BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0053.p (588 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312448|ref|YP_003776540.1| methyl-accepting chemotaxis tra... 676 0.0 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 386 e-108 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 378 e-106 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 372 e-104 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 370 e-103 >gi|300312448|ref|YP_003776540.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 596 Score = 676 bits (1744), Expect = 0.0, Method: Compositional matrix adjust. Identities = 372/521 (71%), Positives = 430/521 (82%) Query: 1 MRNITVRFSLMSALALFAAMIVFGAAVGVFALGRANESTVTVHDVASRALTINDAYKDTT 60 MRN+TVRFSLMSALALF+ MIV GA VG+FALGRAN +T VH++ RAL INDAYKDTT Sbjct: 1 MRNLTVRFSLMSALALFSCMIVVGAGVGIFALGRANHATEYVHEITERALLINDAYKDTT 60 Query: 61 RTRAALTRGYSALKEQNDQEVKNSALKSAQASYDRTLKLLDEFDKAAPFKGQDDKLKADL 120 RTRAA R Y+ L E+ DQ V +SALKSA+ +Y+R+LK LDE+ K +GQD K +L Sbjct: 61 RTRAAHGRMYATLMEKGDQAVIDSALKSAEMTYERSLKYLDEYAKLPDLEGQDPATKTEL 120 Query: 121 VNAGRQLSDVLNKAADALRANDTNAYVEINSRDITSRGANFSTQLEKFQKLTKDISEDIV 180 + + + L++VL KA D L+A D Y ++N+ I+ GA FST L+ +QK ++++++ Sbjct: 121 LESAKALNEVLKKATDLLKAGDAAGYSQLNNNFISKGGARFSTALDAYQKRAMELNDNVT 180 Query: 181 AQRDREYHMVVWLVAAGLVAALILVLGVHFLLRGIVLTPLNRAVALLDQVAGGDLTMKIS 240 QR REY++VVWLV GL+ A+ LV+GVHFLLR IVLTPLNRAV LLD VA GDLT K+ Sbjct: 181 TQRQREYNLVVWLVVTGLIGAMFLVVGVHFLLRNIVLTPLNRAVHLLDLVANGDLTTKVE 240 Query: 241 EPGRNEIGRLFASIRSMQQGLLSTVARVRSSTDIINTGAQEIAAGNLDLSSRTETQASSL 300 NEIGRLF++IRSMQQ LL+TV+RVRSS+D I+T A+EIAAGN+DLSSRTE QASSL Sbjct: 241 VASSNEIGRLFSAIRSMQQALLTTVSRVRSSSDSIDTSAKEIAAGNMDLSSRTEQQASSL 300 Query: 301 EETAASMEQLTGTVKQNAENAQQANQLAHSASETAVKGGEVVAQVVDTMTAINDSSKKIV 360 EETAASME+LTGTVKQNAENA QANQLAHSAS TA KGGEVV+QVVDTM AINDSS+KIV Sbjct: 301 EETAASMEELTGTVKQNAENALQANQLAHSASSTASKGGEVVSQVVDTMQAINDSSRKIV 360 Query: 361 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKQLIT 420 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIK LI Sbjct: 361 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKSLID 420 Query: 421 DSVDKVESGSRLVEQAGLTMGEVVGSVRRVTDIVGEISSASLEQSDGIAQVNRAIAQMDE 480 DSV+KVE+GS+LVEQAG TM EVV SV+RVTDIVGEI+ AS EQS GI QVNRAI QMDE Sbjct: 421 DSVEKVEAGSQLVEQAGATMSEVVTSVQRVTDIVGEIAEASREQSTGIEQVNRAITQMDE 480 Query: 481 VTQQNAALVEEAAAAAQSLQTQAVTLAEAVSIFRMEAHTAA 521 VTQQNAALVEEAAAAAQSLQ QA L AVSIF++EAH A Sbjct: 481 VTQQNAALVEEAAAAAQSLQAQATNLVGAVSIFKIEAHQVA 521 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 386 bits (991), Expect = e-108, Method: Compositional matrix adjust. Identities = 295/606 (48%), Positives = 371/606 (61%), Gaps = 77/606 (12%) Query: 17 FAAMIVFGAAVGVFALGRANESTVTVHDVASRALTINDAYKDTTRTRAALTRGYSALKE- 75 F ++ AA+GVF++ + ++ V S + I+ + + RT A L S L+ Sbjct: 17 FLVVVAMVAALGVFSIRQLDQ-------VNSASTEISTNWLPSIRTLARLQLAMSRLRSF 69 Query: 76 ----------------QNDQEVKNSALKSAQASYDRTLK------LLDEFDK-AAPFKGQ 112 + +V+ + LK+AQA Y + + E +K A F + Sbjct: 70 ELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVEKLIATFVSE 129 Query: 113 DDKLKADLVNAGRQLSDVLNKAADALRANDTNAYVEINSRDITSRGANFSTQLEKFQKL- 171 DK+ + RQ D +A + R T AY T L++ KL Sbjct: 130 HDKI----IALSRQGKD--EEARNLQRGESTKAY---------------RTSLQQIDKLA 168 Query: 172 -TKDISEDIV-AQRDREYHMV-VWL---VAAGLVAALILVLGVHFLLRGIVLTPLNRAVA 225 D DI Q D Y +W+ VAA + A++L + V L + PL AV+ Sbjct: 169 AVNDKGADISNKQADAIYAQARLWIIVTVAACIGLAMLLAVWVSRL----IARPLEEAVS 224 Query: 226 LLDQVAGGDLTMKISEPGRNEIGRLFASIRSMQQGLLSTVARVRSSTDIINTGAQEIAAG 285 + +VAGGDLT +I G++E G+L +++SM L+ V +VR T I T + EIA+G Sbjct: 225 VAQRVAGGDLTTRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASG 284 Query: 286 NLDLSSRTETQASSLEETAASMEQLTGTVKQNAENAQQANQLAHSASETAVKGGEVVAQV 345 NLDLSSRTE QASSLEETA++ME+LT TVKQNA+NA+QANQLA SASE A +GG VV QV Sbjct: 285 NLDLSSRTEEQASSLEETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQV 344 Query: 346 VDTMTAINDSSKKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLA 405 VDTM +IN SS+KI DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLA Sbjct: 345 VDTMGSINASSRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLA 404 Query: 406 QRSAAAAKEIKQLITDSVDKVESGSRLVEQAGLTMGEVVGSVRRVTDIVGEISSASLEQS 465 QRSA AAKEIK+LI SV +V+ GS+LVE+AG+TM EVV SVRRVTDIVGEISSAS EQS Sbjct: 405 QRSAGAAKEIKELIEGSVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQS 464 Query: 466 DGIAQVNRAIAQMDEVTQQNAALVEEAAAAAQSLQTQAVTLAEAVSIFRMEAHTAAAGPL 525 +GI QVN AIAQMDEVTQQNAALVEEAAAAAQSLQ QA TL + V F++E P Sbjct: 465 EGIEQVNLAIAQMDEVTQQNAALVEEAAAAAQSLQEQAETLTQTVGFFKLEQQ-----PD 519 Query: 526 HGSAVPTQRAAEKLTTVKKTAAVGAAAPAPAPSVGVRPALAKPATPKTAPKLPPTSDDD- 584 ++ P RAA +V +TAAV APA P+ A A PA P + +LP + +D Sbjct: 520 EPASRPPVRAA----SVARTAAV-RHAPATRPAA-TLAASAAPAKPASGSRLPGGAAEDG 573 Query: 585 --WEQF 588 WEQF Sbjct: 574 GSWEQF 579 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 378 bits (970), Expect = e-106, Method: Compositional matrix adjust. Identities = 262/523 (50%), Positives = 330/523 (63%), Gaps = 47/523 (8%) Query: 17 FAAMIVFGAAVGVFALGRANESTVTVHDVASRAL----------------TINDAYKDTT 60 FAA+IV +G+FA+ D+A+ L I++ T Sbjct: 17 FAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQASLPRMRISELQMVTA 76 Query: 61 RTRAALTRGYSALKEQNDQEVKNSALKSAQASYDRTLKLLDEFDKAAPFKGQDDKLKADL 120 T A A+K + D K A AQ S L ++F K+ + DK A + Sbjct: 77 ATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFSKSFATYLEIDKELARI 136 Query: 121 VNAGRQLSDVLNKAADALRANDTNAYVEINSRDITSRGANFSTQLEKFQKLTK------D 174 G++ + A AL D+N F + +E ++ K Sbjct: 137 SAEGKE------EEARALFKGDSNK--------------TFRSMIENMDEIIKVNDAGSK 