BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0068.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 207 6e-55 gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 197 5e-52 gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 175 3e-45 gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 171 6e-44 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 164 6e-42 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 207 bits (528), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 115/259 (44%), Positives = 162/259 (62%), Gaps = 5/259 (1%) Query: 10 ILFTAALAFSANVFAADA----PLRVGASPGPFSDFLQEAAKIANAQGFPVKVIEFTEPT 65 I F A L +A + +A A +++G + GP ++ +++ K+ G +KVIEFT+ Sbjct: 7 IQFIAGLGLAAGLVSAPAFAQDQIKMGVTAGPHAEIMEQVKKLLEKDGVQMKVIEFTDYI 66 Query: 66 QINEATQAGDIDLNNFQHVPYMQKQNESRGYKIVSIKPTYVAPAGIYSARHKSLAAFPQG 125 Q N A AGD+D N++QH PY+ Q + RGYK VS+ T P G+YS + KSL QG Sbjct: 67 QPNAALAAGDLDANSYQHQPYLDAQIKDRGYKFVSVGSTITFPMGVYSKKIKSLNDLKQG 126 Query: 126 AKIGIPNDPANEARALFLLQKAGLIKLKADANTNAGVADIAENPKKLNIVLLDEVQLPRV 185 A++G+PNDP N RAL +LQ G+IKLKADA A DI ENPKK+ IV LD QLPR Sbjct: 127 ARVGVPNDPTNGGRALLVLQAKGVIKLKADAGLKATPLDIVENPKKIKIVELDAAQLPRS 186 Query: 186 LVDIDAGVITLNKGVLAGLNP-KDALLLEDKTSQWAIIWAARADRKDDPRIARFIKIFES 244 L D DA VI N AGL+P KDA+ +E T +A + A R KD P +A+ +K + S Sbjct: 187 LDDFDAAVINGNYAESAGLSPTKDAIAVEASTGPYANVIAVRIADKDKPWVAKLVKAYHS 246 Query: 245 EQIKQFIIKKFNGTVIPAW 263 ++K+F+++K+ G+VI +W Sbjct: 247 PEVKKFVLEKYKGSVITSW 265 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 197 bits (502), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 104/264 (39%), Positives = 159/264 (60%), Gaps = 8/264 (3%) Query: 7 TKKILFTAALAFSANVFAADAPLRVGASPGPFSDFLQEAAKI--ANAQGFPVKVIEFTEP 64 T + A+L +A AAD +RVG + GP + ++A ++ + G+ VK+IEF + Sbjct: 14 TLMVSMLASLVLNAQA-AADQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKIIEFNDY 72 Query: 65 TQINEATQAGDIDLNNFQHVPYMQKQNESRGYKIVSIKPTYVAPAGIYSARHKSLAAFPQ 124 Q N A AG++D N++QH P++ Q ++RGYK+ + T + P IYS +++ L P Sbjct: 73 IQPNAALDAGELDANSYQHRPFLNAQIKTRGYKLYAEGKTMIGPMAIYSRKYRKLEDVPV 132 Query: 125 GAKIGIPNDPANEARALFLLQKAGLIKLKAD----ANTNAGVADIAENPKKLNIVLLDEV 180 GA+IGIPNDPANE+R L LLQK G IKL+A TNA DI ENPKK V +D Sbjct: 133 GARIGIPNDPANESRVLLLLQKHGFIKLRAGIDPLTGTNATPIDITENPKKWKFVEIDAA 192 Query: 181 QLPRVLVDIDAGVITLNKGVLAGLNP-KDALLLEDKTSQWAIIWAARADRKDDPRIARFI 239 QLPR L D+DA + + AGLNP +D+L++E S +A + A R + P + + + Sbjct: 193 QLPRTLDDLDASAVNADYAAKAGLNPARDSLVVESGDSPYACLIAVREKDRAQPWLGKLV 252 Query: 240 KIFESEQIKQFIIKKFNGTVIPAW 263 + ++S ++K++I +F G ++ AW Sbjct: 253 QAYQSPEVKRYIETEFKGGILAAW 276 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 175 bits (444), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 101/239 (42%), Positives = 146/239 (61%), Gaps = 4/239 (1%) Query: 29 LRVGASPGPFSDFLQEAAKIANAQGFPVKVIEFTEPTQINEATQAGDIDLNNFQHVPYMQ 88 ++VG S G K+A G ++ + F++ Q N A AGD+D N FQH PY++ Sbjct: 36 IKVGISVGSAEAIFDVVKKVAARDGLEIQTVVFSDYLQPNAALAAGDLDANAFQHRPYLE 