BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0133.p (1348 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310866|ref|YP_003774958.1| signal peptide protein [Herbasp... 1519 0.0 >gi|300310866|ref|YP_003774958.1| signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 1300 Score = 1519 bits (3932), Expect = 0.0, Method: Compositional matrix adjust. Identities = 783/1296 (60%), Positives = 988/1296 (76%), Gaps = 31/1296 (2%) Query: 51 TDSGTRSAWRLAVWSMQGKLSGVHGGGSLADGVHLRNLRYRDAAMQLDIDRIDAAWDLSL 110 T+SG R W AV ++ GKLSG GG++ADG+HLR + Y+D LDIDR+DA+W LSL Sbjct: 15 TESGARLLWNTAVKTLGGKLSGQLLGGTVADGLHLRAVHYQDGQRILDIDRLDASWRLSL 74 Query: 111 RQRRLTVSYLRVGTVDINKQPLPPTPTIMPSSLSLPLALNLNDVSLQKLILQQGTSVTEL 170 +RR +++YLR GTV +N+QP P P+ +P+SLSLPLAL + ++SL K L QGTS TEL Sbjct: 75 LKRRFSLAYLRAGTVQLNQQPSPSEPSSLPASLSLPLALEIGEISLDKFSLTQGTSTTEL 134 Query: 171 SGLQLHGTSDGVQHTLVLDKLTTAFGQATALLHLNGKQPFALNGGVELAGLYQQDQQDQQ 230 S L+LHG+SDGVQHTLVLD L T +GQATAL+HLNGK PF + GG+EL G+YQ + Sbjct: 135 SKLRLHGSSDGVQHTLVLDSLVTPYGQATALVHLNGKAPFDIGGGLELGGVYQHGE---- 190 Query: 231 EKSSEKFQLAAQLSGSLQELGIALTATGDKLNGKADIVATPFSTVPLKRAKVDLQHVNPQ 290 E++QLAAQLSGSLQELG+AL A+GDKL G ADI+ATPF+ VPLKRA+VDLQH+NPQ Sbjct: 191 --FKEQYQLAAQLSGSLQELGVALKASGDKLKGDADIIATPFAAVPLKRARVDLQHLNPQ 248 Query: 291 AFSNGAPKADLQ-----RPVAGATAEN-LRVAGPVDIVNAIPGSLDQQRLPLTSIKARVD 344 AF AP ADL P AG A+ L+VAG ++I NA PGSLDQQ LPL S A + Sbjct: 249 AFHATAPAADLSVRADLAPSAGQAADAPLKVAGRIEINNARPGSLDQQLLPLLSASADLQ 308 Query: 345 LGVDSQQLDDLRIALLKNATLTGKGQFHPADK--------AGRFDFDVAGLDLQAIHSEL 396 L + Q L L I L +N + G GQF P + AGR V+ LDL IH ++ Sbjct: 309 LDLQQQALTRLEIRLPRNGRIEGAGQFQPGSQDPAQPTPMAGRLALRVSELDLSQIHHQV 368 Query: 397 KPTRLAGPLNVVLKPGGQDIMLKLQDAQYSIQANAAVTPEQVTLKQVLLTAQKARLELAG 456 +PTRL+GP+ L P + ++L LQD+ + ++ + + V L++ LTA K+RLE AG Sbjct: 369 RPTRLSGPVEATLGPDARQLLLTLQDSALRLHLDSTLRADTVELREASLTAGKSRLEAAG 428 Query: 457 TLGTVGAMEYAFKGKLANFDPSLWMKTGKAPANAKA---GTAAHAISARINMDFDTAGSL 513 ++ TVGAM+YAFKG+L +FDP+ ++T AP A A G AI ARIN+DF+ AG L Sbjct: 429 SMKTVGAMDYAFKGRLVDFDPTAVLQTVTAPTAASARRGGKTVAAIPARINLDFNAAGLL 488 Query: 514 SPELQARLNFIIHDSSYDQLPMSGSGKLQLAGKRLLPSSLDMLVAGNQLQLKGAFGAPSD 573 SPEL+ +L F IHDSSY LPM+G G + LAG RLLPS + VAGN L L G+FGA +D Sbjct: 489 SPELRLKLGFTIHDSSYGDLPMAGRGDIDLAGMRLLPSQAHLQVAGNTLDLNGSFGAAND 548 Query: 574 KLDIHIDAPQLARLGFGVAGMLKLDGQLTGSMQRPNLRATYSGRQLAFGPHHLNRVDGQA 633 KL + IDAPQLARLG+G++G+L+LDGQL G+ QRP+L+A+Y QL FGPH L + GQA Sbjct: 549 KLKLKIDAPQLARLGYGLSGLLRLDGQLGGTRQRPSLQASYQAEQLGFGPHKLQHLAGQA 608 Query: 634 DLQTDLNASAASASNKLQLSIDGDGYSGPDAALKQLKLSLSGTFGEHKLAVQADGRVRNQ 693 ++ ++L A AS N+L L+++GDGYSGP A L QL + L GT+ +H L+++A+G++ N+ Sbjct: 609 EIHSNLAAGVASPENRLALTLEGDGYSGPQAELAQLAVKLDGTYAKHSLSLKANGKLLNR 668 Query: 694 