BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0077.p (567 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 552 e-158 gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 381 e-107 gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 62 1e-10 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 552 bits (1422), Expect = e-158, Method: Compositional matrix adjust. Identities = 282/525 (53%), Positives = 356/525 (67%), Gaps = 16/525 (3%) Query: 53 PDVRLPAPKEVDVSTIFPVQESPCFPISRISLIGEAAERFQFALSSVTGQGGALGSCLGT 112 PDVRLPA + P QESPCF + R+ L G + RF + L G G CLG Sbjct: 66 PDVRLPAQPGAAAEAV-PAQESPCFAVHRVVLDGPGSARFDWLLDLPEANPG--GRCLGV 122 Query: 113 EGVNIVIAHMQNALILRGYTTSQIRAATQDLKTGELVLTILPGHVRHIRFAPDASPRGTA 172 +G+N V++ +QNA++ RGY T++I A QD+ +GEL LTI+PG +R IRFA +A PR T Sbjct: 123 QGINAVMSALQNAIVARGYVTTRILAQPQDIASGELRLTIVPGKIRAIRFADNADPRATQ 182 Query: 173 WNALPINAGDILNLRDIEQGLENFKRVSTAQADIEVAPTEAAER-PGDSDLVIRYQQSFP 231 WNALP + GD+LNLRDIEQGLEN KRV TA+AD+++AP+E + PGDSDLVI YQQ P Sbjct: 183 WNALPASPGDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVISYQQGIP 242 Query: 232 FRFNVALDDGGSKATGKYQAGATLSYDNWWTLNDLFYASWNRSLG--HYGDHGVNSYTVH 289 R +++DD G+KATGKYQ AT+S DNW TLNDLFY S++ LG G G S VH Sbjct: 243 IRLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARGSQSQVVH 302 Query: 290 YSVPAGYWMLGGTLSGSRYHQQVAGANNSIIYSGQSENAELKLSRLIYRDAVRKTTLSLK 349 YS+P GYW L T S +RYHQ VAG N + +YSGQSE ELKLSRL+YRDAVRKTTLS K Sbjct: 303 YSLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVRKTTLSFK 362 Query: 350 GLLRNSRNFIDDTEVGPQRHRTSAWEAGVNHREFIGDATVDANLVYRR---ALDKQGREP 406 G +R+S N++DDTEV QR R + WE G+ HREFIG+ T+D L Y+R A D Sbjct: 363 GFVRSSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGEGTLDLALAYKRGTGAFDAMAAPE 422 Query: 407 ESTADLPQLATRYGLFVADATINAPFQI----AGQRLRYNGSVRTQWNRGALPSQDQFAI 462 E+ + +R+ L AD +NAPF + Q +RY R QWNR L QD+F++ Sbjct: 423 EAFG---EGTSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARAQWNRTPLTPQDKFSL 479 Query: 463 GGRYTVRGFDGELILQAERGWLVRNELALALGQGNQELYAGIDAGQVSGPSAQYLLGDRL 522 GGRY+VRGFDGE L AERGW VRNELA ALG QE+YAG+D G+V GPS + L+G RL Sbjct: 480 GGRYSVRGFDGESNLLAERGWYVRNELAAALGASKQEIYAGLDYGEVGGPSTELLVGRRL 539 Query: 523 AGAVIGLRGAFWTVSYDAFIARPLNKPDGFKTADVTGGFSVNLAF 567 AGAV+GLRGA +Y+ F P+++PD FKT+ +T G S+ +F Sbjct: 540 AGAVLGLRGALGRTNYEVFAGVPISQPDRFKTSPLTAGMSLMWSF 584 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 381 bits (979), Expect = e-107, Method: Compositional matrix adjust. Identities = 218/537 (40%), Positives = 313/537 (58%), Gaps = 29/537 (5%) Query: 51 QSPDVRLPAPK-EVDVSTIFPVQESPCFPISRISL-----IGEAAER----------FQF 94 Q P V LP + + D+ + ES CFP+ R++L + EA R F F Sbjct: 54 QQPSVSLPRQRSDEDLQSYTLAPESLCFPVQRVTLAFPEQVSEAVRRTGGFALQMDPFHF 113 Query: 95 ALSSVTGQGGALGSCLGTEGVNIVIAHMQNALILRGYTTSQIRAATQDLKTGELVLTILP 154 + + G G SCLG +G+ ++ + ++ RGY+T+++ QDL TG+L +T++P Sbjct: 114 IQTYLAGYAG---SCLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLVP 170 Query: 155 GHVRHIRFAPDASPRGTAWNALPINAGDILNLRDIEQGLENFKRVSTAQADIEVAPTEAA 214 G + IRF D + + +A P AG +LNLRD+EQGLE KRV++ D+++ PT+ Sbjct: 171 GIIHAIRF-KDPAITASYRSAFPTQAGALLNLRDLEQGLEQMKRVASQDVDMQILPTDV- 228 Query: 215 ERPGDSDLVIRYQQSFPFRFNVALDDGGSKATGKYQAGATLSYDNWWTLNDLFYASWNRS 274 PG SD+V+ ++ P++ ++LDD G+K+TGKYQAG L+ D+ +NDLF A ++ Sbjct: 229 --PGQSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLLGINDLFNAGFSSD 286 Query: 275 LGHYGD-HGVNSYTVHYSVPAGYWMLGGTLSGSRYHQQVAGANNSIIYSGQSENAELKLS 333 G G YS PAGYW G + S SRYHQ++AG + + SG SEN E+++ Sbjct: 287 ADRMGQARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRIAGLFQTFVSSGHSENLEVRVQ 346 Query: 334 RLIYRDAVRKTTLSLKGLLRNSRNFIDDTEVGPQRHRTSAWEAGVNHREFIGDATVDANL 393 +L RDA +K +L L+ R SR++IDDTE+ QR T+ E G HR + GD +DA L Sbjct: 347 KLFQRDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFAELGWMHRHYFGDVQLDATL 406 Query: 394 VYRRAL---DKQGREPESTADLPQLATRYGLFVADATINAPFQIAGQRLRYNGSVRTQWN 450 R + + P+ +D P RY + DAT+ APF++ GQ L Y G++R Q + Sbjct: 407 ANRWGVSWFNGAADAPDRLSDGP--TYRYSMQTIDATVVAPFKLGGQSLTYVGTLRGQHS 464 Query: 451 RGALPSQDQFAIGGRYTVRGFDGELILQAERGWLVRNELALALGQGNQELYAGIDAGQVS 510 R L DQFAIG RYTVRGFDGEL L +E G +RN+L +ALG LYAG+DAG++ Sbjct: 465 RSRLYLTDQFAIGSRYTVRGFDGELTLASESGVFLRNDLEIALGSTGAALYAGLDAGRLY 524 Query: 511 GPSAQYLLGDRLAGAVIGLRGAFWTVSYDAFIARPLNKPDGFKTADVTGGFSVNLAF 567 GPS L GD LAGA IGLRGA + Y+ F L +P+ F+TA GF++ F Sbjct: 525 GPSVANLQGDTLAGAAIGLRGAVSGLHYELFAGWALVRPEQFQTAMPAAGFNLIYQF 581 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 62.0 bits (149), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 111/478 (23%), Positives = 193/478 (40%), Gaps = 73/478 (15%) Query: 77 FPISRISLIGEA---AERFQFALSSVTGQGGALGSCLGTEGVNIVIAHMQNALILRGYTT 133 F I R + G ++ Q A++ +GQG G + + ++N GYT Sbjct: 53 FEIRRFVVAGNTLLPQDQVQAAVAPFSGQGRVYGD------IQQALEALENLYRSAGYTA 106 Query: 134 SQIRAATQDLKTGELVLTILPGHVRHIRFAPDAS-PRGTAWNALP-INAGDILNLRDIEQ 191 Q+ Q+L +GE+ + ++ V ++ + + + ++P + G NLR I + Sbjct: 107 VQVHVPEQELSSGEVRIDVVETVVGKVQVSENRHFSQANIRASIPALKEGVPPNLRRISE 166 Query: 192 GLENFKRVSTAQADIEVAPTEAAERPGDS-DLVIRYQQSFPFRFNVALDDGGSKATGKYQ 250 ++ Q ++ T +A + GD D I + P R + LD+ GS TG+++ Sbjct: 167 SVQLANDNPAKQVNV----TLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSADTGRWR 222 Query: 251 AGATLSYDNWWTLNDLFYASWNRSLGHYGDHGVNSYTVHYSVPAGYWMLGGTL-----SG 305 G + + N + + + ++ S V+ Y++ Y +P + LG +L Sbjct: 223 TGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKVDLYSLGYRLP--LYELGDSLDFIYGKS 280 Query: 306 SRYHQQVAGANNSIIYSGQSENAELKLSRLIYRDAVRKTTLSL--------------KGL 351 S Q N+++ +G+ E L+ + R+ + L L GL Sbjct: 281 SVSSGQTLALNSTLNITGRGEVYALRWNHFFARNGEWSSKLVLGADYKKVDSSCAINGGL 340 Query: 352 LRNSR-NFIDDTEVGPQRHRTSAWEAGVNHREFIGDATVDANL-VYRRALDKQGREPEST 409 L + N P SA GV +DAN+ V R Q R Sbjct: 341 LNGATLNTCLPYSTLPLSLTYSAQRQGVGE-------VIDANIGVARNMATGQARRN--- 390 Query: 410 ADLPQLATRYGLFV-------------ADATINAPFQIAGQRLRYNGSVRTQWNRGALPS 456 D Q+ RY LF A+ FQ Q +R G++ Q + ALP+ Sbjct: 391 -DALQVTDRYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQ-MRLAGNL--QLSPHALPA 446 Query: 457 QDQFAIGGRYTVRGFDGELILQAERGWLVRNE------LALALGQGNQELYAGIDAGQ 508 +QF + G VRGF E + A+ G ++ +E LA + +G+ L A +DAG+ Sbjct: 447 VEQFGLAGATAVRGFT-ERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDAGR 503 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,795,671 Number of extensions: 80663 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 3 Length of query: 567 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 477 Effective length of database: 1,193,720 Effective search space: 569404440 Effective search space used: 569404440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)