BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0031.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 580 e-167 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 238 5e-64 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 207 1e-54 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 147 8e-37 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 145 6e-36 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 580 bits (1495), Expect = e-167, Method: Compositional matrix adjust. Identities = 278/323 (86%), Positives = 303/323 (93%) Query: 1 MKPKIFVARAVFPEVLERLAQHFDVESNQEDRIFSADELIAKVQGKAGLFSTPSEPVSAA 60 MKPKIFVARAVFPEVL RL QHF+VESNQ+DRI+SA EL AK QGK GLF+TPSEPV+AA Sbjct: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVTAA 60 Query: 61 LFAANPQLKAVCNMAVGYNNIDIAAATKAGVQATNTPDVLNETTADFGWALLMATARRVT 120 LFAANPQLKAVCNMAVGYNNID+AAAT+AGV ATNTPDVLNETTADFGWAL+MA ARR+T Sbjct: 61 LFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAARRIT 120 Query: 121 ESEHWLRAGHWKKWSYDSFTGPDVHGATLGIIGMGRIGQAIARRSLGFDMEVIYHNRSQL 180 ESEH+LRAG WKKWSYDSF GPD+HGATLGIIGMGRIGQAIARRSLGFDM+V+YHNRS+L Sbjct: 121 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL 180 Query: 181 SPELEARANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINLARG 240 +PELEARAN+A+YV KEELLR ADHVILVLPYSK++HHTI AA+LALMKP ATL N+ARG Sbjct: 181 APELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 Query: 241 GIVDDAALIAALRDRKIAAAGLDVFENEPAFHPDFLGLSNVVLTPHIASASTPTRLAMAN 300 GIVDDAALIAALR+ +IAAAG+DVFENEPAF P+FL LSNVVLTPHIASASTPTRLAMAN Sbjct: 241 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 Query: 301 CAADNLIAALSGQTPPNLLNRDV 323 CAADNLIAALSGQ PPNLLN DV Sbjct: 301 CAADNLIAALSGQRPPNLLNPDV 323 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 238 bits (607), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 143/317 (45%), Positives = 193/317 (60%), Gaps = 9/317 (2%) Query: 1 MKPKIFVARAVFPEVLERLAQHFDVESNQEDRIFSAD--ELIAKVQGKAGLFSTPSEPVS 58 MKP + V + + +++RL FD+ DRI + + IA + G+ S P++ Sbjct: 1 MKPHVVVYKKLPEHLVQRLQAEFDL--TLFDRITDDNRADFIAALATAHGMIGA-SLPIT 57 Query: 59 AALFAANPQLKAVCNMAVGYNNIDIAAATKAGVQATNTPDVLNETTADFGWALLMATARR 118 A+ A PQLK V ++VG +N D+ + G+ +TP VL E TAD +AL++A+ARR Sbjct: 58 NAMLEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARR 117 Query: 119 VTESEHWLRAGHWKKWSYDSFTGPDVHGATLGIIGMGRIGQAIARRS-LGFDMEVIYHNR 177 V E +++AG WK ++ G +VHG TLG+IGMGRIG A+ARR+ GF M ++YHNR Sbjct: 118 VVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNR 177 Query: 178 SQLSPELEARANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINL 237 PE E R A+YV KE+LL AD V ++LP + ++ A + ALMK A IN Sbjct: 178 RP-DPEAE-RELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINA 235 Query: 238 ARGGIVDDAALIAALRDRKIAAAGLDVFENEP-AFHPDFLGLSNVVLTPHIASASTPTRL 296 +RG IVD+AALIAAL+D+ I AGLDVFE EP L L NVV PHI SA+ TRL Sbjct: 236 SRGRIVDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRL 295 Query: 297 AMANCAADNLIAALSGQ 313 AMA A NLIA L G+ Sbjct: 296 AMAELAVTNLIAGLRGE 312 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 207 bits (526), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 125/312 (40%), Positives = 176/312 (56%), Gaps = 11/312 (3%) Query: 2 KPKIFVARAVFPEVLERLAQHFDVESNQEDRIFSADELIAKVQGKAGLFSTPSEPVSAAL 61 +P + + + +L+ L QH+ N + S EL A G+ + VS Sbjct: 6 RPHVLIVARLPQHLLDLLQQHYTCH-NLILQPHSEAELAAIAPQIRGIAANGEAKVSREF 64 Query: 62 