BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0052.p (322 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 513 e-147 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 291 4e-80 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 282 3e-77 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 247 9e-67 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 135 3e-33 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 513 bits (1321), Expect = e-147, Method: Compositional matrix adjust. Identities = 255/328 (77%), Positives = 286/328 (87%), Gaps = 7/328 (2%) Query: 1 MSAQQ----RSARRRTLGLLTTLAIAA--AGLGVSNFAAAQSRQVLRIGYQKAASTLVLL 54 MSA + ARRR + L+ +A+ A LG S A Q ++LRIGYQKAA+TLVLL Sbjct: 1 MSANKNKTVHQARRRAIQRLSAIALGALTIALGASPVQA-QETKLLRIGYQKAANTLVLL 59 Query: 55 KAHGSLEKRLQAQGVEVKWAEFTAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGANFVYT 114 KAHG+LEKRLQ GVEVKWAEF AGPQLLEALNVGSVDFGYVGEAPPVFAQAAGA+FVYT Sbjct: 60 KAHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYT 119 Query: 115 AYEIPTPDAEGLLVQKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLSYSDIQPV 174 AYEIPTPDAEGLLVQK+SPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGL YSDIQPV Sbjct: 120 AYEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPV 179 Query: 175 YLAPADARAAFEKGAIDAWAIWDPFQAAAEKQIGARRLATGVGAVSHHQFFLSARPFAEK 234 YLAPADARAAFE+GA+DAWAIWDPFQAAAEKQ+GARRLATGVGAV+HHQFFLSARPFA K Sbjct: 180 YLAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAK 239 Query: 235 NAAIIKVLLEEITKEGQWIRANTKEAATQLAPIQGLDTDVIEISLKRYEHIFRPIDAKVL 294 N + K+LLEEI++EG+W+R +T +AA QLAPIQGLD D+IE LKRY H+++P+DA+VL Sbjct: 240 NPQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVL 299 Query: 295 EEQQRIADAFFELKLIPKKLSVKDAVLK 322 EQQ+IADAF+ELKLIPK L V DAVLK Sbjct: 300 AEQQKIADAFYELKLIPKPLKVSDAVLK 327 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 291 bits (746), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 147/287 (51%), Positives = 202/287 (70%), Gaps = 3/287 (1%) Query: 33 AAQSRQVLRIGYQKAASTLVLLKAHGSLEKRLQAQGVEVKWAEFTAGPQLLEALNVGSVD 92 AA RQ LR+GYQK A L L++AHG LE+RL G VKW EF+AGP LEAL GS+D Sbjct: 47 AAAPRQ-LRVGYQKGA--LSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSID 103 Query: 93 FGYVGEAPPVFAQAAGANFVYTAYEIPTPDAEGLLVQKDSPIKSVAELKGKKIAFNKGSD 152 FG VGEAPP+FAQAAG VY +P P E +LV+ DS ++ +LKGK+IA NKGS+ Sbjct: 104 FGDVGEAPPIFAQAAGTPLVYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSN 163 Query: 153 VHWLVVALLKKAGLSYSDIQPVYLAPADARAAFEKGAIDAWAIWDPFQAAAEKQIGARRL 212 VH+ +V LL+K GL Y D+ PV+LAPADARAAFE+GA+DAWAIW+P+ AAA++ + AR L Sbjct: 164 VHYFLVKLLEKHGLKYGDVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARIL 223 Query: 213 ATGVGAVSHHQFFLSARPFAEKNAAIIKVLLEEITKEGQWIRANTKEAATQLAPIQGLDT 272 A G V + ++ S R +A +A ++++ LEE+ K W +A E A++LA I GL Sbjct: 224 ADADGVVKNRAYYFSTRDYARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPR 283 Query: 273 DVIEISLKRYEHIFRPIDAKVLEEQQRIADAFFELKLIPKKLSVKDA 319 V+++ ++RY + PI +L+EQQ+IAD F +L+LIP+K+S+ +A Sbjct: 284 SVLDVVVRRYHYGTLPITRAILDEQQQIADTFLDLRLIPRKISLLEA 330 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 282 bits (721), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 141/316 (44%), Positives = 205/316 (64%), Gaps = 3/316 (0%) Query: 7 SARRRTLGLLTTLAIAAAGLGVSNFAAAQSRQVLRIGYQKAASTLVLLKAHGSLEKRLQA 66 S RRR L T L + +A S FA Q + +RIGYQK+++ L +LKA+G+LEK L Sbjct: 8 STRRRLLHAATALGLGSALAPASVFA--QGNKAIRIGYQKSSTLLTILKANGTLEKLLAP 65 Query: 67 QGVEVKWAEFTAGPQLLEALNVGSVDFGY-VGEAPPVFAQAAGANFVYTAYEIPTPDAEG 125 G ++ W EF +G LLEALNVG VD V + PVFAQAAGA Y A E P+P A+ Sbjct: 66 TGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSPAAQA 125 Query: 126 LLVQKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLSYSDIQPVYLAPADARAAF 185 ++V+ DS ++ ++LKGKKIA K + VH+L++A L+KAGL +SD+ YL+PAD RAAF Sbjct: 126 IVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGRAAF 185 Query: 186 EKGAIDAWAIWDPFQAAAEKQIGARRLATGVGAVSHHQFFLSARPFAEKNAAIIKVLLEE 245 E+G++DAW WDPF A ++Q R L+ G G + +++L++ FA+ +++V+ +E Sbjct: 186 ERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVVFDE 245 Query: 246 ITKEGQWIRANTKEAATQLAPIQGLDTDVIEISLKRYEHIFRPIDAKVLEEQQRIADAFF 305 + K G W++ + KEAA LAP+ GLD D IE++ R + R + + L EQQRIAD FF Sbjct: 246 LQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQQRIADTFF 305 Query: 306 ELKLIPKKLSVKDAVL 321 L+P+K++ D + Sbjct: 306 AAGLLPRKINTNDVAI 321 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 247 bits (631), Expect = 9e-67, Method: Compositional matrix adjust. Identities = 139/321 (43%), Positives = 194/321 (60%), Gaps = 5/321 (1%) Query: 1 MSAQQRSARRRTLGLLTTLAIAAAGLGVSNFAAAQSRQVLRIGYQKAASTLVLLKAHGSL 60 M A Q +RR L L LA A G AA + VLRIGYQK +TL +LK G L Sbjct: 1 MDAHQPRHKRRLL--LQGLAAGALASGTPLVRAAADK-VLRIGYQKF-NTLNILKGSGKL 56 Query: 61 EKRLQAQGVEVKWAEFTAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGANFVYTAYEIPT 120 E+ LQ QG VKW EF AGPQL EALN ++D G+ + P VFA A+G N VY E P Sbjct: 57 EQALQPQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPY 116 Query: 121 PDAEGLLVQKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLSYSDIQPVYLA-PA 179 P G+ V ++SPI+SVA+LKGKK+A +G +V +L++ L++ GLSY DI+PVY+ A Sbjct: 117 PKGIGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAA 176 Query: 180 DARAAFEKGAIDAWAIWDPFQAAAEKQIGARRLATGVGAVSHHQFFLSARPFAEKNAAII 239 D RAAF+ G +DA +WDPF A E R L G G ++ F L+ F +++ I+ Sbjct: 177 DVRAAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIV 236 Query: 240 KVLLEEITKEGQWIRANTKEAATQLAPIQGLDTDVIEISLKRYEHIFRPIDAKVLEEQQR 299 + + E+ K QW +++ +E A LAP G+D V+ ++ +R + +DA ++ EQQ+ Sbjct: 237 RTVFAELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQ 296 Query: 300 IADAFFELKLIPKKLSVKDAV 320 IADAF +L LI + V+D V Sbjct: 297 IADAFLKLGLIKNPVRVQDKV 317 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 135 bits (341), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 97/272 (35%), Positives = 143/272 (52%), Gaps = 9/272 (3%) Query: 19 LAIAAAGLGVSNF-------AAAQSRQVLRIGYQKAASTLVLLKAHGSLEKRLQAQGVEV 71 L +A A LGV+ +AAQ +V+R+ Y + ++L+ G LE+ L+AQG EV Sbjct: 7 LRLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLKAQGTEV 66 Query: 72 KWAEFTAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGANFVYTAYEIPTPDAEGLLVQKD 131 KW + LE LN SVDFG + A+A G N V Y P+ L V K+ Sbjct: 67 KWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANG-NPVKAVYIYSRPEWTALAVGKE 125 Query: 132 SPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLSYSDIQPVYLAPADARAAFEKGAID 191 SP+KS+ ELKGKKIA KG+D + ++ L + GL SD++ V+L AD RAA E+G + Sbjct: 126 SPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALEQGRVA 185 Query: 192 AWAIWDPFQAAAEKQIGARRLATGVGAVSHHQFFLSARPFAEKNAAIIKVLLEEITKEGQ 251 AWA DP AA+E + G+R + V + + F + FA ++ +K ++ + Sbjct: 186 AWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDSFAARHPDQVKRVIAAYERARS 244 Query: 252 WIRANTKEAATQLAPIQGLDTDVIEISLKRYE 283 WI A+ E L+ L V + LKR + Sbjct: 245 WIIAHPDETVALLSEESKLSAPVARLQLKRND 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 873,169 Number of extensions: 34999 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 148 Number of HSP's successfully gapped: 7 Length of query: 322 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 237 Effective length of database: 1,217,395 Effective search space: 288522615 Effective search space used: 288522615 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)