BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0123.p (158 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herba... 267 3e-73 gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [H... 254 2e-69 gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Her... 93 1e-20 gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [H... 90 9e-20 gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [H... 86 2e-18 >gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 267 bits (683), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 126/152 (82%), Positives = 141/152 (92%) Query: 1 MEAFICDAIRTPFGRFGGALSGVRADDLAALPIRALIERNPGVDWTKVDDIIYGCANQAG 60 MEA ICDAIRTPFGR+GGAL VRADDLAA PIR+L+ERNPGVDW++V+DI+YGCANQAG Sbjct: 1 MEALICDAIRTPFGRYGGALGAVRADDLAAAPIRSLMERNPGVDWSRVEDILYGCANQAG 60 Query: 61 EDNRNVARMAGLLAGLPVEVPGTTLNRLCGSSMDAVGTAARAIKAGECDLVIAGGVESMT 120 EDNRNVARMAGLLAGLP+ VPG+T+NRLCGSS+DAVG AARAIK+GE L+IAGGVESMT Sbjct: 61 EDNRNVARMAGLLAGLPIAVPGSTVNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMT 120 Query: 121 RAPFVMAKAESAFSRSAKIEDTTIGWRFINPL 152 RAPFVM KAESAF+RSA I DTTIGWRF+NPL Sbjct: 121 RAPFVMGKAESAFARSAAIFDTTIGWRFVNPL 152 >gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 254 bits (649), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 119/151 (78%), Positives = 134/151 (88%) Query: 2 EAFICDAIRTPFGRFGGALSGVRADDLAALPIRALIERNPGVDWTKVDDIIYGCANQAGE 61 + FICDAIRTP GR+GGAL VRADDL A+P+RAL+ERNP VDW VDD+IYGCANQAGE Sbjct: 3 DVFICDAIRTPIGRYGGALKDVRADDLGAVPLRALMERNPQVDWKAVDDVIYGCANQAGE 62 Query: 62 DNRNVARMAGLLAGLPVEVPGTTLNRLCGSSMDAVGTAARAIKAGECDLVIAGGVESMTR 121 DNRNVARM+ LLAGLP EVPG+T+NRLCGS MDA+GTAARAIK+GE L+IAGGVESMTR Sbjct: 63 DNRNVARMSLLLAGLPQEVPGSTINRLCGSGMDALGTAARAIKSGETQLMIAGGVESMTR 122 Query: 122 APFVMAKAESAFSRSAKIEDTTIGWRFINPL 152 APFVM KA+SAFSR A I+DTTIGWRF+N L Sbjct: 123 APFVMGKADSAFSRQAAIQDTTIGWRFVNAL 153 >gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 92.8 bits (229), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 51/125 (40%), Positives = 72/125 (57%), Gaps = 1/125 (0%) Query: 2 EAFICDAIRTPFGRFG-GALSGVRADDLAALPIRALIERNPGVDWTKVDDIIYGCANQAG 60 +A+I A RTP G+ G R DDL I++ + + PG+D ++D I GC+ G Sbjct: 7 DAYIVAATRTPIGKAPRGMFKNTRPDDLLVRAIQSAVAQVPGLDPKLIEDAIVGCSFPEG 66 Query: 61 EDNRNVARMAGLLAGLPVEVPGTTLNRLCGSSMDAVGTAARAIKAGECDLVIAGGVESMT 120 N+AR A LLAGLP + G T+NR C S + A+ AA I+ GE D++IA G ESM+ Sbjct: 67 AQGLNMARNAVLLAGLPNTIGGVTINRYCASGITAIAMAADRIRVGEADVMIAAGAESMS 126 Query: 121 RAPFV 125 P + Sbjct: 127 MVPMM 131 >gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 391 Score = 89.7 bits (221), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 56/144 (38%), Positives = 84/144 (58%), Gaps = 3/144 (2%) Query: 2 EAFICDAIRTPFGRFGGALSGVRADDLAALPIRALIERNPGVDWTKVDDIIYGCANQAGE 61 + I A RT G+FGGALS + A DL A I+AL+ + G+ + ++I G AG Sbjct: 3 DVVIVAAQRTAIGKFGGALSKIAAADLGAQVIKALLAKT-GIKPEAISEVILGQVLTAGL 61 Query: 62 DNRNVARMAGLLAGLPVEVPGTTLNRLCGSSMDAVGTAARAIKAGECDLVIAGGVESMTR 121 +N AR + + +GLP VP + ++CGS + AV AA+AI+ G+ +VIAGG E+M+ Sbjct: 62 -GQNPARQSVIKSGLPDMVPAFVVGKVCGSGLKAVQLAAQAIRCGDAQIVIAGGQENMSA 120 Query: 122 APFVMAKAESAFSR-SAKIEDTTI 144 +P V+ + F AK+ DT I Sbjct: 121 SPHVLNNSRDGFRMGDAKLTDTMI 144 >gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 85.5 bits (210), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 52/131 (39%), Positives = 74/131 (56%), Gaps = 3/131 (2%) Query: 5 ICDAIRTPFGRFGGALSGVRADDLAALPIRALIERN--PGVDWTKVDDIIYGCANQAGED 62 I A RT G F GAL+ + A L A+ I A ++R +++ + GC QAG Sbjct: 8 IVSAARTAMGSFQGALADLPAPHLGAVAISAALQRAQLSAAQIAQIEQVWMGCVLQAGL- 66 Query: 63 NRNVARMAGLLAGLPVEVPGTTLNRLCGSSMDAVGTAARAIKAGECDLVIAGGVESMTRA 122 + AR A L AGLP V TLN++CGS+M AV +I AG +V+AGG+ESM+ A Sbjct: 67 GQAPARQAALRAGLPQTVACATLNKVCGSAMQAVMLGHDSILAGSASMVVAGGMESMSNA 126 Query: 123 PFVMAKAESAF 133 P+++ KA + Sbjct: 127 PYLLPKARRGY 137 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 491,121 Number of extensions: 19823 Number of successful extensions: 85 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 83 Number of HSP's successfully gapped: 6 Length of query: 158 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 80 Effective length of database: 1,250,540 Effective search space: 100043200 Effective search space used: 100043200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)