FAANPQLKAVCNMAVGYNNIDIAAATKAGVQATNTPDVLNETTADFGWALLMATARRVTE 121 A P L+ V VGY+ +D+ AA + G+ T+TPDVLN+ AD AL++ATAR V Sbjct: 65 MARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATARNVVR 124 Query: 122 SEHWLRAGHWKKWSYDSFTGPDVHGATLGIIGMGRIGQAIARRSLGFDMEVIYHNRSQLS 181 ++ + R+G WKK + T V GA LGI+G+GRIGQAIA+R+ FDM++ YHNRS+ Sbjct: 125 ADRFARSGEWKKGPFPFTT--KVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR-- 180 Query: 182 PELEARANNAKYVGK-EELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINLARG 240 + YV L R D ++++ P + + A L + P+ LIN+ARG Sbjct: 181 -----KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 Query: 241 GIVDDAALIAALRDRKIAAAGLDVFENEPAFHPDFLGLSNVVLTPHIASASTPTRLAMAN 300 +VD+AALIAAL+ IA AGLDVF +EP + L NVVLTPH+AS + TR AMA+ Sbjct: 236 SVVDEAALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMAD 295 Query: 301 CAADNLIAALSG 312 A +NL A SG Sbjct: 296 LAFNNLQAHFSG 307 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 147 bits (372), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 99/294 (33%), Positives = 147/294 (50%), Gaps = 15/294 (5%) Query: 1 MKPKIFVARAVFPEVLERLAQHFDV--ESNQEDRIFSADELIAKVQGK-AGLFSTPSEPV 57 MKP + V + E +A H+D+ +++ R DE+IA K A + + S + Sbjct: 1 MKPPLLVLIHLSEESTATIAAHYDILYAPDRQRR----DEMIAGPARKVAVVLTNGSTGL 56 Query: 58 SAALFAANPQLKAVCNMAVGYNNIDIAAATKAGVQATNTPDVLNETTADFGWALLMATAR 117 +A A P L+ VC + G+ N+D+A A G++ + AD LL+A R Sbjct: 57 TAEEMRALPHLQLVCTLGAGFENVDVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILR 116 Query: 118 RVTESEHWLRAGHWKKWSYDSFTGPDVHGATLGIIGMGRIGQAIARRSLGFDMEVIYHNR 177 + + + R G W++ P + G +GI+GMG IG+ IARR+ FD E+ Y NR Sbjct: 117 NIPVLDRYTRDGGWRE---TIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNR 173 Query: 178 SQLSPELEARANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINL 237 + + + +L AD +I+ P ++ H I A L + PQ L+N+ Sbjct: 174 KKRD-----DVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNI 228 Query: 238 ARGGIVDDAALIAALRDRKIAAAGLDVFENEPAFHPDFLGLSNVVLTPHIASAS 291 RG IVD AL AAL ++A AGLDV+E EP + L NVVLTPHIA S Sbjct: 229 GRGSIVDTDALGAALSSGRLAGAGLDVYEGEPQPPAALIALPNVVLTPHIAGWS 282 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 145 bits (365), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 93/257 (36%), Positives = 135/257 (52%), Gaps = 11/257 (4%) Query: 57 VSAALFAANPQLKAVCNMAVGYNNIDIAAATKAGVQATNTPDVLNETTADFGWALLMATA 116 VSA + A P L+ + G ++ID AA + G+ A+ WAL+MA A Sbjct: 60 VSARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACA 119 Query: 117 RRVTESEHWLRAGHWKKWSYDSFTGPDVHGATLGIIGMGRIGQAIARRSLGFDMEVIYHN 176 + V + +R GHW K ++ S ++ G TLG++G+G IG+ +A + M V+ H+ Sbjct: 120 KNVVGLDQRMREGHWDKSTHKSL---ELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAHD 176 Query: 177 RSQLSPELEARANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLIN 236 P + + V + L +D V L P + E+ H I A LA MK A L+N Sbjct: 177 -----PYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVN 231 Query: 237 LARGGIVDDAALIAALRDRKIAAAGLDVFENEP--AFHPDFLGLSNVVLTPHIASASTPT 294 ARGG++D+ ALIAAL K+ AAGLD FE EP A HP + N VL+PHI ++ Sbjct: 232 TARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHP-LQRVGNAVLSPHIGGVTSDA 290 Query: 295 RLAMANCAADNLIAALS 311 +AM AA N++A L Sbjct: 291 YIAMGTGAASNVLAVLG 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 977,974 Number of extensions: 38085 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 11 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)