TLALQLNAHGKVTQDKNG-YGWNGVIDKLDNQGLPRIALAAPLSLALAADTLVAGATRLN 752 LAL L AHG+VT+ G YGWNG ID+L N G PRIA+ PL+L + + + AG+TR+ Sbjct: 669 PLALTLAAHGQVTEKTRGQYGWNGSIDQLSNDGFPRIAMLHPLALQASPEAVEAGSTRIE 728 Query: 753 VDRMAIDLRSLSYQQGRIRSEGSAKTLDVGRILELVREMSGASAAAPPAKTDLVLDADWN 812 +DR A+DLR LS++QGRI S G + LDVGR+LEL +++ AAAPP KTDLVLD+DW+ Sbjct: 729 IDRTALDLRHLSWRQGRIASAGEVRALDVGRLLELAQQLG---AAAPPMKTDLVLDSDWD 785 Query: 813 FSLAETVSGFFQVLRKSGDVSVNTGASQVALGLSALQLRVDLAGHEAKFNGGVTASRIGT 872 FSLAE+ G+ Q+ R+SGD+ + T + LGLS L+LR D G + + +G V ASRIGT Sbjct: 786 FSLAESARGYVQIARQSGDLRLVTSNGEATLGLSELKLRSDFQGEQMRLSGRVDASRIGT 845 Query: 873 IEAQGQAGLLRQDGVLTLLPDSPLTAHARLNVPEVKTIGALLGPQYSLDGKLAMDINAAG 932 + GQ GL R D +LTL SPL +ARL +PE+K IG L+GPQ SL+GKL+ D+N AG Sbjct: 846 LALGGQIGLQRVDQLLTLADGSPLALNARLELPELKRIGDLIGPQVSLNGKLSADLNVAG 905 Query: 933 TLAKPRLSGAINGDSLAVTLFDQGIQLKDGTVRIVMDDNVIDLRQIEFHGGEGTLRATGK 992 TLA+P+LSGA+NGD LAVT FDQGIQLKDG VRIVMD NVIDLRQIEFHGG GTLRA GK Sbjct: 906 TLAQPKLSGAVNGDDLAVTQFDQGIQLKDGIVRIVMDSNVIDLRQIEFHGGRGTLRAGGK 965 Query: 993 VKLGDADPDLNAAIVADRLQLFASPDRQLMLSGQAKLANVNEQLRIDGKFMVDKALFDLP 1052 ++LG +PDLNA + A++L+LFASPDR+LMLSGQ K++NVNEQL +DGKF VD ALFDLP Sbjct: 966 IQLGADNPDLNATLTAEQLELFASPDRRLMLSGQGKISNVNEQLHVDGKFTVDHALFDLP 1025 Query: 1053 KSSAPKLGDDVVIVRKDKDGKSRAKAGAAATSKEKLTAATEKPAGRFAPVMNIVVDLGNN 1112 KSSAPKLGDDVVI+ + GK+ + G A+SK++L AATEKPA +FAP +NI VDLGN+ Sbjct: 1026 KSSAPKLGDDVVII--SRSGKN--QVGGQASSKQRLEAATEKPASKFAPFINIAVDLGND 1081 Query: 1113 FRFNGSGADLKLRGEMNVRSEPLLPLRATGTVRVSEGTYEAFGTKLNIERGIINFQGPIS 1172 FRF GSGADL+LRGEM VRSEPL P+RA+GT+ V+EG+YEAFGTKLNIERGIINFQGPI+ Sbjct: 1082 FRFRGSGADLRLRGEMAVRSEPLSPMRASGTINVAEGSYEAFGTKLNIERGIINFQGPIA 1141 Query: 1173 NPNLNILAMRRNQDVEAGVEVTGNANQPRVRLVSEPNVPDDEKLSWMMFGHGSDSSGLGQ 1232 NPN+NILAMRRNQDVEAGV VTGNANQPRV+LVSEPNVPDDEKLSWMMFGHG+D+S +GQ Sbjct: 1142 NPNINILAMRRNQDVEAGVSVTGNANQPRVQLVSEPNVPDDEKLSWMMFGHGTDNSSIGQ 1201 Query: 1233 RSASSQALAFVGNYGGKKIAKDIGLDQFSIGASESGLSDEQVVNLGKSISEKLSLGYEQS 1292 R+ASSQALA VGN GGK+IAKDIGLDQFSIG+SESGL+++QVVNLGK+I+EK++LGYEQS Sbjct: 1202 RTASSQALALVGNMGGKRIAKDIGLDQFSIGSSESGLTNDQVVNLGKAITEKITLGYEQS 1261 Query: 1293 LTGAASIAKATWQLSRRWSLVARTGTIFSIWIFYSQ 1328 L GAAS+AKATWQ+SRRWS+VAR GTI + I +++ Sbjct: 1262 LQGAASVAKATWQISRRWSVVARAGTINGLNILFNR 1297 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,105,757 Number of extensions: 175538 Number of successful extensions: 611 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 599 Number of HSP's successfully gapped: 1 Length of query: 1348 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1251 Effective length of database: 1,160,575 Effective search space: 1451879325 Effective search space used: 1451879325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 137 (57.4 bits)