BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0051.p (556 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 466 e-132 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 463 e-131 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 432 e-122 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 415 e-117 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 406 e-114 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 466 bits (1200), Expect = e-132, Method: Compositional matrix adjust. Identities = 291/550 (52%), Positives = 375/550 (68%), Gaps = 7/550 (1%) Query: 1 MKISNLNIGVRLSLAFALVVALLIGTALVGIRHLDANNEKMDRIVGERYSLISISNQIKN 60 MKIS++ I VRL FA ++ ++ A VG+++L ++ D IV +RY +++ QI + Sbjct: 2 MKISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQITD 61 Query: 61 NGNKGNAILSNLLLTSSPERAKKYMDDYAALRKTNAEAYAKFEKMLQNEQSKQLFKQQFE 120 N G L N ++ +PE KKY+D +E A+ EK + +L + Sbjct: 62 KVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGSELLAALKQ 121 Query: 121 SRAAYGVTVRKFFELIAENKQQEARDIYQGDMARTQAAYYVLVDKMVDYQAHEMENDVLN 180 +RA Y K +LI + K+ EA Q Y+ V +MV +Q+ M+ Sbjct: 122 ARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKSAER 181 Query: 181 AAEQARNAKIQMAILSACAVLLSIVTGVFITRTITRPINRAVDLAEAVAQGNLTHHLEID 240 A+ + +A M +LS AVLLS + + ITR+ITRP+N AV +A AVA G+LT +E Sbjct: 182 ASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIEAT 241 Query: 241 SKDEIGRLLVALKFMIENLHGIVAKVRKGTDTIAEASREVASGNMDLSTRTEQQASSLEE 300 SKDE G LL ALK M +NLH IVA+VR GTDTI ASRE+A+GN+DLS+RTEQQA +LEE Sbjct: 242 SKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALEE 301 Query: 301 TASAMEQLTSAVRQNADNARQANQLASNASAVAIKGGDAVGQVVDTMTAINTSSRKIVDI 360 TASAME+LTS VRQNADNARQAN LA AS VAI+GG VG+VV TM IN +SRKIVDI Sbjct: 302 TASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVDI 361 Query: 361 IGVIDGIAFQTNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHS 420 I VIDGIAFQTNILALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIK+LID S Sbjct: 362 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLIDAS 421 Query: 421 VNQVGNGSKMVEQAGQTIQDVVNSIRHVTDIVAEISSSSQEQSAGIEQINQAITQMDQVT 480 V +V +GS++V QAG T+++VV S++ VTD+VAEI+++S EQS GIEQINQAI QMD+VT Sbjct: 422 VERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQAIVQMDEVT 481 Query: 481 QENAALVEQSAAAAQALQDQTGQLTDMVSTFTLHGDQL--PATATAPGGTSRRSVDITAK 538 Q+NAALVEQ+AAAAQ+LQ+Q+G+L+D V F L D P+ +AP +RSV + Sbjct: 482 QQNAALVEQAAAAAQSLQEQSGRLSDTVGIFRLGNDHAHQPSPLSAP---VQRSVQVA-- 536 Query: 539 PSRLGAVKTP 548 P+R + P Sbjct: 537 PARPVLARKP 546 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 463 bits (1192), Expect = e-131, Method: Compositional matrix adjust. Identities = 277/513 (53%), Positives = 368/513 (71%) Query: 1 MKISNLNIGVRLSLAFALVVALLIGTALVGIRHLDANNEKMDRIVGERYSLISISNQIKN 60 MK+ ++ I VRL++ FA+++ ++ VG++ L + ++ IV +RY I+++ +I++ Sbjct: 1 MKLKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRD 60 Query: 61 NGNKGNAILSNLLLTSSPERAKKYMDDYAALRKTNAEAYAKFEKMLQNEQSKQLFKQQFE 120 N N L N LL + E A +Y+D AA EA K EK+L + ++LF+ E Sbjct: 61 NVNGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQE 120 Query: 121 SRAAYGVTVRKFFELIAENKQQEARDIYQGDMARTQAAYYVLVDKMVDYQAHEMENDVLN 180 +R+AY K ELI + K++EA ++ + Q Y+ ++D+ V +Q ME V Sbjct: 121 ARSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTE 180 Query: 181 AAEQARNAKIQMAILSACAVLLSIVTGVFITRTITRPINRAVDLAEAVAQGNLTHHLEID 240 + ++A M LSA A+ L ++ +TR+ITRP+N AV +A AVA G+LT + Sbjct: 181 GRDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGAT 240 Query: 241 SKDEIGRLLVALKFMIENLHGIVAKVRKGTDTIAEASREVASGNMDLSTRTEQQASSLEE 300 SKDE G LL ALK M ++LH IV++VR+G+DTI AS E+A+GN+DLS+RTE+QA +LEE Sbjct: 241 SKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEE 300 Query: 301 TASAMEQLTSAVRQNADNARQANQLASNASAVAIKGGDAVGQVVDTMTAINTSSRKIVDI 360 TASAME+LTS V+QNADNARQAN LA+ AS VA++GG VGQVV TM IN +SRKIVDI Sbjct: 301 TASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDI 360 Query: 361 IGVIDGIAFQTNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHS 420 I VIDGIAFQTNILALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIK LID S Sbjct: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALIDDS 420 Query: 421 VNQVGNGSKMVEQAGQTIQDVVNSIRHVTDIVAEISSSSQEQSAGIEQINQAITQMDQVT 480 V++V NGS++VEQAG T+ +VV S+R VTD+VAEIS++S EQS GIEQIN AITQMDQVT Sbjct: 421 VSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQVT 480 Query: 481 QENAALVEQSAAAAQALQDQTGQLTDMVSTFTL 513 Q+NAALVE++AAAAQ+LQ+Q+ +L+ VS F L Sbjct: 481 QQNAALVEEAAAAAQSLQEQSTRLSATVSVFKL 513 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 432 bits (1111), Expect = e-122, Method: Compositional matrix adjust. Identities = 274/548 (50%), Positives = 360/548 (65%), Gaps = 31/548 (5%) Query: 3 ISNLNIGVRLSLAFALVVALLIGTALVGIRHLD----------ANNEKMDRIVGERYSLI 52 I N+NIG RL+L FAL++ I A +G+ LD A K +R++ + Y+ Sbjct: 4 IGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYT-- 61 Query: 53 SISNQIKNNGNKGNAILSNLLLTSSPERAKKYMDDYAALRKTNAEAYAKFEKMLQNEQSK 112 N G + +S P + + AA K++ E + E +L + K Sbjct: 62 --------NLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEK 113 Query: 113 QLFKQQFESRAAYGVTVRKFFELIAENKQQEARDIYQGDMARTQAAYYVLVDKMVDYQAH 172 LF++ E R Y + + + A +EA I AY + K+VD+Q Sbjct: 114 ALFQKIGELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRK 173 Query: 173 EMENDVLNAAEQARNAKIQMAILSACAVLLSIVTGVFITRTITRPINRAVDLAEAVAQGN 232 EM++ A ++ + +L +LL IV ++T IT+P++RAVD++ VA+G+ Sbjct: 174 EMDDTAREIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGD 233 Query: 233 LTHHLEIDSKDEIGRLLVALKFMIENLHGIVAKVRKGTDTIAEASREVASGNMDLSTRTE 292 L+ + + S+DE G+LL +LK M ++L GIV+ VR GTDTI+ AS