BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0073.p (161 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312800|ref|YP_003776892.1| transcription regulator protein... 133 9e-33 >gi|300312800|ref|YP_003776892.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 147 Score = 133 bits (334), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 71/144 (49%), Positives = 91/144 (63%), Gaps = 2/144 (1%) Query: 13 MKKDSRLSGVLHVLLHLAEH-PTPMTSESLAKAMDTNPVVIRRIMAGLREQGYVSSEKGH 71 MK+ RLS LH+LLH+ + PMTSE +A ++TNPVVIRR AGLRE G V+S KGH Sbjct: 1 MKRSDRLSIALHILLHMGQRTDAPMTSEEIAGWVNTNPVVIRRTFAGLREAGIVTSTKGH 60 Query: 72 GGGWTLTCDLAQITLRDVYVALGSPSLLAIGSRNEAPACLVEQAVNAALNKTFAEAEELL 131 GGGW+L + +I+L V ALG L+ +G+R E+P CLVE AVN AL+ EA +L Sbjct: 61 GGGWSLARPMEEISLLQVQQALGE-RLVTLGAREESPGCLVEIAVNRALDDVMEEANRIL 119 Query: 132 VSRFGEVTLAMLSADFHQRFEKKR 155 R +TLA L+ D F R Sbjct: 120 DQRLAGITLAALAQDVGPHFHTWR 143 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 471,227 Number of extensions: 18344 Number of successful extensions: 62 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 60 Number of HSP's successfully gapped: 1 Length of query: 161 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 83 Effective length of database: 1,250,540 Effective search space: 103794820 Effective search space used: 103794820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)