BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0143.p (123 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 135 1e-33 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 105 2e-24 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 101 1e-23 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 67 5e-13 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 67 5e-13 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 135 bits (339), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 66/87 (75%), Positives = 80/87 (91%) Query: 1 VLVILNRQPGANIIETVDAVNGLLPNLKASIPHAINLEVMMDRTPTIRASLKDTEKTLLM 60 VL+I+NRQPGANII+TVD V LLP L+ASIP AI+++V++DRTPTIRASLK+TE TL++ Sbjct: 281 VLIIINRQPGANIIDTVDNVRELLPQLQASIPSAIDMKVVLDRTPTIRASLKETEFTLIL 340 Query: 61 SIALVIMVVFLFLRNGRATLIPSVAVP 87 S+ALVIMVVFLFLRN RATLIP+VAVP Sbjct: 341 SVALVIMVVFLFLRNARATLIPAVAVP 367 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 105 bits (261), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 50/87 (57%), Positives = 70/87 (80%) Query: 1 VLVILNRQPGANIIETVDAVNGLLPNLKASIPHAINLEVMMDRTPTIRASLKDTEKTLLM 60 V+V + RQPGAN+I VD++ +LP L+AS+P AI++ ++ DRT TIRAS+ D + LL+ Sbjct: 281 VIVNIQRQPGANVIAVVDSIKKILPQLQASLPGAIDVHILTDRTTTIRASVDDVKFELLL 340 Query: 61 SIALVIMVVFLFLRNGRATLIPSVAVP 87 S+ALV+MV+F+FLRN AT+IPSVAVP Sbjct: 341 SVALVVMVIFVFLRNVPATIIPSVAVP 367 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 101 bits (252), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 48/87 (55%), Positives = 70/87 (80%) Query: 1 VLVILNRQPGANIIETVDAVNGLLPNLKASIPHAINLEVMMDRTPTIRASLKDTEKTLLM 60 +++ + +QPGAN+IETV + +P L+A+IP AI++ ++DRT TIRAS++D E TL++ Sbjct: 293 IVLAITKQPGANVIETVGRIKAAMPRLRAAIPAAIHVNTLIDRTQTIRASVEDVEFTLVL 352 Query: 61 SIALVIMVVFLFLRNGRATLIPSVAVP 87 +IALV+MV+F+FLRN ATLIPSV VP Sbjct: 353 TIALVVMVIFVFLRNVAATLIPSVTVP 379 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 67.0 bits (162), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 34/80 (42%), Positives = 53/80 (66%), Gaps = 2/80 (2%) Query: 10 GANIIETVDAVNGLLPNLKASIPHAINLE--VMMDRTPTIRASLKDTEKTLLMSIALVIM 67 GAN + T DAV L L+ P ++ L+ + D TP +R SL++ KTL+ +I LV++ Sbjct: 296 GANALATADAVKKKLAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVL 355 Query: 68 VVFLFLRNGRATLIPSVAVP 87 +++LFL+N RAT IP++ VP Sbjct: 356 IMYLFLQNIRATFIPAITVP 375 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 66.6 bits (161), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 31/78 (39%), Positives = 54/78 (69%) Query: 10 GANIIETVDAVNGLLPNLKASIPHAINLEVMMDRTPTIRASLKDTEKTLLMSIALVIMVV 69 GAN ++TV A++ + L+ P + ++ D TP +R S+++ +TL+ ++ LV +V+ Sbjct: 296 GANALDTVKAIDKRVAELEKFFPTGMKVQKPYDTTPFVRISIEEVVRTLIEAVVLVFLVM 355 Query: 70 FLFLRNGRATLIPSVAVP 87 +LFL+N RATLIP++AVP Sbjct: 356 YLFLQNFRATLIPTIAVP 373 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 266,130 Number of extensions: 7903 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 32 Number of HSP's successfully gapped: 8 Length of query: 123 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 49 Effective length of database: 1,269,480 Effective search space: 62204520 Effective search space used: 62204520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)