E+A+GN+DLS+RTE Sbjct: 234 LSSEIAVTSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTE 293 Query: 293 QQASSLEETASAMEQLTSAVRQNADNARQANQLASNASAVAIKGGDAVGQVVDTMTAINT 352 QQASSL ETASAME+L S VRQNADNARQA+QLA++ASAVA +GG V QVVDTM AIN Sbjct: 294 QQASSLAETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINA 353 Query: 353 SSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKE 412 SSRKIVDII VIDGIAFQTNILALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKE Sbjct: 354 SSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKE 413 Query: 413 IKTLIDHSVNQVGNGSKMVEQAGQTIQDVVNSIRHVTDIVAEISSSSQEQSAGIEQINQA 472 IK LI+ SV +V +GS++VEQAG T+Q+VV+S+R VTDIVAEIS++S EQ+ GIEQIN A Sbjct: 414 IKELINDSVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLA 473 Query: 473 ITQMDQVTQENAALVEQSAAAAQALQDQTGQLTDMVSTFTLHGDQLPATATAPGGTSRRS 532 ITQMDQVTQ+NAALVE++AAAA ++Q Q G+L MVS F L ++ P RR Sbjct: 474 ITQMDQVTQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLS-EREPLV--------RRE 524 Query: 533 VDITAKPS 540 +D+T PS Sbjct: 525 IDVT--PS 530 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 415 bits (1067), Expect = e-117, Method: Compositional matrix adjust. Identities = 256/536 (47%), Positives = 353/536 (65%), Gaps = 3/536 (0%) Query: 1 MKISNLNIGVRLSLAFALVVALLIGTALVGIRHLDANNEKMDRIVGERYSLISISNQIKN 60 M+ +NL+I RL++AF++++ ++ G +G+ L N+ +D +V +RY I+I N I Sbjct: 1 MRFNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISG 60 Query: 61 NGNKGNAILSNLLLTSSPERAKKYMDDYAALRKTNAEAYAKFEKMLQNEQSKQLFKQQFE 120 + L N LL + P + +L N + Y + +++ N ++K + E Sbjct: 61 KVDDVAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTE 120 Query: 121 SRAAYGVTVRKFFELIAENKQQEARDIYQGDMARTQAAYYVLVDKMVDYQAHEMENDVLN 180 +R +Y ++ +L + + A + +M Q Y+ +D++ D Q M+ V Sbjct: 121 TRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQ 180 Query: 181 AAEQARNAKIQMAILSACAVLLSIVTGVFITRTITRPINRAVDLAEAVAQGNLTHHLEID 240 A ++ M I A + I+R+ITRP++RAV++AE VA G+LT + Sbjct: 181 AEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAAH 240 Query: 241 SKDEIGRLLVALKFMIENLHGIVAKVRKGTDTIAEASREVASGNMDLSTRTEQQASSLEE 300 S DE GRLL AL M + L IV +VR+GTD++ AS ++A+GN+DLS+RTE+QAS+LEE Sbjct: 241 SNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALEE 300 Query: 301 TASAMEQLTSAVRQNADNARQANQLASNASAVAIKGGDAVGQVVDTMTAINTSSRKIVDI 360 TAS++E+LTS+V+QNAD+ R++++LA+NAS VA +GG AV QVV TM AIN SS KIVDI Sbjct: 301 TASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVDI 360 Query: 361 IGVIDGIAFQTNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHS 420 I VIDGIAFQTNILALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIK LI+ S Sbjct: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKELINDS 420 Query: 421 VNQVGNGSKMVEQAGQTIQDVVNSIRHVTDIVAEISSSSQEQSAGIEQINQAITQMDQVT 480 VNQV +G+++V QAG T+ VVNSI V+ IV EIS++++EQS GIEQ+NQAI