BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0078.p (333 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspir... 583 e-168 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 80 3e-16 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 79 5e-16 gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 79 8e-16 gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 74 2e-14 >gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspirillum seropedicae SmR1] Length = 632 Score = 583 bits (1502), Expect = e-168, Method: Compositional matrix adjust. Identities = 281/330 (85%), Positives = 305/330 (92%) Query: 1 VRRFINAPDIDEVIFVRGTTEAINLVAKSWGAQHVGEGDEIIVSNLEHHANIVPWQQLAA 60 VR F+NAPD++EVIFVRGTTEAINLVAKSWG +HVG GDEIIVS+LEHHANIVPWQQLAA Sbjct: 300 VRAFLNAPDVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAA 359 Query: 61 AKGAKLRVIPVDDSGQILLDEYRKLLNDRTRIVAITQVSNALGTVTPVKEIVELAHRAGA 120 KGAKLRVIPVDDSGQ+LLDEY KLLN+RTRIVA+TQVSNALGT+TP K+IVELA AGA Sbjct: 360 EKGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGA 419 Query: 121 KALVDGAQSVSHMRVDVQELGADFFVFSGHKVFGPTGIGVVWGKRDVLEDMPPWQGGGNM 180 + LVDGAQSVSHMR DVQ LGADFFVFSGHKVFGPTGIG +WG+RDVLEDMPPWQGGGNM Sbjct: 420 RTLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRDVLEDMPPWQGGGNM 479 Query: 181 IADVTFEKTVFQPIPNKFEAGTGNIADAVGLGAAIDYVNKIGIENIARHEHDLLVYGMQQ 240 IADVTFEKT+FQP+PN FEAGTGNIADAVGLGAAIDYVN++GI+NIA +EH LL YGM Q Sbjct: 480 IADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQ 539 Query: 241 LREIKGVRLIGTADDKASVMSFVLAGYSTEEVGHALNEEGIAVRTGHHCAQPILRRFGVE 300 L EI+G+RLIGTA KASVMSFVL GYSTE+VG ALN+EGIAVRTGHHCAQPILRRFGVE Sbjct: 540 LGEIEGLRLIGTAAHKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQPILRRFGVE 599 Query: 301 TTVRPSLAFYNTFDEIDRLATVVRRLAGQR 330 TTVRPSLAFYNTF+EID L TVVRRLA QR Sbjct: 600 TTVRPSLAFYNTFEEIDCLLTVVRRLAAQR 629 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 83/345 (24%), Positives = 147/345 (42%), Gaps = 35/345 (10%) Query: 1 VRRFINAPDIDEVIFVRGTTEAINLVAKSWGAQHVGEGDEIIVSNLEHHANIVPWQQLAA 60 V + +NA D E+I+ G TE+ NL K + +G II EH A + +++L Sbjct: 71 VAKLVNA-DSREIIWTSGATESNNLAIKGAAQFYKSKGKHIITVKTEHKAVLDTFRELER 129 Query: 61 AKGAKLRVIPVDDSGQILLDEYRKLLNDRTRIVAITQVSNALGTVTPVKEIVELAHRAGA 120 G + + D+G + +++ + T +V++ V+N +G + PV EI E G Sbjct: 130 I-GFEATYLQPQDNGLVTVEQIAAAVRPDTILVSVMLVNNEIGVIQPVPEIGEFCRSKGI 188 Query: 121 KALVDGAQSVSHMRVDVQELGADFFVFSGHKVFGPTGIGVVWGKRDVLEDMPPWQGGGNM 180 D AQ+ +++D++ D FS HK +GP GIG ++ +R + GG Sbjct: 189 IFHSDAAQATGKVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVRIEAQMHGGGH 248 Query: 181 IADVTFEKTVFQPIPNKFEAGTGNIADAVGLGAAIDYVNKIGIENIARHE--HDLLVYGM 238 +GT VG+G A + +AR D L G+ Sbjct: 249 --------------ERGLRSGTLATHQIVGMGEAFRLAREEMDSELARIRALRDRLAKGL 294 Query: 239 QQLREIKGVRLIGTADDKASVMSFVLAGY-STEEVGHALNEEGIAVRTGHHCA----QP- 292 Q++ E + G + + V Y E + A+ + IAV +G C +P Sbjct: 295 QEIEE---TYINGDMEHRVPHNLNVSFNYVEGESLIMAIKD--IAVSSGSACTSASLEPS 349 Query: 293 -ILRRFG-----VETTVRPSLAFYNTFDEIDRLATVVRRLAGQRR 331 +LR G +++R ++ + T ++ID +++ G+ R Sbjct: 350 YVLRALGRSDELAHSSIRFTIGRFTTEEDIDFTIALIKSKVGKLR 394 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 79.0 bits (193), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 85/356 (23%), Positives = 146/356 (41%), Gaps = 61/356 (17%) Query: 4 FINAPDIDEVIFVRGTTEAINLVAKSWGAQHVGEGDEIIVSNLEHHANIVPWQQLAAAKG 63 +NA D+ E+I+ G TE+ NL K +G +I EH A + ++L +G Sbjct: 