95 Query: 89 KQNESRGYKIVSIKPTYVAPAGIYSARHKSLAAFPQGAKIGIPNDPANEARALFLLQKAG 148 Q +RGY+IV + T AP GIYS + +S+ PQGA IGI NDP+N RAL LLQKAG Sbjct: 96 SQIAARGYRIVPVGLTITAPLGIYSRKFRSVEQLPQGAAIGIQNDPSNGNRALLLLQKAG 155 Query: 149 LIKLK---ADANTNAGVADIAENPKKLNIVLLDEVQLPRVLVDIDAGVITLNKGVLAGLN 205 LI+LK + NA D+ ENP+KL +V LD QLPR L D+ A I + AGL+ Sbjct: 156 LIRLKPGVGENGVNATPLDVIENPRKLKLVELDAAQLPRSLDDLAAASINNDYAFKAGLS 215 Query: 206 -PKDALLLEDKTSQWAIIWAARADRKDDPRIARFIKIFESEQIKQFIIKKFNGTVIPAW 263 KD + +ED ++A I A RA+ KD P + + ++ + SE++++FI +F G+++PA+ Sbjct: 216 LQKDTIAVEDPRGRYANIIATRAEDKDRPWVRKLVQAYHSEEVRKFIETEFKGSLVPAF 274 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 171 bits (433), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 101/252 (40%), Positives = 139/252 (55%), Gaps = 3/252 (1%) Query: 14 AALAFSANVFAADAPLRVGASPGPFSDFLQEAAKIANAQGFPVKVIEFTEPTQINEATQA 73 AALAFS AAD L + A+P P ++ L+ +G ++V FT+ Q T Sbjct: 13 AALAFSVGAHAADK-LVIAATPVPHAEILEHIKPALAKEGIDLQVKVFTDYVQPAAQTNE 71 Query: 74 GDIDLNNFQHVPYMQKQNESRGYKI-VSIKPTYVAPAGIYSARHKSLAAFPQGAKIGIPN 132 +D N F H PY+ + +S I V + +V P YS ++K +A P GA I IPN Sbjct: 72 KQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIADIPNGATIAIPN 131 Query: 133 DPANEARALFLLQKAGLIKLKADANTNAGVADIAENPKKLNIVLLDEVQLPRVLVDIDAG 192 DP+N RAL LL + GLIKLK + N +A DI ENPKKL L+ LPRVL +D Sbjct: 132 DPSNSGRALLLLARNGLIKLKDNTNISATQKDIVENPKKLKFRALEAATLPRVLNQVDVA 191 Query: 193 VITLNKGVLAGLNP-KDALLLEDKTSQWAIIWAARADRKDDPRIARFIKIFESEQIKQFI 251 +I N + A LNP KD+L +ED S +A + AR D KD P I + S +K+FI Sbjct: 192 LINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAAALNSPDVKKFI 251 Query: 252 IKKFNGTVIPAW 263 +K+ G ++PA+ Sbjct: 252 EEKYQGAIVPAF 263 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 164 bits (416), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 97/251 (38%), Positives = 143/251 (56%), Gaps = 3/251 (1%) Query: 9 KILFTAALAFSANVFAADAPLRVGASPGPFSDFLQEAAKIANAQGFPVKVIEFTEPTQIN 68 ++F + S A LR+G PG + D + AAK A QG V+V+EFT+ T N Sbjct: 5 HLVFLSLTVLSLQAAQAADKLRIGVVPGAYGDSVAVAAKEAKRQGIDVQVVEFTDWTTPN 64 Query: 69 EATQAGDIDLNNFQHVPYMQKQNESRGYKIVSIKPTYVAPAGIYSARHKSLAAFPQGAKI 128 A AGD+DLN FQH P++ + GYK+ S ++ G+YS ++KSL A PQGAK+ Sbjct: 65 VALDAGDLDLNYFQHQPFLDNAIQKNGYKLASAGTGILSNVGLYSLKYKSLTALPQGAKV 124 Query: 129 GIPNDPANEARALFLLQKAGLIKLKADANTNAGVADIAENPKKLNIVLLDEVQLPRVLVD 188 GI NDP N+ RAL LLQ GLIKLK + DI +NP+KL+ V ++ QL R+ D Sbjct: 125 GIANDPVNQGRALLLLQNVGLIKLKPKVGYLGSIDDIIDNPRKLSFVEVEGPQLVRITPD 184 Query: 189 IDAGVITLNKGVLA-GLNPKDALLLED-KTSQWAIIWAARADRKDDPRIARFIKIFE-SE 245 +D + V A +P L + +++AI + RA+R +DP + +FI+I++ S Sbjct: 185 VDLAQGYPHFIVAAKAFDPSSGLAYSGIEDARFAIQFVTRANRVNDPVVQKFIQIYQNSA 244 Query: 246 QIKQFIIKKFN 256 ++ I + FN Sbjct: 245 AVRADIGRAFN 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 763,660 Number of extensions: 30746 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 5 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)