BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0089.p (326 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 649 0.0 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 209 3e-55 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 124 9e-30 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 112 4e-26 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 107 1e-24 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 309/326 (94%), Positives = 318/326 (97%) Query: 1 VFEGVPTYPNAGRFWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLG 60 VFEGVPTYPNAGRFWDMV RHKATIFYTAPTAIRSLIKAA+ADEK+HPKQYDLSSLR+LG Sbjct: 335 VFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLSSLRILG 394 Query: 61 SVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI 120 SVGEPINPEAWMWYYKN+G E CPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI Sbjct: 395 SVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI 454 Query: 121 TAAIVDETGNDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPEEFGGKVYLAGDGA 180 TAAIVDETGND+PNG GGILVVKRPWPSMIRTIWNDPERFKKSYFPEE GGK+YLAGDGA Sbjct: 455 TAAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGA 514 Query: 181 IRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGESICA 240 +RNK+TGYFTITGRIDDVLNVSGHRMGTMEIESALVAN IVAEAAVVGKPDETTGESICA Sbjct: 515 VRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICA 574 Query: 241 FVVLKRPRPTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVL 300 FVVLKRPRPTGDEAK IAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVL Sbjct: 575 FVVLKRPRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVL 634 Query: 301 AKGEDITQDISTLENPAILDQLKEAQ 326 AKGE ITQD+STLENPAILDQLKEAQ Sbjct: 635 AKGEAITQDVSTLENPAILDQLKEAQ 660 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 209 bits (531), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 129/330 (39%), Positives = 197/330 (59%), Gaps = 18/330 (5%) Query: 1 VFEGVPTYPNAGRFWDMVQRHKATIFYTAPTAIRSLIKAA-EADEKIHPKQYDLSSLRLL 59 ++EG+P P+AG +W +V+++K T ++APTAIR L K E E+ YD SSL+ L Sbjct: 305 LYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMER-----YDTSSLKAL 359 Query: 60 GSVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGA--TPQVPGSCTLPL 117 GEP++ W G K P++D +WQTE+G +IS G P GS +P+ Sbjct: 360 YLAGEPLDETTSSWIS---GALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSPGVPM 416 Query: 118 PGITAAIVDE-TGNDLPNGTGGILVVKRPWP-SMIRTIWNDPERFKKSYFPEEFGGKVYL 175 PG AI+DE TG G++ ++ P P ++T++ D ERF +Y+ +VY Sbjct: 417 PGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYW-RSLPREVYS 475 Query: 176 AGDGAIRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTG 235 D IR+KD GY+ I GR DDV+NV+GHR+GT EIE ++ ++P V+E AVVG D+ G Sbjct: 476 TFDWGIRDKD-GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKG 534 Query: 236 ESICAFVVLKRPR--PTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIM 293 + AF + K+ T ++ KA+ E+ V K+IG + +P + F LPKTRSGK++ Sbjct: 535 QVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRSGKLL 594 Query: 294 RRLLRVLAKGEDITQDISTLENPAILDQLK 323 RR ++ + +G D D+ T+E+PA L+Q++ Sbjct: 595 RRAIQSICEGRD-PGDLPTIEDPAALEQVR 623 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 124 bits (311), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 99/290 (34%), Positives = 142/290 (48%), Gaps = 21/290 (7%) Query: 10 NAGRFWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLGSVGEPINPE 69 +A + ++ RH+ + PTA++ L AA Q D S LR + S GE + + Sbjct: 276 DAAEVFGLLARHQIRNVFFPPTALKMLRGAATVR-----AQADFS-LRSVASGGETLGDD 329 Query: 70 AWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETG 129 W + +G I + + QTE ++S P V GS +PG IVDE G Sbjct: 330 LIAWGREALG---VTINEFYGQTECN-LVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQG 385 Query: 130 NDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPEEFGGKVYLAGD-GAIRNKDTGY 188 LP+GT G + ++ P P M W + E + E+F G L GD G++ + GY Sbjct: 386 QVLPHGTVGNIAIRAPDPVMFLRYWRNEEATR-----EKFAGDFLLTGDLGSM--DEQGY 438 Query: 189 FTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGESICAFVVLKRPR 248 GR DDV+ +G+R+G IE L+ +P V AAVVG D E + AFVVLK Sbjct: 439 IRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDG- 497 Query: 249 PTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLR 298 T DE A+ EL+ V ++ P+ I F LP T +GKIMR+ L+ Sbjct: 498 VTPDE--ALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLK 545 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 112 bits (280), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 79/246 (32%), Positives = 117/246 (47%), Gaps = 19/246 (7%) Query: 56 LRLLGSVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTL 115 LR + S GEP+N E W+ + PI D + QTE G + + VPGS Sbjct: 316 LRAVSSAGEPLNAEVVRWFASAL---DVPIHDHYGQTELGMVVNNHHALRHEVVPGSAGF 372 Query: 116 PLPGITAAIVDETGNDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPEE---FGGK 172 +PG ++DE +L G L + I P + Y+ ++ Sbjct: 373 AMPGYRVVVLDENHQELGANQPGELAID---------IARSPLYWFSGYWKQDTPAIAHG 423 Query: 173 VYLAGDGAIRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDE 232 Y GD + + G + GR DDV+ +G+R+G ++ESAL+ +P VA+ AV+G PD Sbjct: 424 YYSTGDN-VELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLPDP 482 Query: 233 TTGESICAFVVLKRPRPTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKI 292 E + AFVVL D A+ ++L V + + A P+ I F D LPKT SGK+ Sbjct: 483 ERTEIVKAFVVLSDQFKASD---ALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKL 539 Query: 293 MRRLLR 298 R +LR Sbjct: 540 QRFVLR 545 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 87/289 (30%), Positives = 131/289 (45%), Gaps = 32/289 (11%) Query: 14 FWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLGSVGEPINPEAWMW 73 F + ++K F T +L+ A+ + D S R G + Sbjct: 294 FVKELAKYKVNTFPAVNTLYNALLNNADF------ARLDFSGYRCCVGGGMAVQKSVADK 347 Query: 74 YYKNIGQEKCPIVDTFWQTETGGHMISPLPGATP----QVPGSCTLPLPGITAAIVDETG 129 + K G CPI++ + +ET SP+ A P + G+ LPLP AI+D+ G Sbjct: 348 WKKLTG---CPIIEGYGLSET-----SPIASANPCTIDEYTGTIGLPLPSTEFAILDDDG 399 Query: 130 NDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPEEFGGKVYLAGDGAIRNKDTGYF 189 +P G G + ++ P ++ W P+ K P+ F + GD + + + GY Sbjct: 400 QPVPLGQPGEIAIR--GPQVMPGYWQRPDETAKVMTPDGF----FKTGDIGVMD-ERGYT 452 Query: 190 TITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGESICAFVVLKRPRP 249 I R D++ VSG + E+E + +P V E A VG PDE TGE++ FVV K Sbjct: 453 KIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEAVKLFVVRK---- 508 Query: 250 TGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLR 298 + A +L D+ ++ KPK I F D LPKT GKI+RR LR Sbjct: 509 ---DKTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,164,286 Number of extensions: 53512 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 9 Length of query: 326 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 241 Effective length of database: 1,217,395 Effective search space: 293392195 Effective search space used: 293392195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)