BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0053.p (352 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 554 e-159 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 373 e-104 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 108 9e-25 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 554 bits (1428), Expect = e-159, Method: Compositional matrix adjust. Identities = 293/378 (77%), Positives = 307/378 (81%), Gaps = 39/378 (10%) Query: 1 MNVFWFIPTHGDSRYLGTAEGGRLVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVTA 60 MNVFWFIPTHGDSRYLGT+EG R VSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWV A Sbjct: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 Query: 61 ASLIGETRQLKFLVAVRPGLSAPTLSARMAATFDRLSGGRLLVNVVTGGDPAEQESDGIF 120 ASLI TR+LKFLVAVRPGL APTL+ARMAATFDRLSGGRLL+NVVTGGDP EQESDG+F Sbjct: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 Query: 121 GTHKERYEISDEFLKIWRDVLSKTHTGGETNFEGKHLSVKGAKALYPPVQAPYPPLYFGG 180 G+H ERYEISDEFLKIWRDVL+KT +G T+F+GKHLSVKGAKALYPPVQ PYPPLYFGG Sbjct: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPEAVAEKIAIVRARAAKAGRTLRFGIRLHVIVRETTEA 240 SSDEAIDLAAEQVDVYLTWGEPP AVAEKIA VR RAAKAGRTLRFGIRLHVIVRET EA Sbjct: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 Query: 241 AWRAADELISKLDDATIEKAQKTFAKMDSEGQRRMAALQF-------------------- 280 AWRAADELIS+LDD TI KAQ FA+MDSEGQRRMAAL Sbjct: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 Query: 281 -------------------XYADLGIDTFILSGYPHLEESYRFAELVFPLLPRKQKETLP 321 YA LGIDTFILSGYPHLEESYRFAELVFPLLPR Q+E Sbjct: 301 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLLPRGQREGGS 360 Query: 322 GFNLTGPFGEIVGNTHVP 339 G L GPFGEIVGNT +P Sbjct: 361 GQQLPGPFGEIVGNTELP 378 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 373 bits (957), Expect = e-104, Method: Compositional matrix adjust. Identities = 191/355 (53%), Positives = 237/355 (66%), Gaps = 45/355 (12%) Query: 1 MNVFWFIPTHGDSRYLGTAEGGRLVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVTA 60 + +FWF+PT GD+RYLG ++ GR + +YM+Q+AV ++TLGYDG+L+PTG SC D WV A Sbjct: 3 LELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWVVA 62 Query: 61 ASLIGETRQLKFLVAVRPGLSAPTLSARMAATFDRLSGGRLLVNVVTGGDPAEQESDGIF 120 ASL+ T+++K LVA+R L P SAR AAT D+ GRLL+NVV GGD E E+DG+F Sbjct: 63 ASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGVF 122 Query: 121 GTHKERYEISDEFLKIWRDVLSKTHTGGET-NFEGKHLSVKGAKALYPPVQAPYPPLYFG 179 H ERYE +DE+L IWR +L GET N+EGKH ++ + +P VQ PYPPLYFG Sbjct: 123 LKHDERYEAADEYLTIWRKLLQ-----GETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFG 177 Query: 180 GSSDEAIDLAAEQVDVYLTWGEPPEAVAEKIAIVRARAAKAGRTLRFGIRLHVIVRETTE 239 GSS A DLAA+ VD YLTWGEPP AV +KI VR RAA+ GRT+RFG+RLH IVRETT Sbjct: 178 GSSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTA 237 Query: 240 AAWRAADELISKLDDATIEKAQKTFAKMDSEGQRRMAALQ-------------------- 279 AW AA+ LISKL D I +AQ+ +A+MDS GQ+RMAAL Sbjct: 238 EAWTAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLV 297 Query: 280 -------------------FXYADLGIDTFILSGYPHLEESYRFAELVFPLLPRK 315 YADLG+DTF+LSGYPHLEE+ RFAELVFPLL + Sbjct: 298 RGGAGTSLVGDATTVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKN 352 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 108 bits (269), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 99/331 (29%), Positives = 153/331 (46%), Gaps = 55/331 (16%) Query: 26 SHDYMKQVAVAADTLGYDGVL----LPTGRSCE---DAWVTAASLIGETRQLKFLVAVRP 78 S +Y +++AV A+ G++ L G E ++ + +L+ T +LK L A+ P Sbjct: 42 SLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 101 Query: 79 GLSAPTLSARMAATFDRLSGGRLLVNVVTGGDPAEQESDGI-FGTHKERYEISDEFLKIW 137 G P + A+ AT D +S GR+ +NVV+G E ++ G + H ERY S+EF++ Sbjct: 102 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 161 Query: 138 RDVLSK---THTGGETNFEGKHLSVKGAKALYPPVQAPYPPLYFGGSSDEAIDLAAEQVD 194 + + ++ T G F LS K PVQ P+P ++ GGSS A D AA D Sbjct: 162 KGIWTQDDFTFKGDFYRFNNYTLSPK-------PVQKPHPEIFQGGSSRAARDNAASVSD 214 Query: 195 VYLTWGEPPEAVAEKIAIVRARAAKAGRTLRFGIRLHVIVRETTEAAWRAADELISKLDD 254 Y T G E + +I ++A+AA+ G +R G+ VI R+T E A ++I D Sbjct: 215 WYFTNGNTLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDII---DH 271 Query: 255 ATIE------KAQKTFAKMDSEGQRRMAALQF----XYAD-------------------- 284 A +E A K K EG+ A F Y D Sbjct: 272 AHVEAVHAFGDAVKQAGKASPEGEGNWAKSTFEDLVQYNDGFKTNLIGTPQQIAERIVAL 331 Query: 285 --LGIDTFILSGYPH-LEESYRFAELVFPLL 312 +G+D +L+G+ H +EE F + V PL+ Sbjct: 332 KAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,129,097 Number of extensions: 46235 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 5 Length of query: 352 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 266 Effective length of database: 1,212,660 Effective search space: 322567560 Effective search space used: 322567560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)