BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0065.p (210 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 91 7e-20 gi|300310541|ref|YP_003774633.1| nitrate transporter permease [H... 76 2e-15 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 70 1e-13 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 64 1e-11 gi|300311644|ref|YP_003775736.1| taurine ABC transporter permeas... 63 2e-11 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 90.9 bits (224), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 53/155 (34%), Positives = 89/155 (57%) Query: 52 LRFLGPVPASALLPLAFFFAPSSYAAAVFLIALATAFPVAVLTWSGVASVNKSYYDVART 111 ++ + P+P A +PLA + A+FLI+L FPV + T +GV V+ Y AR Sbjct: 116 VQVIRPIPPIAYIPLAILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARN 175 Query: 112 LGASEWFLILRVAIPAALPQVFVGLFMGLGAAFSVLVTAEMMGVKAGLGWYLQWSQGWAA 171 LGAS+ L LRV +PAA+P + G+ +G+G AF V++ AEM+ V GLG+ + ++ + Sbjct: 176 LGASQSTLFLRVMLPAAVPYILSGVRIGIGTAFIVVIVAEMIAVSNGLGFRIMEAREYFW 235 Query: 172 YSNMYAALIVMAILCSSLITLLFRVRDRALSWQKG 206 + A +I + +L ++ + R+ + L W +G Sbjct: 236 SDKIIAGMITIGLLGLAIDLGMSRLNNYMLRWHRG 270 >gi|300310541|ref|YP_003774633.1| nitrate transporter permease [Herbaspirillum seropedicae SmR1] Length = 307 Score = 75.9 bits (185), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 51/173 (29%), Positives = 89/173 (51%), Gaps = 5/173 (2%) Query: 3 SEDWSRLGISTAYSLRLLVHGFAAGGLVGFLVGATIGWSRVAGYWVHPVLRFLGPVPASA 62 S + +G + SL+ + GF LVG +G IG P++ L PV A Sbjct: 103 SPNDQGIGWNVLASLQRVGTGFGLAALVGIPLGFAIGRVEFLARMFGPIISLLRPVSPLA 162 Query: 63 LLPLAFFFAPSSYAAAVFLIALATAFPVAVLTWSGVASVNKSYYDVARTLGASEWFLILR 122 LP+ S+ AA++ I + + +P+ + T GV V + Y +VAR L SEW ++ + Sbjct: 163 WLPIGLLVFKSANPAAIWSIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKVLTK 222 Query: 123 VAIPAALPQVFVGLFMGLGAAFSVLVTAEMMGVKAGLGWYLQWSQGWAAYSNM 175 + P+ LP + G+ + +G A+ V+V AEM+ G+G+++ W ++N+ Sbjct: 223 ILFPSVLPYMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFWV-----WDEWNNL 270 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 70.1 bits (170), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 60/200 (30%), Positives = 101/200 (50%), Gaps = 12/200 (6%) Query: 4 EDWSRLGISTAYSLRLLVHGFAAGGLVGFLVGATIGWSRVAGYWVHPVLRFLGPVPASAL 63 E W L +S+ + GFA G +G ++G G R A + L+ + +PA AL Sbjct: 79 ELWRHLAVSSGRA----ASGFAVGAGLGLILGLLSGSLRSAELLLDTTLQMVRNIPALAL 134 Query: 64 LPLAFFFAPSSYAAAVFLIALATAFPVAVLTWSGVASVNKSYYDVARTLGASEWFLILRV 123 +PL + A +FL+++ FPV + T+ G+ SV++ ++A++ G S W L V Sbjct: 135 IPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWPLYRDV 194 Query: 124 AIPAALPQVFVGLFMGLGAAFSVLVTAEMMGVKAGLGWYLQWSQGWAAYSNMYAALIVMA 183 +P ALP + VGL LG + +L+ AE + +AG+G+ A + ++++ Sbjct: 195 ILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTM-----NAREFLQTDVVLVG 249 Query: 184 ILCSSLITLLFRVRDRALSW 203 IL L LL ++ D A W Sbjct: 250 IL---LYALLGKLADTAARW 266 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 47/150 (31%), Positives = 74/150 (49%) Query: 14 AYSLRLLVHGFAAGGLVGFLVGATIGWSRVAGYWVHPVLRFLGPVPASALLPLAFFFAPS 73 A SL + GF G + +G +G SR A + P + L +P+ A +PL + Sbjct: 77 AASLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPLLLLWLGI 136 Query: 74 SYAAAVFLIALATAFPVAVLTWSGVASVNKSYYDVARTLGASEWFLILRVAIPAALPQVF 133 V LIA+ FPV + +G+ ++ +V T G + LI + +PA+LP +F Sbjct: 137 DETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLPASLPYLF 196 Query: 134 VGLFMGLGAAFSVLVTAEMMGVKAGLGWYL 163 GL GL A+ LV AE++ G+G+ L Sbjct: 197 AGLRGGLSLAWMFLVAAELIAATKGVGYLL 226 >gi|300311644|ref|YP_003775736.1| taurine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 62.8 bits (151), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 39/131 (29%), Positives = 73/131 (55%), Gaps = 1/131 (0%) Query: 76 AAAVFLIALATAFPVAVLTWSGVASVNKSYYDVARTLGASEWFLILRVAIPAALPQVFVG 135 A+ + L+ LA PV + +GV S+ + + A +LGA+ W L+ V +P+ALP++ G Sbjct: 162 ASKITLLTLAMFAPVVLSAQAGVRSLPQERVNAALSLGATRWQLLTEVVLPSALPEILTG 221 Query: 136 LFMGLGAAFSVLVTAEMMGVKAGLGWYLQWSQGWAAYSNMYAALIVMAILCSSLITLLFR 195 + + LG +S LV AE++ G+G+ + + + A ++ + ++A C+ + R Sbjct: 222 IRIALGVGWSTLVAAELIASNRGIGYMIMSASHFLATDAVFVGIALIAT-CAFAFSYGMR 280 Query: 196 VRDRALSWQKG 206 + +RAL KG Sbjct: 281 LLERALVPWKG 291 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.138 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 531,665 Number of extensions: 18187 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 14 Length of query: 210 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 129 Effective length of database: 1,236,335 Effective search space: 159487215 Effective search space used: 159487215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)