BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0012.0122.p
         (447 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbas...   762   0.0  
gi|300311876|ref|YP_003775968.1| phenylacetyl-CoA-ligase [Herbas...   112   4e-26

>gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 446

 Score =  762 bits (1968), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 363/437 (83%), Positives = 402/437 (91%), Gaps = 2/437 (0%)

Query: 1   MPQRTPQPGDLEAIETASKDELQALQLQRMKWSLKHAYDNVPHYRASFDAAGVHPDDLKT 60
           M  R PQPG+LE IETAS+DELQALQLQR+KWSL+HAYDNVPHYR +FD AGVHPDDLK+
Sbjct: 1   MTIRIPQPGELEPIETASRDELQALQLQRLKWSLRHAYDNVPHYRNAFDQAGVHPDDLKS 60

Query: 61  LADLAKFPFTGKKDLRDNYPFNMFAVPREQVVRIHASSGTTGKPTVVGYTQNDIDTWATV 120
           L+DL++FPFT KKDLRD+YPF MFAVPREQVVR+HASSGTTGKPTVVGYTQNDIDTWATV
Sbjct: 61  LSDLSRFPFTTKKDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120

Query: 121 VARSIRAAGGRKGDIVHISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIQDF 180
           VARSIRAAGGR+GDIV ISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLI+DF
Sbjct: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180

Query: 181 KPSIIMVTPSYMLNIIEEFQRQGLDAVDSSLKVGIFGAEPWTDAMRAEIQARAGIDAVDI 240
           KPSIIMVTPSYMLN+IEEFQRQG+D   SSLKVGIFGAEPWTDAMR EIQ RAG+DAVDI
Sbjct: 181 KPSIIMVTPSYMLNVIEEFQRQGMDPASSSLKVGIFGAEPWTDAMRREIQQRAGLDAVDI 240

Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIINPETGEVLPDGSDGELVFTSLTKEAL 300
           YGLSEVMGPGVASECIESKDGPVIWEDHFYPEII+P+TGEVLPDG +GELVFTSL+KEAL
Sbjct: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGVEGELVFTSLSKEAL 300

Query: 301 PIIRYRTRDLTRLLPATSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKMPKLAPQ 360
           P+IRYRTRDLTRLLP TSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILK+P+LAPQ
Sbjct: 301 PMIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360

Query: 361 YQLVVGRNGHLDTLGVTVELRPEVALSAGDIDALARELQHHIKTHVGVTTAVQVVASGKI 420
           YQL + R+GH+D++ V  ELR +V  S   ++AL +ELQH IKT+VG++T V VV +G I
Sbjct: 361 YQLQIERHGHMDSMSVFAELREDV--SNEHVEALEKELQHSIKTYVGISTKVTVVPAGMI 418

Query: 421 ERSAVGKAKRVVDQRPK 437
           ER++VGKAKRV+D R K
Sbjct: 419 ERTSVGKAKRVIDNRKK 435


>gi|300311876|ref|YP_003775968.1| phenylacetyl-CoA-ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 423

 Score =  112 bits (281), Expect = 4e-26,   Method: Compositional matrix adjust.
 Identities = 106/363 (29%), Positives = 169/363 (46%), Gaps = 26/363 (7%)

Query: 52  GVHPDDLKTLADLAKFPFTGKKDLRD----NYPFN-MFAVPREQVVRIHASSGTTGKPTV 106
           GV    + + A LA+ P T K DL++      PF  + A P  Q+ R+  S G    P  
Sbjct: 49  GVDAAAISSRAALAQLPVTRKSDLKELQTRESPFGGLNATPTRQLRRLFVSPGPIFDPEG 108

Query: 107 VGYTQNDIDTWATVVARSIRAAGGRKGDIVHISYGYGLFTGGLGAHYGAEKLGCTVIPMS 166
            G      D W    A  ++A G + G I+   + Y            A +LGC VIP  
Sbjct: 109 HGA-----DWWR--FASPMKALGLQAGHILQNCFAYHFTPAAFMVEGAAARLGCAVIPAG 161

Query: 167 GGQTEKQVQLIQDFKPSIIMVTPSYMLNIIEEFQRQGLDAVDSSLKVGIFGAEPWTDAMR 226
            GQTE QV  +   KP   + TPS++  IIE+ Q  G D   SS++  +  AE    ++R
Sbjct: 162 IGQTELQVNAMAALKPDAYVGTPSFLKIIIEKAQEMGADI--SSVQRALVSAEALPPSLR 219

Query: 227 AEIQARAGIDAVDIYGLSEVMGPGVASECI-ESKDGP-VIWEDHFYPEIINPETGEVLPD 284
                      + +Y  +++    +A E + E    P ++ ++    EI+ P TG+ +  
Sbjct: 220 NWFHEHGVPQVLQLYASADIG--NIAYETMSEGAVHPGMVLDEALILEIVRPGTGDPVAA 277

Query: 285 GSDGELVFTSLTKEALPIIRYRTRDLTRLLPATS---RSMRRIGKITGRSDDMLIIRGVN 341
           G  GE+V TS   +  P+IR+ T DL+ ++P  S   R+  RI    GR+D    +RG+ 
Sbjct: 278 GEVGEVVVTSFNPD-YPLIRFGTGDLSAVVPGVSPCGRTNTRIKGWMGRADQTTKVRGMF 336

Query: 342 VFPTQIEELILKMPKLAPQYQLVVGRNGHLDTLGVTVEL-RPEVALSAGDIDALARELQH 400
           V P+Q+ +++ +   +  + +LVV      D + +  E+  PE    A D+ ALA  L+ 
Sbjct: 337 VHPSQVADVLRRHADVL-RARLVVSGEMANDVMTLHCEVGDPEN--PAIDVGALAGSLRE 393

Query: 401 HIK 403
             K
Sbjct: 394 VTK 396


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,495,176
Number of extensions: 66308
Number of successful extensions: 212
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 207
Number of HSP's successfully gapped: 2
Length of query: 447
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 359
Effective length of database: 1,203,190
Effective search space: 431945210
Effective search space used: 431945210
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 132 (55.5 bits)