BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0114.p (360 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 618 e-178 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 86 3e-18 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 67 2e-12 gi|300312623|ref|YP_003776715.1| chaperone activity ATPase [Herb... 60 3e-10 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 618 bits (1593), Expect = e-178, Method: Compositional matrix adjust. Identities = 294/360 (81%), Positives = 322/360 (89%), Gaps = 4/360 (1%) Query: 1 MSKFYPLTVGNVKNETRDTIVVSFDVPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSIC 60 MSKFYPLT+ +VK ETRDTIVVSF VPAEL+ TF YQQGQHLTLRSEINGEDLRRSYSIC Sbjct: 1 MSKFYPLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAA 120 SAVQ+ QLRVAIKR GG+FS WAN++ PGQ + VMPPMGHFN+PL+A N+KHYLAFAA Sbjct: 61 SAVQERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAA 120 Query: 121 GSGITPMLSIIKTTLLAEPGSRFTLVYGNRASSSVSSVIFKEELTDLKDIYLERLNVVYV 180 GSGITPM+SIIKTTLL+EP S FTLVY NRAS SSVIFKEELTDLKD YLER NVVYV Sbjct: 121 GSGITPMMSIIKTTLLSEPHSHFTLVYANRAS---SSVIFKEELTDLKDAYLERFNVVYV 177 Query: 181 MSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAAFICGPEEMLNAVSASLQANGLDKS 240 MSREQQDVELFNGRI R KCD FF SWIDL D+DAAF+CGPEEM+ AV+ASLQA+GL K+ Sbjct: 178 MSREQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEEMVQAVAASLQAHGLPKT 237 Query: 241 AIKVELFAASIPKHKAVAARPVVGKQECEVTVIVDGYHTVFTMEKEKESLLDAGLKNGID 300 IK ELFAAS P +A AAR VVGKQECEVTVIVDGYH VFTM+KEKES+L+AGLK+GID Sbjct: 238 QIKTELFAASTPP-RAHAARTVVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGID 296 Query: 301 MRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQSFPVTDKLLIDFDQDN 360 +RYSCKGGVCATCRCK+VEGKVDMDANY LEDYEIARGFVLSCQSFPV+DKLL+DFDQDN Sbjct: 297 LRYSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN 356 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 86.3 bits (212), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 66/239 (27%), Positives = 110/239 (46%), Gaps = 20/239 (8%) Query: 21 VVSFD-VPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSICS----AVQDDQLRVAIKRT 75 + SF VP + + + GQ++ LR ++GE+ RR YS+ + A Q Q R+++KR Sbjct: 170 ITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQPPQYRISVKRE 229 Query: 76 QGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAAGSGITPMLSIIKTTL 135 GG S +D + G +++ PP G F L A+ K + G GITPM+S++ L Sbjct: 230 PGGKVSRQLHDQIHVGSVVELFPPAGEFTL---MASDKPLALISGGVGITPMMSMLAAAL 286 Query: 136 LAEPGSRFTLVYGNRASSSVSSVIFKEELTDLKDIYLE-RLNVVYVMSREQQDVELFNGR 194 + G ++ R + F+ +L +L + + +L Y R+ G Sbjct: 287 PS--GRPIHFIHAARHGGVHA---FRRQLEELAARHPQLQLRFCYEQPRQGDAAPHATGL 341 Query: 195 ITREKCDQFFKSWIDLDDIDAAFICGPEEMLNAVSASLQANGLDKSAIKVELF--AASI 251 I R Q+ DLD A+ GP+ + A+ L G+ + + E F AAS+ Sbjct: 342 IERSLLQQWLPGNPDLD----AYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFGPAASL 396 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 72/315 (22%), Positives = 130/315 (41%), Gaps = 55/315 (17%) Query: 53 LRRSYSICSAVQD-DQLRVAIKR---TQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLD 108 L R YS+C+A + + ++ + R ++GG S ++ LK G L + P F L Sbjct: 48 LVRQYSLCNAPHEQHRYQIGVLRDVNSRGG--SVAVHEALKLGDILTISAPRNQFAL--- 102 Query: 109 AANQKHYLAFAAGSGITPMLSIIKTTLLAEPGSRFTLVYGNRASSSVSSVIFKEELTDLK 168 +H L A G G+TP+L + + A + F + Y R+ + + F+E + Sbjct: 103 -VPAQHSLLLAGGIGVTPILCMAEALAAAG--ASFEMHYCARSPARQA---FRERIA--- 153 Query: 169 DIYLERLNVVYVMSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAA-------FICGP 221 F R+ D +DLD + A ++CGP Sbjct: 154 -------------------ASGFAARVVHHYDDGEAAQKLDLDALLAQADPATHLYVCGP 194 Query: 222 EEMLNAVSASLQANGLDKSAIKVELFAASIPKHKAVAARPVVGKQECEVTVIVDGYHTVF 281 + V + +A G ++ + E F A+ K G EV + G V+ Sbjct: 195 AGFIAHVVDTARARGWPEAQVHFEYFGAAPVKAD--------GNGAFEVKLASSG--QVY 244 Query: 282 TMEKEKESLLDAGLKNGIDMRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVL 341 T+ E+ +++ ++G+D+ SC+ G+C TC +++EG+ D Y ++ A Sbjct: 245 TIPPER-TVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFT 303 Query: 342 SCQSFPVTDKLLIDF 356 C S + L++D Sbjct: 304 PCCSRARSALLVLDL 318 >gi|300312623|ref|YP_003776715.1| chaperone activity ATPase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 59.7 bits (143), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 43/146 (29%), Positives = 74/146 (50%), Gaps = 8/146 (5%) Query: 6 PLTVGNVKNETRDTIVVSFDVPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSICSAV-Q 64 P+ V + D IV+S +PA + QY+ GQ++ + + RRSYS+ +A + Sbjct: 104 PVRVAKLDKVADDVIVLSLQLPANDR--LQYKAGQYVEF---LLRDGKRRSYSMATAPHK 158 Query: 65 DDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAAGSGI 124 D+ + + I+ GG+F+ TLK L++ P+G F L D + K + A+G+G Sbjct: 159 DEHMSLHIRHLPGGLFTDQVFTTLKERDILRIEGPLGTFFLRED--SDKPIILLASGTGF 216 Query: 125 TPMLSIIKTTLLAEPGSRFTLVYGNR 150 P+ +I++ A TL +G R Sbjct: 217 APIKAIVEQLEHAGSKRPVTLYWGGR 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,031,287 Number of extensions: 39296 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 5 Length of query: 360 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 274 Effective length of database: 1,212,660 Effective search space: 332268840 Effective search space used: 332268840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)