BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0117.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 235 2e-63 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 87 2e-18 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 78 5e-16 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 69 2e-13 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 61 1e-10 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 235 bits (600), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 124/201 (61%), Positives = 137/201 (68%), Gaps = 43/201 (21%) Query: 1 MQYESILFENTDGVAVITLNRPDKLNSFTVAMHLEVRDALDKIAADPRVRNGVAAGAGAN 60 M SIL EN DG+AVITLNRPDKLNSFTVAMHLE+RDA+ + ADP VR + GAG Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 -------------------------------------------IXAVNGVAAGAGANIPL 77 I AVNGVAAGAGAN+PL Sbjct: 61 FCAGQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 Query: 78 ACDIVLAARSASFVEVFCKLGLLPDTGGTYFLPRLVGTARAMGLAMLGNKLSAEQAEQWG 137 ACDIVLAARSASFVEVFCKLGL+PDTGGTYFLPRLVGTARAMG+A+LG K+SAEQAE+WG Sbjct: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 Query: 138 LIWKCIDDDKLQTEARAMAQH 158 LIWKC+DDD+L EA AMA H Sbjct: 181 LIWKCVDDDQLMEEAHAMAAH 201 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 59/186 (31%), Positives = 86/186 (46%), Gaps = 38/186 (20%) Query: 1 MQYESILFENTDGVAVITLNRPDKLNSFTVAMHLEVRDALDKIAADPRV--------RNG 52 M YE+I E + V +I L+RP LN+ + + E+ +AL A + Sbjct: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 Query: 53 VAAGAGAN------------------------------IXAVNGVAAGAGANIPLACDIV 82 AAGA + I AV G A G G + + CD + Sbjct: 61 FAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI 120 Query: 83 LAARSASFVEVFCKLGLLPDTGGTYFLPRLVGTARAMGLAMLGNKLSAEQAEQWGLIWKC 142 +AA +A F + KLG++P GGT LPR V A+AM LA+ G + AE+AE+ GL+ + Sbjct: 121 IAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRI 180 Query: 143 IDDDKL 148 + DKL Sbjct: 181 VAADKL 186 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 78.2 bits (191), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 55/184 (29%), Positives = 82/184 (44%), Gaps = 37/184 (20%) Query: 1 MQYESILFENTDGVAVITLNRPDKLNSFTVAMHLEVRDALDKIAADPRVRNGVAAG---- 56 MQ + I DGV I +R DK N+ T AM+ ++ DAL + +D VR + G Sbjct: 1 MQMD-IQLSKADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKA 59 Query: 57 --AGAN------------------------------IXAVNGVAAGAGANIPLACDIVLA 84 AG + + AV+GVA G G + L CD+V A Sbjct: 60 FSAGNDLEDFLKNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYA 119 Query: 85 ARSASFVEVFCKLGLLPDTGGTYFLPRLVGTARAMGLAMLGNKLSAEQAEQWGLIWKCID 144 A +A F F +LGL P+ + LP + G RA +LG A++A Q G + + + Sbjct: 120 ADNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMA 179 Query: 145 DDKL 148 ++L Sbjct: 180 QEEL 183 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 69.3 bits (168), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 63/202 (31%), Positives = 86/202 (42%), Gaps = 39/202 (19%) Query: 3 YESILF-ENTDGVAVITLNRPDKLNSFTVAMHLEVRDALDKIAADPRVR--------NGV 53 YE +L + + V +TLNRP N+ E+ AL + D VR Sbjct: 7 YEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAF 66 Query: 54 AAGAGAN-----------------------------IXAVNGVAAGAGANIPLACDIVLA 84 AAGA N + AVNG A GAG + + DI +A Sbjct: 67 AAGADLNEMIHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIA 126 Query: 85 ARSASFVEVFCKLGLLPDTGGTYFLPRLVGTARAMGLAMLGNKLSAEQAEQWGLIWKCID 144 AR A + +G LP GGT L R VG AM + + G +SA+QA Q GL+ + +D Sbjct: 127 ARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVD 186 Query: 145 DDKLQTEARAMAQHXGARRRPC 166 DD A+A H A++ P Sbjct: 187 DDATLERTLALA-HSIAQKSPL 207 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 39/106 (36%), Positives = 57/106 (53%) Query: 53 VAAGAGANIXAVNGVAAGAGANIPLACDIVLAARSASFVEVFCKLGLLPDTGGTYFLPRL 112 ++A G +I A+NG A G G LACDI +A A +GLLP GGT LP L Sbjct: 93 LSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWL 152 Query: 113 VGTARAMGLAMLGNKLSAEQAEQWGLIWKCIDDDKLQTEARAMAQH 158 VG A + + G +++AE A + GL+ + + + + A A+AQ Sbjct: 153 VGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERALALAQQ 198 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.131 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 571,882 Number of extensions: 17325 Number of successful extensions: 77 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 79 Number of HSP's successfully gapped: 6 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)