63 LVNA-DVREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELER-RG 120 Query: 64 AKLRVIPVDDSGQILLDEYRKLLNDRTRIVAITQVSNALGTVTPVKEIVELAHRAGAKAL 123 ++ + V +G + L + L T + ++ V+N +G + + + ++ G Sbjct: 121 FEVTYLEVGSNGLLDLAAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLH 180 Query: 124 VDGAQSVSHMRVDVQELGADFFVFSGHKVFGPTGIGVVW-GKRDVLEDMPPWQGGGNMIA 182 VD AQ+ + +D+ L D FS HK +GP GIG ++ G++ + GGG+ Sbjct: 181 VDAAQATGKVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGH--- 237 Query: 183 DVTFEKTVFQPIPNKFEAGTGNIADAVGLGAAIDYVN-KIGIENIARHEHDLLVYGMQQL 241 +GT VG+G A + +G EN +++ Sbjct: 238 ------------ERGLRSGTLATHQIVGMGEAFELARLAMGTEN-------------ERI 272 Query: 242 REIKGVRLIGTADDKASVMSFVLAGYSTEEVGHALN-----EEG---------IAVRTGH 287 R ++ G AD + + G V H LN EG +AV +G Sbjct: 273 RMLRDRLHAGFAD----IEECFVNGDLERRVPHNLNVSFNYVEGESLLMAIKDVAVSSGS 328 Query: 288 HCAQP------ILRRFGVE-----TTVRPSLAFYNTFDEIDRLATVVRRLAGQRRA 332 C +LR G+ +++R S+ + T +EID V+R G+ RA Sbjct: 329 ACTSASLEPSYVLRALGMSDELAHSSIRFSIGRFTTVEEIDFTVNVIREKVGKLRA 384 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 78.6 bits (192), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 50/159 (31%), Positives = 79/159 (49%), Gaps = 4/159 (2%) Query: 15 FVRGTTEAINLVAKSWGAQHVGEGDEIIVSNLEHHANIVPWQQLAAAKGAKLRVIPVDDS 74 F G TEA ++ GA + IVS+L H + + + A+G ++ I VD+ Sbjct: 68 FTSGATEANHMAI--LGALALQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWIAVDEH 125 Query: 75 GQILLDEYRKLLNDRTRIVAITQVSNALGTVTPVKEIVELAHRAGAKALVDGAQSVSHMR 134 GQ+ LDE + T +V++ +N G V P+ + ELA + G D Q+V + Sbjct: 126 GQLQLDELAAAITPDTALVSLMWANNETGVVFPIAQAAELARQRGVLFHTDAVQAVGRVP 185 Query: 135 VDVQELGADFFVFSGHKVFGPTGIGVVWGKRDVLEDMPP 173 DV LG D S HK+ GP GIG ++ ++ + +PP Sbjct: 186 CDVAGLGVDLLSLSAHKLHGPKGIGALYVRKGI--KLPP 222 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 88/332 (26%), Positives = 139/332 (41%), Gaps = 38/332 (11%) Query: 12 EVIFVRGTTEAINLVAK--SWGAQHVGEGDEIIVSNLEHHANIVPWQQLAAAKGAKLRVI 69 E+++ G TE+ NL K ++ + G G+ +I EH A + L A G ++ + Sbjct: 78 EIVWTSGATESNNLALKGAAYALRAQGRGNHLITVPTEHKAVLDTMHTLEKA-GFEVTYL 136 Query: 70 PVDDSGQILLDEYRKLLNDRTRIVAITQVSNALGTVTPVKEIVELAHRAGAKALVDGAQS 129 G I + R L T + ++ V+N +G + P+ E+ E+ VD Q+ Sbjct: 137 QPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQPIAELGEICAAHDVVFHVDAVQA 196 Query: 130 VSHMRVDVQELGADFFVFSGHKVFGPTGIGVVWGKRDV-LEDMPPWQGGGNMIADVTFEK 188 + +D+ L D FS HKV+GP GIG ++ + + L+ GGG+ Sbjct: 197 AGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEAQIDGGGHE-------- 248 Query: 189 TVFQPIPNKFEAGTGNIADAVGLGAAIDYVNKIGIENIARHEHDLLVYGMQQLREIKGVR 248 N +GT VG+G A ++ +E +R L L ++ GV+ Sbjct: 249 -------NGMRSGTLATHQIVGMGEAFALAGRL-LEEESRRIGALRDRLWAGLADLPGVQ 300 Query: 249 LIGTADDKAS---VMSFVLAGYSTEEVGHALNEEGIAVRTGHHC----AQP--ILRRFG- 298 L G A + +SF + T +G L GIAV G C A P +L G Sbjct: 301 LNGCARQRVPHNLNVSFPPTRHGT--LGSQL--LGIAVSAGSACNSAAAAPSFVLLAIGR 356 Query: 299 ----VETTVRPSLAFYNTFDEIDRLATVVRRL 326 VR SL T EID + VR++ Sbjct: 357 SPDVARNAVRFSLGRGTTEAEIDYVVATVRQV 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,070,403 Number of extensions: 47045 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 5 Length of query: 333 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 247 Effective length of database: 1,212,660 Effective search space: 299527020 Effective search space used: 299527020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)