BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0124.p (176 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 263 8e-72 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 122 2e-29 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 92 3e-20 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 86 2e-18 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 71 6e-14 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 263 bits (671), Expect = 8e-72, Method: Compositional matrix adjust. Identities = 135/188 (71%), Positives = 151/188 (80%), Gaps = 12/188 (6%) Query: 1 MAQTDAIGALLDVRPQYWKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQ 60 M DAI LDVR QYW+AIA F KP+LAAVNGYALGAGCE+LMHADIAIA RGAKIGQ Sbjct: 75 MIHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQ 134 Query: 61 PEINLGTIPGAGGTQRLIRTVGKPLAMKMVLSGEFLSADDALSAGLIAAAAGQG------ 114 PEIN+GT+PGAGGTQRLIRTVGKPLAMKMVLSGEF+SAD AL AGL+A Sbjct: 135 PEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERT 194 Query: 115 ---SHA---QVPLALRLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEK 168 +H+ + PLA+RLAKEAML+S+ELGLEAGL ERKSFSL+AAS DR+EGIAAF+EK Sbjct: 195 LALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEK 254 Query: 169 RTAQFVGR 176 R A F GR Sbjct: 255 RAAVFSGR 262 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 122 bits (305), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 70/171 (40%), Positives = 95/171 (55%), Gaps = 18/171 (10%) Query: 18 WKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQPEINLGTIPGAGGTQRL 77 W+ + KP++AAV GYALG GCE+ M D IA AK GQPEI LG +PGAGGTQRL Sbjct: 88 WETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRL 147 Query: 78 IRTVGKPLAMKMVLSGEFLSADDALSAGLIA---------------AAAGQGSHAQVPLA 122 R V K AM + L+G + A++A AGL++ A QV + Sbjct: 148 PRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAM- 206 Query: 123 LRLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEKRTAQF 173 + K+++ ++YE L G+ ER F A+ED++EG+ AF EKR F Sbjct: 207 --MVKDSVNRAYETTLSEGMKYERALFYSCFATEDQKEGMKAFLEKRQPVF 255 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 91.7 bits (226), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 59/168 (35%), Positives = 92/168 (54%), Gaps = 12/168 (7%) Query: 18 WKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQPEINLGTIPGAGGTQRL 77 ++ ++ F +AA+NGYA+G G E + DI IA A++ PE +G +P AGGTQ L Sbjct: 90 FETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMALPEAAVGLLPCAGGTQNL 149 Query: 78 IRTVGKPLAMKMVLSGEFLSADDALSAGLI------------AAAAGQGSHAQVPLALRL 125 VG+ A +M+L GE ++A+ AL GL+ A A Q + Q P ++ Sbjct: 150 PWLVGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAA 209 Query: 126 AKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEKRTAQF 173 +K+ + + L L ER+ F L ++D+REG+ AF EKR+ Q+ Sbjct: 210 SKQLIQGARHNPLGTILQTERELFVDLFDTQDQREGVQAFLEKRSPQW 257 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 85.9 bits (211), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 64/170 (37%), Positives = 80/170 (47%), Gaps = 12/170 (7%) Query: 19 KAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQPEINLGTIPGAGGTQRLI 78 K++ P++ AVNG A GAG + + DI +A R A + LG IP GGT L Sbjct: 94 KSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLP 153 Query: 79 RTVGKPLAMKMVLSGEFLSADDALSAGLI-----------AAAAGQGSHAQVPL-ALRLA 126 R VG AM M L GE +SA+ A GLI A A A+ P L Sbjct: 154 RLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAAT 213 Query: 127 KEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEKRTAQFVGR 176 K + S L L+LER + L S D REG+ AF EKR QF G+ Sbjct: 214 KATLYASPAHTLPEQLDLERDTMRALGRSRDYREGVTAFLEKRAPQFTGQ 263 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 70.9 bits (172), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 50/170 (29%), Positives = 75/170 (44%), Gaps = 12/170 (7%) Query: 16 QYWKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQPEINLGTIPGAGGTQ 75 Q+ I+ SKP++AAV+G A+G G +L+H D+ A AK P LG P A + Sbjct: 83 QFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADNAKFSMPFTQLGLCPEAASSL 142 Query: 76 RLIRTVGKPLAMKMVLSGEFLSADDALSAGLIAAAAGQ------------GSHAQVPLAL 123 L G A + +L GE AD+A G + Q A ++ Sbjct: 143 LLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMAQEELLPYAQQQAAKLAALPAASI 202 Query: 124 RLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEKRTAQF 173 R+ K+ M K+ E + E + FS + + + +E AF EKR F Sbjct: 203 RVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFTAFFEKRKPDF 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 500,214 Number of extensions: 19146 Number of successful extensions: 83 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 81 Number of HSP's successfully gapped: 7 Length of query: 176 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 97 Effective length of database: 1,245,805 Effective search space: 120843085 Effective search space used: 120843085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)