BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0050.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 118 3e-28 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 108 2e-25 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 99 1e-22 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 97 1e-21 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 95 3e-21 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 118 bits (295), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 67/167 (40%), Positives = 97/167 (58%), Gaps = 4/167 (2%) Query: 3 GLKIGHALTADTNIGPVVDQSQLDQNLSYIEIGKQEGANLVFGGERVKRDTDGFYMTPAL 62 G+ +G +GPV ++Q ++ YI+ G EGA L+ GG V R G+++ P + Sbjct: 315 GITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG--VSRPDKGWFVQPTI 372 Query: 63 FADCNNDMRISREEIFGPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHFKRH 122 FAD NN M I+REEIFGPV ++I D E A+ LAND+++GL++ + TT+L A Sbjct: 373 FADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAG 432 Query: 123 AQAGMVMVNTATAGVDYHVPFGGRKASSYGPREQGAYAAEFYTAVKT 169 + G V VN + +D ++P+GG K S G RE G A YT KT Sbjct: 433 VKVGAVGVNCWSP-LDANLPWGGIKDSGMG-REGGLAGALAYTEEKT 477 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 108 bits (271), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 72/171 (42%), Positives = 91/171 (53%), Gaps = 6/171 (3%) Query: 1 LAGLKIGHALTADTNIGPVVDQSQLDQNLSYIEIGKQEGANLVFGGERVKRDTD---GFY 57 +A +K G+ L T IG Q QL++ LSYI+IGKQEGA ++ GG R + D G+Y Sbjct: 325 VAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYY 384 Query: 58 MTPALFADCNNDMRISREEIFGPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHAT 117 + P +F NN MRI +EEIFGPV SV + E AL +ANDT +GL +G+ T A Sbjct: 385 VKPTVF-QGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAF 443 Query: 118 HFKRHAQAGMVMVNTATAGVDYHVPFGGRKASSYGPREQGAYAAEFYTAVK 168 R QAG V N H FGG K S G RE + Y K Sbjct: 444 RMGREIQAGRVWTNCYHL-YPAHAAFGGYKQSGIG-RENHKMMLDHYQQTK 492 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 99.4 bits (246), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 62/169 (36%), Positives = 88/169 (52%), Gaps = 4/169 (2%) Query: 1 LAGLKIGHALTADTNIGPVVDQSQLDQNLSYIEIGKQEGANLVFGGE-RVKRDTDGFYMT 59 LA +K+G A T GP+ +++Q + IE G Q+GA L+ GG R G+Y Sbjct: 314 LAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYAR 373 Query: 60 PALFADCNNDMRISREEIFGPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHF 119 P +F++ + DM I++EEIFGPV +++P D E A+ +ANDT +GL + + Sbjct: 374 PTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAV 433 Query: 120 KRHAQAGMVMVNTATAGVDYHVPFGGRKASSYGPREQGAYAAEFYTAVK 168 Q+G V +N D PFGG K S G RE G E Y VK Sbjct: 434 AARIQSGQVHLN--YPAWDPQAPFGGYKQSGNG-REYGVEGMEEYMEVK 479 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 96.7 bits (239), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 56/169 (33%), Positives = 86/169 (50%), Gaps = 4/169 (2%) Query: 4 LKIGHALTADTNIGPVVDQSQLDQNLSYIEIGKQEGANLVFGGERVKRDTDGFYMTPALF 63 +++G L T +GP+ ++ Q + + G + GA L G R + G+++ P L Sbjct: 326 IRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYGEE--GYFVRPTLL 383 Query: 64 ADCNNDMRISREEIFGPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHFKRHA 123 A +N M ++R EIFGPVA IP ++ E A+ +AND+EFGL+ + T + A Sbjct: 384 AHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVAASV 443 Query: 124 QAGMVMVNTATAGVDYHVPFGGRKASSYGPREQGAYAAEFYTAVKTSYT 172 AG VN+ ++ PFGG S YG R G A YT K+ + Sbjct: 444 NAGTFWVNSYKT-INVASPFGGFNRSGYG-RSSGMEALYDYTQTKSVWV 490 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 94.7 bits (234), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 55/167 (32%), Positives = 92/167 (55%), Gaps = 3/167 (1%) Query: 4 LKIGHALTADTNIGPVVDQSQLDQNLSYIEIGKQEGANLVFGGE-RVKRDTDGFYMTPAL 62 L++G + D + GP++ ++Q ++ ++ +++G L G+ T GFY PAL Sbjct: 307 LQVGSS-QMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFYQAPAL 365 Query: 63 FADCNNDMRISREEIFGPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHFKRH 122 D R+++EE+FGPV + +P ++ HA+ LAN T++GL + + T R Sbjct: 366 LRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQMRLARA 425 Query: 123 AQAGMVMVNTATAGVDYHVPFGGRKASSYGPREQGAYAAEFYTAVKT 169 ++G V +N AG +PFGG KAS +G RE+G A +T +KT Sbjct: 426 IRSGQVFINNYGAGGGVELPFGGVKASGHG-REKGFEALYGFTVLKT 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 549,687 Number of extensions: 21934 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 54 Number of HSP's successfully gapped: 16 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)