QMD T Sbjct: 421 VNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQMDNTT 480 Query: 481 QENAALVEQSAAAAQALQDQTGQLTDMVSTFTLH--GDQLPA-TATAPGGTSRRSV 533 Q+NAALVE++AAAAQALQDQ +L D+V F L G PA +A AP R V Sbjct: 481 QQNAALVEEAAAAAQALQDQAQRLLDLVGVFRLDEVGGSGPAPSAVAPVAPVLRKV 536 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 406 bits (1043), Expect = e-114, Method: Compositional matrix adjust. Identities = 251/513 (48%), Positives = 342/513 (66%), Gaps = 5/513 (0%) Query: 5 NLNIGVRLSLAFALVVALLIGTALVGIRHLDANNEKMDRIVGERYSLISISNQIKNNGNK 64 NL + VRL+ FA +VALL ++ ++ N +D+IV +RY + + +++ N+ Sbjct: 7 NLKVAVRLAAVFAFIVALLAVVSVTAFIKINNINRAIDQIVNDRYLKVRWAFDLRDGVNE 66 Query: 65 GNAILSNLLL--TSSPERAKKYMDDYAALRKTNAEAYAKFEKMLQNEQSKQLFKQQFESR 122 L + + + P K++ A+++T EA K ++ K E+R Sbjct: 67 QIKYLRGIAIDIENRPANEKRFAQLEVAVKQTK-EALDKITARQVTAVGQKKAKALDEAR 125 Query: 123 AAYGVTVRKFFELIAENKQQEARDIYQGDMARTQAAYYVLVDKMVDYQAHEMENDVLNA- 181 A+ + F L+ A + + TQ Y L D Q +++ + A Sbjct: 126 IAFDASKADFLALVKAGNSTSADEYVLRKITDTQNNYLALARAFADSQDAQLQAEGAKAV 185 Query: 182 AEQARNAKIQMAILSACAVLLSIVTGVFITRTITRPINRAVDLAEAVAQGNLTHHLEIDS 241 A+ A ++ +A S AVL +I+ G + R+I +P+ AV +AE VA G+LT +E S Sbjct: 186 ADGAMAIEVTLA-CSMAAVLAAIILGYLMARSIVKPLTEAVRVAENVAAGDLTTRIEPHS 244 Query: 242 KDEIGRLLVALKFMIENLHGIVAKVRKGTDTIAEASREVASGNMDLSTRTEQQASSLEET 301 +DE G+L+ AL+ M +NL IV+ VR+GT++I AS E+ASGN+DLS RTEQQA SLEET Sbjct: 245 RDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEET 304 Query: 302 ASAMEQLTSAVRQNADNARQANQLASNASAVAIKGGDAVGQVVDTMTAINTSSRKIVDII 361 ASAME++TS VRQNADNARQANQ+A +AS +A++GG VG+VV TM IN SSRKIVDII Sbjct: 305 ASAMEEMTSTVRQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDEINQSSRKIVDII 364 Query: 362 GVIDGIAFQTNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHSV 421 GVIDGIAFQTNILALNAAVEAARAGE GRGFAVVA+EVR+LAQRSA AAKEIK LI SV Sbjct: 365 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKALIADSV 424 Query: 422 NQVGNGSKMVEQAGQTIQDVVNSIRHVTDIVAEISSSSQEQSAGIEQINQAITQMDQVTQ 481 +V GS +V QAG T++ VV+S+R VTDIVAEIS++S EQS+GIE+IN+A+TQMD+ TQ Sbjct: 425 GKVEGGSALVAQAGATMEQVVSSVRSVTDIVAEISAASAEQSSGIEEINRAVTQMDEGTQ 484 Query: 482 ENAALVEQSAAAAQALQDQTGQLTDMVSTFTLH 514 +NAALVEQ+AAAA+++Q+Q +LT +S F L Sbjct: 485 QNAALVEQAAAAAKSMQEQAVRLTQSISIFKLE 517 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.126 0.326 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,148,418 Number of extensions: 37006 Number of successful extensions: 462 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 222 Number of HSP's successfully gapped: 71 Length of query: 556 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 466 Effective length of database: 1,193,720 Effective search space: 556273520 Effective search space used: 556273520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)