPILMT++A ++G +PL+ +G GSE+R +G Sbjct: 953 IVEFARELE-MQGRTAVQAAIEASRLRLRPILMTSIAFIMGVVPLVTSSGAGSEMRHAMG 1011 Query: 61 ITMVGGLLVSQVLTLFTTPVIYLGFDSLAKR 91 I + G+L + LF TPV Y+ ++ +R Sbjct: 1012 IAVFFGMLGVTLFGLFLTPVFYVLLRTIDRR 1042 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 263,868 Number of extensions: 6917 Number of successful extensions: 50 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 51 Number of HSP's successfully gapped: 10 Length of query: 128 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 53 Effective length of database: 1,264,745 Effective search space: 67031485 Effective search space used: 67031485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)