176 Query: 175 ISEDIVAQRDREYHMVVWLVAAGLVAALILV-LGVHFLLRGIVLTPLNRAVALLDQVAGG 233 S+D+ Q R + W++ GL+AA++++ LG+ ++ V PLN AVA+ ++VA G Sbjct: 177 RSDDMAEQVYRSATL--WII--GLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQG 232 Query: 234 DLTMKISEPGRNEIGRLFASIRSMQQGLLSTVARVRSSTDIINTGAQEIAAGNLDLSSRT 293 DLTM I +E G L S+R+M L V VR+ TD I T +QEIA GNLDLSSRT Sbjct: 233 DLTMDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRT 292 Query: 294 ETQASSLEETAASMEQLTGTVKQNAENAQQANQLAHSASETAVKGGEVVAQVVDTMTAIN 353 E QA SLEETA++ME+LT TVKQNA+NA+QANQLA SAS+ A +GG VV QVV TM I Sbjct: 293 EEQAGSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGIT 352 Query: 354 DSSKKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAK 413 +SS +I DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AAK Sbjct: 353 ESSNRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAK 412 Query: 414 EIKQLITDSVDKVESGSRLVEQAGLTMGEVVGSVRRVTDIVGEISSASLEQSDGIAQVNR 473 EIKQLI DSV+KV +GS+LVE+AG TM EVV SVRRVTD+V EIS+AS EQS GI ++NR Sbjct: 413 EIKQLIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINR 472 Query: 474 AIAQMDEVTQQNAALVEEAAAAAQSLQTQAVTLAEAVSIFRME 516 AI QMDEVTQQNAALVEEAAAAAQSLQ QA L++ VS+F+++ Sbjct: 473 AITQMDEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKLQ 515 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 372 bits (954), Expect = e-104, Method: Compositional matrix adjust. Identities = 264/547 (48%), Positives = 347/547 (63%), Gaps = 66/547 (12%) Query: 1 MRN--ITVRFSL--MSALALFAAMIVFGAAVGVFALGRANESTVTVHDVASRALTINDAY 56 M+N I VR ++ ++ L L A M+ A+GV+ LG +I DA Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMV----ALGVWRLG-----------------SIGDAT 39 Query: 57 KDTTRTRAALTRGYSALKEQNDQEVKNSALKSAQASYDRTLKLLDEFDKA--APFKGQDD 114 D TR ALK++ D ++A+K RT ++ D A A F+ Q D Sbjct: 40 DDMTRI---------ALKKERDAVQWHAAIKENGV---RTFAVMKSDDDAFQAYFQKQID 87 Query: 115 -----------KLKADLVNAG-RQLSDVLNKAADALRANDTNAY-VEINSRDITSRGANF 161 +++A + +A ++L D + K A RA + + V+ D +R Sbjct: 88 AQSARISQLQKEVEAAITDAEEKRLFDEVGKLRAAYRATRQSIFDVKTAGDDARAREMTD 147 Query: 162 STQLE----------KFQKLTKDISEDIVAQRDREYHMVVWLVAAGLVAALILVLG--VH 209 +T ++ ++ K + + A +R+Y ++ A + A+ +VLG + Sbjct: 148 TTLVKMMDDYADGVLRYADFQKQVIDQTAAAINRDYRSGRSMLLA--LGAIEIVLGAALA 205 Query: 210 FLLRGIVLTPLNRAVALLDQVAGGDLTMKISEPGRNEIGRLFASIRSMQQGLLSTVARVR 269 +LL + PLN+AV + + VA GDLT +I R+E G+L A++++M LL V RVR Sbjct: 206 WLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRDETGKLLAALKTMNGNLLDIVGRVR 265 Query: 270 SSTDIINTGAQEIAAGNLDLSSRTETQASSLEETAASMEQLTGTVKQNAENAQQANQLAH 329 T+ INT ++EIA GNLDLS+RTE QA SLEETA++MEQLT TVKQNA+NA+QAN+LA Sbjct: 266 MGTETINTASREIATGNLDLSARTEAQAGSLEETASAMEQLTSTVKQNADNARQANELAA 325 Query: 330 SASETAVKGGEVVAQVVDTMTAINDSSKKIVDIISVIDGIAFQTNILALNAAVEAARAGE 389 SAS+ A +GG VV QVV