BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0136.p (472 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 724 0.0 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 520 e-149 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 352 5e-98 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 239 5e-64 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 136 4e-33 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 724 bits (1869), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/444 (80%), Positives = 400/444 (90%) Query: 22 MDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISGWMADRFGALTIFRSAIC 81 MDSTVISTSLP LA+DM VDPISLKLALTSYLVSLAVFIPISGWMADRFG+LTIFRSAIC Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 82 VFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANLVKALSYLTIPA 141 VFMLGSILCGVS +LP+FVAARF QGIGGAMMVPVGRLVILRSV+K NLVKALSYLTIPA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 142 MFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANAKSESVAPLDWVGFVL 201 +FGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFI + K+E VAPLD GF+L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 202 SALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAYVWHARRTEHPLIDLKLLRIET 261 SALGCS++MMGLAT GRHLVSG+++L CV+ G + LAAYVWHARR PLIDL LL+I T Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 262 LQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASAAGAMFMKTIAAKFLK 321 L+AGV GGS +R+GIGAIPFLLPLMLQLGFG++ FHSG+LTCASAAGAMFMKTIAA+FL+ Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 322 RYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLIGGCFRSLQFTSLNAISYADV 381 RYGFRRV++VNGA GV+ L AI FTP TPY +M+++LLIGGCFRSLQFTSLNAISYADV Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 382 DNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGHATLVSQDFWPAFLTVAVAAS 441 D RLSQATS+ASVAQQLS+GMGVVL GFALQMSN IQGHATLV+QDFWPAFL V A+ Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 442 FSIPLLMRLPPDAGAELAGRNQAK 465 ++ +L +LP DAGAE+AGR+ + Sbjct: 421 CALLMLRQLPHDAGAEMAGRDDNR 444 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 520 bits (1339), Expect = e-149, Method: Compositional matrix adjust. Identities = 253/438 (57%), Positives = 337/438 (76%) Query: 22 MDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISGWMADRFGALTIFRSAIC 81 MD+TV++TSLP LA D+ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ GA +F +A+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 82 VFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANLVKALSYLTIPA 141 VFM GSILC VS SL FVAARF QGIGGAMMVPVGR++I RSVDK+ LVKA+SYLT+PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 142 MFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANAKSESVAPLDWVGFVL 201 + GPV+GP LGGFITTYFHWRW+F INIP LGLYLA RFI N K ++ APLD GFVL Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 202 SALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAYVWHARRTEHPLIDLKLLRIET 261 +++G +M+M+GL+ G HLV G +A +G+++L Y H+ + E PL+D +LLRI T Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 262 LQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASAAGAMFMKTIAAKFLK 321 L A V GGSLFR+G+GA+PFLLPL LQ G G +AF +G +TCASA G++F++ +A + L+ Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 322 RYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLIGGCFRSLQFTSLNAISYADV 381 RYGFR V++VN ++LAA G F+ TPY +++ ++L+GG F +LQFT LN+I+YAD+ Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 382 DNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGHATLVSQDFWPAFLTVAVAAS 441 D+ + TS+ASV QQLS+G+GV + G LQ+SNH+QGHAT+V DFWPAFL + + + Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 442 FSIPLLMRLPPDAGAELA 459 S+P+ +LP +AG EL+ Sbjct: 421 ASVPISGKLPHNAGLELS 438 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 352 bits (902), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 204/471 (43%), Positives = 287/471 (60%), Gaps = 8/471 (1%) Query: 5 RTFTIPLIVACALFMEQMDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISG 64 R +P IVA FME +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISG Sbjct: 28 RVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISG 87 Query: 65 WMADRFGALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRS 124 W+ADRFG+ T+F AI +F L SILCG SQ++ EF AAR QG+GGAMMVPVGR++++RS Sbjct: 88 WVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRS 147 Query: 125 VDKANLVKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIA 184 K L+KA++ +T PA+ PV+GP +GGFITTY W WIF +N+P + + Sbjct: 