BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0079.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 330 6e-92 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 105 5e-24 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 100 9e-23 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 88 8e-19 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 70 1e-13 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 330 bits (846), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 160/245 (65%), Positives = 188/245 (76%), Gaps = 1/245 (0%) Query: 1 MTYHASNTRYE-TMQYRQCGRSGLKLPLLSLGMWHNFGDTTPLATQREMLRTAFDLGITH 59 M+Y A +RY+ M YR+ GRSGL LP +SLG+W NFG T R +LR AFD G+TH Sbjct: 1 MSYIAEPSRYDGRMPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTH 60 Query: 60 FDLANNYGPPYGSAEANFGHLFKQDFQPYRDELIISTKAGWDMWPGPYGQGGGSRKYVLA 119 FDLANNYGPP GSAE NFG +DF PYRDEL+IS+KAGW MWPGPYG G+RK+++A Sbjct: 61 FDLANNYGPPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIA 120 Query: 120 SLDQSLKRMNLDYVDIFYSHRFDPETPLEETMGALATAVQQGKALYVGVSSYSPEKTREA 179 S +QSLKRM LDYVDIFYSHR D +TPLEETMGALA +QGKALYVG+SSYSPE TR+A Sbjct: 121 SCEQSLKRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKA 180 Query: 180 AELLKQWKVPCLIHQPSYNMFNRWIEQGLLDELERQGMGCITFTALAQGLLSDKYLNGIP 239 A +LK VP LIHQP+Y+M NR IE GLL LE G+GCI F+ LAQGLL+ KYL G+P Sbjct: 181 AAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVP 240 Query: 240 DDAAV 244 + A V Sbjct: 241 EGARV 245 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 105 bits (261), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 72/226 (31%), Positives = 114/226 (50%), Gaps = 9/226 (3%) Query: 13 MQYRQCGRSGLKLPLLSLGMWHNFGDTTPLATQREMLRTAFDLGITHFDLANNYGPPYGS 72 M YR+ G+S LK+ L LG FGD T L ++ +A + G+ D A+ Y G Sbjct: 1 MHYRRLGKSNLKVSALCLGSMM-FGDQTDLQESGRIVASAREHGVNFIDTADVYTK--GK 57 Query: 73 AEANFGHLFKQDFQPYRDELIISTKAGWDMWPGPYGQGGGSRKYVLASLDQSLKRMNLDY 132 +E G L R E ++++K G M P + SR +++ + L R++ DY Sbjct: 58 SEEIVGKLLAGQ----RHEWVLASKLGNAMSGKP-NESNYSRSWIVRETENILTRLDTDY 112 Query: 133 VDIFYSHRFDPETPLEETMGALATAVQQGKALYVGVSSYSPEKTREAAELLKQWKVPC-L 191 +DI Y HR + LEE + AL ++ GK GVS++ + E L ++ VP L Sbjct: 113 LDILYMHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPL 172 Query: 192 IHQPSYNMFNRWIEQGLLDELERQGMGCITFTALAQGLLSDKYLNG 237 + QP YN+ NR E +L G+G + ++ +A+G+L+ KY G Sbjct: 173 VCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPG 218 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 100 bits (250), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 74/225 (32%), Positives = 112/225 (49%), Gaps = 25/225 (11%) Query: 16 RQCGRSGLKLPLLSLG------MWHNFGDTTPLATQREMLRTAFDLGITHFDLANNYGPP 69 R+ G +G + + LG + D LAT + A +LGI D A+ YGP Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLAT----IDRALELGINLLDTADMYGP- 72 Query: 70 YGSAEANFGHLFKQDFQPYRDELIISTKAGWDMWPGPYGQGG--GSRKYVLASLDQSLKR 127 Y + E L + + RD+ I+TK G P G G GS Y+ +++ SLKR Sbjct: 73 YTNEE-----LLGRAIKGRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKR 127 Query: 128 MNLDYVDIFYSHRFDPETPLEETMGALATAVQQGKALYVGVSSYSPEKTREAAELLKQWK 187 + ++ +D++Y HR DP TP+E TMG LA VQ GK Y+G+S S AA L + + Sbjct: 128 LGVETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEAS------AATLERAHR 181 Query: 188 V-PCLIHQPSYNMFNRWIEQGLLDELERQGMGCITFTALAQGLLS 231 V P Q Y+++ R E +L G+G + ++ L +G L+ Sbjct: 182 VHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLT 226 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 87.8 bits (216), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 79/254 (31%), Positives = 121/254 (47%), Gaps = 32/254 (12%) Query: 13 MQYRQCGRSGLKLPLLSLGMWHNFGDTTPLATQREMLRTAFDLGITHFDLANNYGPP--- 69 MQYR GR+ LK+ L++LG +G+ A L A + GI D+A Y P Sbjct: 1 MQYRPLGRTDLKVSLITLGTM-TWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKA 59 Query: 70 --YGSAEANFGHLFKQDFQPYRDELIISTKA-GWDMWP-GPYGQGGG----SRKYVLASL 121 G E G K+ + RD+L+I+TK G P P GG RK + +L Sbjct: 60 ETQGLTERYLGTWLKKSGK--RDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEAL 117 Query: 122 DQSLKRMNLDYVDIF----------------YSHRFDPET-PLEETMGALATAVQQGKAL 164 SL+R+ LD+VD++ Y H D T P+EET+ L+ V+ GK Sbjct: 118 HGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIR 177 Query: 165 YVGVSSYSPEKTREAAELLKQWKVPCLIH-QPSYNMFNRWIEQGLLDELERQGMGCITFT 223 +G+S+ + + +Q + ++ Q YN+ NR E L + R+ +G + ++ Sbjct: 178 TIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYS 237 Query: 224 ALAQGLLSDKYLNG 237 LA G+LS KYL+G Sbjct: 238 PLAFGMLSGKYLDG 251 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 70.5 bits (171), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 65/228 (28%), Positives = 106/228 (46%), Gaps = 22/228 (9%) Query: 13 MQYRQCGRSGLKLPLLSLGMWHNFGDTTPLATQ--REMLRTAFDLGITHFDLANNYGPPY 70 M+ R+ GR+G ++ + G W G ++ Q + L A D G++ D A+ YG Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGD-- 58 Query: 71 GSAEANFGHLFKQ--DFQPYRDELIISTKAGWDMWPGPYGQGGGSRKYVLASLDQSLKRM 128 G +E + K+ +P+ ++TKAG + P G R + A +D+SLK + Sbjct: 59 GRSEQLIAQVLKERGGERPF-----VATKAGRRLSPH-VASGYNDRAQLSAFIDRSLKNL 112 Query: 129 NLDYVDIFYSHRFDPETPL---EETMGALATAVQQGKALYVGVSSYSPEKTREAAELLKQ 185 +D +D+ H P T + +E G L V GK + GVS EK EA + ++ Sbjct: 113 GVDCLDLVQLH--CPPTEVYYQQEVFGHLDALVAAGKIRHYGVSV---EKVEEALKAIEY 167 Query: 186 WKVPCLIHQPSYNMFNRWIEQGLLDELERQGMGCITFTALAQGLLSDK 233 V + Q +N+F + L E +R+ + I LA GLL+ K Sbjct: 168 PNVKSV--QIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGK 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 853,867 Number of extensions: 37043 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 89 Number of HSP's successfully gapped: 6 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)