BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0005.p (243 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313790|ref|YP_003777882.1| permease [Herbaspirillum serope... 203 1e-53 >gi|300313790|ref|YP_003777882.1| permease [Herbaspirillum seropedicae SmR1] Length = 253 Score = 203 bits (516), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 130/238 (54%), Positives = 165/238 (69%), Gaps = 7/238 (2%) Query: 4 LNSPDRPSAIRYLN----RVTCQGIAMSFLAGYVDTLGFVALFGLFTAHVTGNFVLIGSE 59 L S D +A +N R Q ++FLA YVDTLGF+ALFGLFTAHVTGNF+LIG+ Sbjct: 8 LESNDALTASPPMNAAAQRNRLQSSTLAFLAAYVDTLGFIALFGLFTAHVTGNFILIGAA 67 Query: 60 LANPSH-GVLIKFLAFPAFILSVVMTRLVVLALERSGKPALPWVLMLQLIFLLAFMGFGQ 118 LA+ S +L+KFLAFPAFIL V RL + A++R AL W L+LQL+ LL FM G Sbjct: 68 LADSSQMSILLKFLAFPAFILGVAAARLFIAAIQRRQGRALGWSLLLQLVLLLGFMVCGV 127 Query: 119 SIAPVGVADARATAALLAGMFGAAAMGIQNAAARLILPQAAPTTVMTGNVTQLVIDVTDI 178 + APV A + AG+ G A+MG+ +A +RL+L APT++MTGNVTQ+VIDV D+ Sbjct: 128 AAAPV--QSPTAGWVMAAGLLGTASMGVHSAISRLLLAHLAPTSLMTGNVTQVVIDVVDM 185 Query: 179 VRGAADDTVRQRCVKFFWPIVAFGVGAIGGAFAYHQALFFALLLPVFILIAMIVAEFR 236 +RGAAD +RCVKF WP+++FGVGAI AFAYH F L+ PV IL+A+IVAEFR Sbjct: 186 LRGAADAGTTERCVKFVWPVLSFGVGAIAAAFAYHAIGFAGLIAPVLILVALIVAEFR 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.141 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 568,207 Number of extensions: 17954 Number of successful extensions: 90 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 1 Length of query: 243 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 160 Effective length of database: 1,226,865 Effective search space: 196298400 Effective search space used: 196298400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 129 (54.3 bits)