TM AI+ SS+KI DII VIDGIAFQTNILALNAAVEAARAGE Sbjct: 326 SASQVATQGGAVVDQVVATMGAIHASSQKIADIIGVIDGIAFQTNILALNAAVEAARAGE 385 Query: 390 QGRGFAVVASEVRSLAQRSAAAAKEIKQLITDSVDKVESGSRLVEQAGLTMGEVVGSVRR 449 QGRGFAVVASEVRSLAQRSA+AAKEIKQLI DSV+KV+ GSRLV +AG TM EVV SV R Sbjct: 386 QGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKVDDGSRLVARAGDTMQEVVQSVAR 445 Query: 450 VTDIVGEISSASLEQSDGIAQVNRAIAQMDEVTQQNAALVEEAAAAAQSLQTQAVTLAEA 509 VT +V EI+ AS EQSDGI QVN AI +DE TQQNAALVE+AAAAA+S+Q QA +L +A Sbjct: 446 VTAVVREITLASQEQSDGIEQVNLAITHIDETTQQNAALVEQAAAAARSMQDQAASLTDA 505 Query: 510 VSIFRME 516 VS+FR+E Sbjct: 506 VSVFRLE 512 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 370 bits (949), Expect = e-103, Method: Compositional matrix adjust. Identities = 238/410 (58%), Positives = 286/410 (69%), Gaps = 37/410 (9%) Query: 191 VWLVAAGLVAALILVLGVHFLLRGIVLTPLNRAVALLDQVAGGDLTMKISEPGRNEIGRL 250 + LV +G VA L+ L + R I PLN AV + VA GDLT++I ++E G L Sbjct: 192 IMLVLSG-VAVLLSALCAMLITRSIT-RPLNEAVQVATAVAAGDLTVQIEATSKDETGAL 249 Query: 251 FASIRSMQQGLLSTVARVRSSTDIINTGAQEIAAGNLDLSSRTETQASSLEETAASMEQL 310 +++SM L VA VR+ TD INT ++EIA GNLDLSSRTE QA +LEETA++ME+L Sbjct: 250 LGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALEETASAMEEL 309 Query: 311 TGTVKQNAENAQQANQLAHSASETAVKGGEVVAQVVDTMTAINDSSKKIVDIISVIDGIA 370 T TV+QNA+NA+QAN LA +AS+ A++GG VV +VV TM IND+S+KIVDIISVIDGIA Sbjct: 310 TSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVDIISVIDGIA 369 Query: 371 FQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKQLITDSVDKVESGS 430 FQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSAAAAKEIK LI SV++VESGS Sbjct: 370 FQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLIDASVERVESGS 429 Query: 431 RLVEQAGLTMGEVVGSVRRVTDIVGEISSASLEQSDGIAQVNRAIAQMDEVTQQNAALVE 490 RLV QAG TM EVV SV+RVTD+V EI++AS EQSDGI Q+N+AI QMDEVTQQNAALVE Sbjct: 430 RLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQAIVQMDEVTQQNAALVE 489 Query: 491 EAAAAAQSLQTQAVTLAEAVSIFRM-EAHTAAAGPLHGSAVPTQRAAEKLTTVKKTAAVG 549 +AAAAAQSLQ Q+ L++ V IFR+ H PL + P QR+ + Sbjct: 490 QAAAAAQSLQEQSGRLSDTVGIFRLGNDHAHQPSPL---SAPVQRSVQ------------ 534 Query: 550 AAAPAPAPSVGVRPALA-KPATPKTAP----------KLPPTSDDDWEQF 588 APA RP LA KP T +AP +D WEQF Sbjct: 535 -VAPA-------RPVLARKPVTRTSAPAKSIPAAQPVAQAALADGQWEQF 576 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.125 0.327 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,091,154 Number of extensions: 33272 Number of successful extensions: 453 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 71 Length of query: 588 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 498 Effective length of database: 1,193,720 Effective search space: 594472560 Effective search space used: 594472560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)