BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0012.0134.p
         (326 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310858|ref|YP_003774950.1| magnesium and cobalt transport ...   514   e-147
gi|300309744|ref|YP_003773836.1| magnesium and cobalt transport ...    67   2e-12

>gi|300310858|ref|YP_003774950.1| magnesium and cobalt transport
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 328

 Score =  514 bits (1325), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 244/328 (74%), Positives = 281/328 (85%), Gaps = 2/328 (0%)

Query: 1   MNNANMVVNCTAYKNGKKLRDITIEEISDVLLEENTFVWVGLLEPSVELMRQIKEEFNLH 60
           M +  M+VNCT YK G+KLR+I+++EISDVL EE TFVW+GLLEP+VELM +IKEEF LH
Sbjct: 1   MIDPEMIVNCTVYKKGRKLREISLDEISDVLEEEGTFVWLGLLEPNVELMNKIKEEFCLH 60

Query: 61  ELAVEDAYAAHQRPKIEEYGDTLFVVLQTAQVVDAVVQFGETHIFVGARFVVSVRHGSSK 120
           ELAVEDAYAAHQRPKIEEYGDTLFVVL TAQ+    VQFGETHI  G RFVV+VRHG+S+
Sbjct: 61  ELAVEDAYAAHQRPKIEEYGDTLFVVLHTAQLTGGEVQFGETHILTGPRFVVTVRHGASR 120

Query: 121 SYAKVREHCEMLPERLVRGPSYVLYSIMDFVVDNYGPIMDDLEERFDKYEAELFD--RVS 178
           SYAKVREHCEMLPERLVRGPS+VLYSIMDFVVDNY PIMDDLE +F +YE +LF+   +S
Sbjct: 121 SYAKVREHCEMLPERLVRGPSFVLYSIMDFVVDNYRPIMDDLEAKFSQYEEQLFNPGTIS 180

Query: 179 EQAHLQDLYQLKRQLIKIRSAAVPLLDVCTQLLRFHENIITKDGKVYFRDIYDHVVRLIE 238
            +A+LQ+LYQLKRQLI++RSAA PLLDVCTQLLRFHE +ITKDGK YFRDIYDHVVR I+
Sbjct: 181 GKAYLQELYQLKRQLIRLRSAASPLLDVCTQLLRFHEGLITKDGKAYFRDIYDHVVRFID 240

Query: 239 TGDRLREMVAAAMQIELAQVTIRQNEVVKRLAGWGGILAIPTMMFSLYGMNFKYMPELEW 298
           TGDRLREMV +A+QI LAQVTI QNE+V+ LAGWG ILAIPTM+FSLYGMNF +MP L W
Sbjct: 241 TGDRLREMVESAVQINLAQVTIHQNEIVQTLAGWGAILAIPTMIFSLYGMNFAHMPGLSW 300

Query: 299 HWGYPTVLIAIGGSCSWLYYRLRKVGWL 326
            W +P VL  I  +C+ L+ RLRK GWL
Sbjct: 301 EWSFPLVLGGIVSACTLLWRRLRKAGWL 328


>gi|300309744|ref|YP_003773836.1| magnesium and cobalt transport
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 321

 Score = 66.6 bits (161), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 91/360 (25%), Positives = 151/360 (41%), Gaps = 80/360 (22%)

Query: 7   VVNCTAYKNGKKLRDITIEEISDVLLEENTFVWVGLLEPSVELMRQIKEEFNLHELAVED 66
           ++N    +NG+ L  + I+  +D  LE+   VWV L EP+ E    +K  + +       
Sbjct: 1   MINVFVLQNGR-LSQVNIDSRTD--LEQAAPVWVDLTEPNDEERAWVKSIYGV------- 50

Query: 67  AYAAHQRPKIEEYGDTLFVVLQTAQVVDAVVQFGETHIFVGARFVVSVRHGSSKSYAKVR 126
                  P  +E+ D    +  +A+  +A  + G+ H+     F       S+ + A + 
Sbjct: 51  -----TLPDEDEFSD----IEASARYFEA--ENGDLHLRTDFLFEGDDDSSSTMTVAFIL 99

Query: 127 E-------HCEMLPE-RLVR----GPSYVLYSIMDFVVDNYGP----IMDDLEERFDKYE 170
                   H E LP  RLVR         +    D ++D Y        D LE  + K E
Sbjct: 100 ARNILFSVHAEDLPVFRLVRMRARSRPGSIGDYRDVLLDLYETDAEYSADALEGIYQKLE 159

Query: 171 AE----LFDRVSEQ---------AHLQDLY-QLKRQLIKIRSAAVPLL----------DV 206
           A     L   +S+Q         AH +DL  +++R ++  R A   L+          + 
Sbjct: 160 AVSTSVLKKSLSDQDAAEVLNTIAHEEDLNGRIRRNMMDTRRAVSFLMRGRLLSSEQFED 219

Query: 207 CTQLLRFHENIITKDGKVYFRDIYDHVVRLIETGDRLREMVAAAMQIELAQVTIRQNEVV 266
             Q+LR   +I + DG   F  ++D +  L              M   +  + I QN+++
Sbjct: 220 ARQILR---DIESLDGHTAF--LFDKINFL--------------MDATVGFININQNKII 260

Query: 267 KRLAGWGGILAIPTMMFSLYGMNFKYMPELEWHWGYPTVLIAIGGSCSWLYYRLRKVGWL 326
           K  +        PT++ S+YGMNFK MPEL+W  GYP  ++ + GS    ++  R+ GWL
Sbjct: 261 KIFSVASVAFLPPTLIASIYGMNFKIMPELDWAIGYPLAILMMIGSAIAPFWYFRRRGWL 320


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.139    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 979,308
Number of extensions: 39160
Number of successful extensions: 152
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 152
Number of HSP's successfully gapped: 2
Length of query: 326
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 241
Effective length of database: 1,217,395
Effective search space: 293392195
Effective search space used: 293392195
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 131 (55.1 bits)