148 TGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVR 207 Query: 185 NAKSESVAPLDWVGFVLSALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAYVW-H 243 N S PLD VGF+LS + ++ G + ++L V LG + L W H Sbjct: 208 NEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALG-VVLGYGAWRH 266 Query: 244 ARRTEHPLIDLKLLRIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTC 303 +R EHPL+D LRI T V GSL R+G+ A+P+L+PLM Q+GFG +AF SGLL Sbjct: 267 MQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLL 326 Query: 304 ASAAGAMFMKTIAAKFLKRYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLIGG 363 A+A G + MK + L+R+GFR V + N A + A G TPATP V+L V+ G Sbjct: 327 ATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYG 386 Query: 364 CFRSLQFTSLNAISYADVDNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGHAT 423 RS+QF++L ++YAD+ + R S A+++ SVAQQ+++GMG+ G AL+++ ++G A Sbjct: 387 LSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEAI 446 Query: 424 LVS------QDFWPAFLTVAVAASFSIPLLMRLPPDAGAELAGRNQAKAGS 468 S DF AF V S+ L DAG+ + G +A G Sbjct: 447 DASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNIGGGMRAAPGK 497 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 239 bits (609), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 143/418 (34%), Positives = 230/418 (55%), Gaps = 7/418 (1%) Query: 12 IVACALFMEQMDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISGWMADRFG 71 IVA FM+ +D+T+++T+LP +A + +++ + +Y +++A P SGW+ADRFG Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 72 ALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANLV 131 ++ +AI +F LGS+LC ++ SLP +AAR QG+GG+M++PVGRL +LR+V A + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 132 KALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANAKSESV 191 AL+ ++I GPV+GP LGG++ W WIF IN+P+ +LG+ F+ + Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 192 APLDWVGFVLSALGCSMVMMGLATT---GRHLVSGDISLACVVLGSLSLAAYVWHARRTE 248 D +G VL L MV LA H I+L V+ +L++ Y HA Sbjct: 237 PRFDLLGCVL--LSACMVAFSLALDVPFDSHRTGWSIAL--FVVAALTVLGYWRHASVHP 292 Query: 249 HPLIDLKLLRIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASAAG 308 PL L L G+ G + R+G A+PFLLPL+LQ+ G+S H+G++ +A Sbjct: 293 RPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIA 352 Query: 309 AMFMKTIAAKFLKRYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLIGGCFRSL 368 K A +KR+G+ R ++VN A ++ + TP P ++ L I G S+ Sbjct: 353 GTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSM 412 Query: 369 QFTSLNAISYADVDNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGHATLVS 426 QF ++N+++ +D S+ S+ Q L++G+GV +GG + + G AT S Sbjct: 413 QFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTATNAS 470 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 136 bits (342), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 100/329 (30%), Positives = 171/329 (51%), Gaps = 20/329 (6%) Query: 11 LIVACALFMEQMDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISGWMADRF 70 L V+ A+FM +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 71 GALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANL 130 GA+ +F +++ +F+L S LCG++ +LP +AAR QG+ MVP+ + ++L + K+ Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKS 146 Query: 131 VKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISIL--GLYLATRFIANAKS 188 AL+ + A+ PV GP LGG+IT + W WIF+INIP+ ++ G+ LA + Sbjct: 147 SFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPT 206 Query: 189 ESVAPLDWVGFVLSALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAY----VWHA 244 + P+D +G L + + L G+ L S V LG ++L A+ VW Sbjct: 207 HKL-PIDKIGLALLVTWVAAFQIML-DKGKDL-DWFASPTIVTLGIIALIAFAYFVVWEL 263 Query: 245 RRTEHPLIDLKLLRIETLQAG-----VGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSG 299 +HP++DL+L G +G G F G + +LP LQ G+ + SG Sbjct: 264 NN-DHPVVDLRLFGRRNFAGGTIAISIGYGVFF----GNL-VILPQWLQQYLGYRSIDSG 317 Query: 300 LLTCASAAGAMFMKTIAAKFLKRYGFRRV 328 L T A+ + + +FL R+V Sbjct: 318 LSTAPIGIFAVILMPLIGRFLPLVDARKV 346 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.140 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,360,802 Number of extensions: 55324 Number of successful extensions: 354 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 332 Number of HSP's successfully gapped: 16 Length of query: 472 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 383 Effective length of database: 1,198,455 Effective search space: 459008265 Effective search space used: 459008265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)