BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0059.p (255 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 303 1e-83 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 128 5e-31 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 115 5e-27 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 108 4e-25 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 108 6e-25 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 303 bits (775), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 154/253 (60%), Positives = 182/253 (71%) Query: 3 TTATKITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQALGRKAVALQ 62 T + +I L+TG SRGLG+NTAL +AR G V++TY A+A VAE+QALG A AL+ Sbjct: 5 TQSPRIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAAALR 64 Query: 63 LDTGDVAAFAPFAERLCAALRDTWSRNSIDHLVNNAGHGDYALIGDTTEAQFDGLVNVHF 122 LD D A+F FA+ + AL+ W +LVNN G G +A I +T+EA FD L VH Sbjct: 65 LDLADSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDALFKVHL 124 Query: 123 KGVYFLTQTLLPLIADGGRIVNLSSGLTRTSFPGYAVYAAAKGAVEVLTRYMAKELGARG 182 KG +FLTQ LLPL+A GGRI+N+SSGL R S PGYA YA+ KG VEV+TRYMAKE GARG Sbjct: 125 KGPFFLTQALLPLLAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFGARG 184 Query: 183 IAVNTVAPGAIETDFGGGVVRDNPEINKQFAGLTALGRVGVPDDIGPMIASLLSQDNRWV 242 I VNTVAPGAIETDFGGG VRDN E+N A TALGR G+PDDIG IASLL DN W+ Sbjct: 185 IVVNTVAPGAIETDFGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIASLLRPDNGWI 244 Query: 243 TAQRIEVSGGQGI 255 AQR+EVSGG I Sbjct: 245 NAQRVEVSGGMFI 257 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 128 bits (321), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 89/255 (34%), Positives = 135/255 (52%), Gaps = 17/255 (6%) Query: 2 TTTATKITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQALGRKAVAL 61 +T +K+ V G SRG+G +A G V TY S D+AQA+V+ +QA G +A+A+ Sbjct: 13 STLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAI 72 Query: 62 QLDTGDVAAFAPFAERLCAALRDTWSRNSIDHLVNNAGHGDYALIGDTTEAQFDGLVNVH 121 Q D+ D AA A RL A +T+ R +D LVNNAG + D + V+ Sbjct: 73 QADSADAAALQQ-AIRLAA---ETFGR--LDILVNNAGVLAMGPLDQFKLEDLDRTLAVN 126 Query: 122 FKGVYFLTQTLLPLIADGGRIVNL-SSGLTRTSFPGYAVYAAAKGAVEVLTRYMAKELGA 180 + V+ TQ + +GGR++++ S+ R F G AVYA +K A+ LT+ +A++LG Sbjct: 127 VRSVFVATQEAARYMGEGGRVISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGP 186 Query: 181 RGIAVNTVAPGAIETDFGGGVVRDNP---EINKQFAGLTALGRVGVPDDIGPMIASLLSQ 237 RGI VN V PG ++TD NP + GL AL R G ++I +A L Sbjct: 187 RGITVNNVQPGPVDTDM-------NPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGP 239 Query: 238 DNRWVTAQRIEVSGG 252 ++ ++T + + GG Sbjct: 240 ESGYITGANLMIDGG 254 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 115 bits (287), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 98/258 (37%), Positives = 128/258 (49%), Gaps = 21/258 (8%) Query: 1 MTTTATKITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQALGRKAVA 60 MTTT VTG+SRG+GR AL +AR G D+V+ H+R D A V AEI ALGR+ Sbjct: 1 MTTTGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRV 60 Query: 61 LQLDTGDVAAFAPFAERLCAALRDTWSRNSIDHLVNNAG-HGDYALIGDTTEAQFDGLVN 119 LQ DVA A+ L L D +V NAG D A E +D ++ Sbjct: 61 LQF---DVANREQAAQVL---LADVEQHGCYYGVVCNAGITRDNAFPAMPGE-DWDSVLR 113 Query: 120 VHFKGVY-FLTQTLLPLIAD--GGRIVNLSSGLTRTSFPGYAVYAAAKGAVEVLTRYMAK 176 + G Y L ++P++ GRIV L+S G Y+AAK V T+ +A Sbjct: 114 TNLDGFYNVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALAL 173 Query: 177 ELGARGIAVNTVAPGAIETDFGGGVVRDNP--EINKQFAGLTALGRVGVPDDIGPMIASL 234 EL R I VN VAPG I+TD D P EI K L R G P+++ +A L Sbjct: 174 ELAKRDITVNCVAPGLIDTDM----TEDAPIDEILK----LIPARRAGKPEEVAAAVAFL 225 Query: 235 LSQDNRWVTAQRIEVSGG 252 +S D ++T Q I V+GG Sbjct: 226 MSPDAAYITRQVISVNGG 243 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 108 bits (270), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 86/248 (34%), Positives = 121/248 (48%), Gaps = 12/248 (4%) Query: 7 KITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQALGRKAVALQLDTG 66 K+ +VTG S G+G TA A G V V+ R+D+ + V EI G AV + G Sbjct: 10 KVVVVTGGSEGIGLATAKLFAAEGARVYVS-GRRADKLEQAVREI---GNGAVGV---PG 62 Query: 67 DVAAFAPFAERLCAALRDTWSRNSIDHLVNNAGHG--DYALIGDTTEAQFDGLVNVHFKG 124 D A +RL A ++ R +D L NAG + +G E FDGL N++ +G Sbjct: 63 DAAKLDDL-DRLYARVQADHGR--LDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRG 119 Query: 125 VYFLTQTLLPLIADGGRIVNLSSGLTRTSFPGYAVYAAAKGAVEVLTRYMAKELGARGIA 184 + F Q LPL++ GG IV S FPG ++Y A+K AV R +L RGI Sbjct: 120 LLFSVQKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIR 179 Query: 185 VNTVAPGAIETDFGGGVVRDNPEINKQFAGLTALGRVGVPDDIGPMIASLLSQDNRWVTA 244 VN VAPG ET + P + + LGR+G PD+IG + L S ++ ++ Sbjct: 180 VNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAG 239 Query: 245 QRIEVSGG 252 + V GG Sbjct: 240 VELFVDGG 247 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 108 bits (269), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 95/258 (36%), Positives = 137/258 (53%), Gaps = 13/258 (5%) Query: 1 MTTTATKITLVTGASRGLGRNTALSIARHGGDVVVTYHSRSDEAQAVVAEIQALGRKAVA 60 M A K+ ++GAS G+G A + A G +VV Y+S EA+AV AE++A G K++ Sbjct: 1 MLDLAGKVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSIT 60 Query: 61 LQLDTGDVAAFAPFAERLCAALRDTWSRNSIDHLVNNAGH-GDYALIGDTTEAQFDGLVN 119 + D D +A + A + ID L+NNAG A + T+ FD ++N Sbjct: 61 IGADVRDTSAINAAVAKAVAQM------GRIDVLINNAGALVKRAPLETVTDELFDDIIN 114 Query: 120 VHFKGVYFLTQTLLPLI--ADGGRIVNLSSGLTRT-SFPGYAVYAAAKGAVEVLTRYMAK 176 ++ + V T+ ++P++ GG I+N++S R PG +YAA+KG V LTR MAK Sbjct: 115 INARSVVAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAK 174 Query: 177 ELGARGIAVNTVAPGAIETDFGGGVVRDNPEINKQFAGLTALGRVGVPDDIGPMIASLLS 236 EL A I VN VAPG I T F PE + F +GR+G PD+ L S Sbjct: 175 ELLADKIRVNAVAPGVIMTPFQERFT--TPEQLEGFRKTIPMGRIGEPDECSGAFLYLAS 232 Query: 237 -QDNRWVTAQRIEVSGGQ 253 Q + +VT Q I+V+GGQ Sbjct: 233 EQMSGYVTGQIIDVNGGQ 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 794,601 Number of extensions: 33186 Number of successful extensions: 250 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 45 Length of query: 255 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 172 Effective length of database: 1,226,865 Effective search space: 211020780 Effective search space used: 211020780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)