BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0027.p (534 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 490 e-139 gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 365 e-102 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 104 2e-23 gi|300312955|ref|YP_003777047.1| amino acid ABC transporter peri... 101 1e-22 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 74 3e-14 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 490 bits (1262), Expect = e-139, Method: Compositional matrix adjust. Identities = 239/379 (63%), Positives = 301/379 (79%), Gaps = 4/379 (1%) Query: 24 AENIRIGEINSYTGVAQSSQAYRNGADLAVAEINAGGGLLGRNLELISRDDSGSPDEAVR 83 AE IRIGEINS ++ + QAYRNG LA+ +INA GG+ GR L+L+ R+D G D+A R Sbjct: 35 AEAIRIGEINSRHRLSTAGQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDADQAAR 94 Query: 84 HAEALIKTDRVDVLTGSMLSNVALAVAKTAASEKKLFVVGDALSDAITLDKGNRYTFRLR 143 +A ALI D V+VLTGSMLS+VALAV++ AA + + VVGDALSDAITLDKGNRYTFR+R Sbjct: 95 NARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVVGDALSDAITLDKGNRYTFRVR 154 Query: 144 PSTYTQAAMLAEAAAKLPGRRWAIVAPNYEYGQSAVANFKLLLKERRPDIEFVAEQWPAL 203 PSTY QAAMLAE AA+LPGRRWA+VAPNYEYGQSAVA+FKLLLK+ RPD+EFV++QWPAL Sbjct: 155 PSTYMQAAMLAEQAARLPGRRWAVVAPNYEYGQSAVASFKLLLKQARPDVEFVSDQWPAL 214 Query: 204 GKMRAEVVVKALRQARPDAIFNAAFGSDLQQFAEEGSRQGLFAATTVFSM-SAG-PTLGD 261 GK++A+ V +A+RQ+RPD IFNA FG DLQ+F + G+RQ LF V SM SAG P+L Sbjct: 215 GKIKADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRVVSMASAGAPSLDP 274 Query: 262 VLAQYEGTKNWVVLGYPSEQLATPEHQRFREAYLKRFNDRPLLQAVFGYSTVMSIAAGIK 321 +Q E ++W+ +GYP +Q++ PEHQRF EAYLKRF++RP L AVFGY+T+M+IAAG+K Sbjct: 275 --SQSEAIRDWLAIGYPWDQVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAAGLK 332 Query: 322 KAGSVDSEKLVTAMRGLGVETPVGPITFRAHDQQSSMGAFVATLVCKDGRSNMTEWHYLD 381 K+ D E LV AMRGL +P+GP++FRA DQQ+S+GAFV + KDGRS+M +W YLD Sbjct: 333 KSEGGDGEVLVEAMRGLQFGSPLGPVSFRALDQQASLGAFVGLVSQKDGRSSMRDWRYLD 392 Query: 382 GARYMPDDNYIKGRRPASA 400 GA Y+P Y++ RRPASA Sbjct: 393 GAAYLPPPAYVRSRRPASA 411 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 365 bits (937), Expect = e-102, Method: Compositional matrix adjust. Identities = 191/382 (50%), Positives = 255/382 (66%), Gaps = 4/382 (1%) Query: 19 TVAAQAEN-IRIGEINSYTGVAQSSQAYRNGADLAVAEINAGGGLLGRNLELISRDDSGS 77 +VAA+A++ IRIGEINSY + YR G ++A+ EINA GG+LG+ LE+I+RDD+G+ Sbjct: 23 SVAARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDEINASGGVLGKKLEVIARDDNGN 82 Query: 78 PDEAVRHAEALIKTDRVDVLTGSMLSNVALAVAKTAASEKKLFVVGDALSDAITLDKGNR 137 P ++VR AE L+ +RV +L G LSN LA+ A + F+ + L+D I GN+ Sbjct: 83 PGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQHRVFFLAAEPLTDKIVWQNGNK 142 Query: 138 YTFRLRPSTYTQAAMLAEAAAKLPGRRWAIVAPNYEYGQSAVANFKLLLKERRPDIEFVA 197 YT+RLRPST A + + A KL +RWAIV PNYEYGQSAVA+FK L+K+ +PD+EFVA Sbjct: 143 YTYRLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYGQSAVASFKTLMKQAQPDVEFVA 202 Query: 198 EQWPALGKMRAEVVVKALRQARPDAIFNAAFGSDLQQFAEEGSRQGLFAATTVFSMSAG- 256 EQ P LGK+ A V +AL A+PDAIFNA F +DL +F EG+ +GLF V S+ G Sbjct: 203 EQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKFVREGNTRGLFEQVDVVSLLTGE 262 Query: 257 PTLGDVLAQYEGTKNWVVLGYPSEQLATPEHQRFREAYLKRFNDRPLLQAVFGYSTVMSI 316 P D L + E +NW+V GYP + TPEH++F EAY K+FND P ++ GYS +MSI Sbjct: 263 PEYLDPL-KGEAPQNWIVTGYPWYAIHTPEHKKFVEAYRKKFNDYPRNGSLVGYSALMSI 321 Query: 317 AAGIKKAGSVDSEKLVTAMRGLGVETPVGPITFRAHDQQSSMGAFVATLVCKDGRSNMTE 376 AAG++KAGS D+++L A GL VETPV I +R D QS++GAFV K+GR MT Sbjct: 322 AAGLRKAGSTDADRLAAAFSGLKVETPVASIVYRPQDHQSTLGAFVGRTGLKEGRGVMTS 381 Query: 377 WHYLDGARY-MPDDNYIKGRRP 397 + Y+DGA +PD K R P Sbjct: 382 FTYIDGAALQLPDAEVKKLRAP 403 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 113/387 (29%), Positives = 181/387 (46%), Gaps = 25/387 (6%) Query: 1 MKHALTGLAAALALGLAMTVAAQAENIRIGEINSYTG-VAQSSQAYRNGADLAVAEINAG 59 M + AA + LA+T A A+ I+IG YTG A + R+G LAVAEINA Sbjct: 1 MTYKTQAFVAAALMSLALTSQA-ADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAA 59 Query: 60 GGLLGRNLELISRDDSGSPDEAVRHAEALIKTDRVDVLTGSMLSNVALAVAKTAASEKKL 119 GGLLGR ++LI RDD + V+ A+ LI ++V G + + VALA ++ E K+ Sbjct: 60 GGLLGRQIQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALA-SQRFYQEAKI 118 Query: 120 FVV-----GDALSDAITLDKGNRYTFRLRPSTYTQAAMLA-EAAAKLPGRRWAIVAPNYE 173 V+ G ++ +K N Y FR + QA M+A EA K + AI+A + Sbjct: 119 PVINNVATGTVITKQFEKEKDN-YVFRTSAADKIQAKMIADEAVDKQKFTKVAILADSTN 177 Query: 174 YGQSAVANFKLLLKERRPDIEFVAEQWPALGKMRAEVVVKALRQARPDAIFNAAFGSDLQ 233 YGQ + + +L +++ + VA + + + + +Q A+ G +L Sbjct: 178 YGQLGREDLEKVLADKK--VTPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIGPELA 235 Query: 234 QFAEEGSRQGLFAATTVFSMSAGPTLGDVLAQYEGTKNWVVLGYPSEQLA-TPEHQRFRE 292 Q A G + + + S ++G+ + N + Q TP+ + F E Sbjct: 236 QIA-NGMEKLNWHVPIIGSWPL--SMGNFIDNSGKNGNGARMPQTFIQDGNTPKRKAFIE 292 Query: 293 AYLKRFN-DR--PLLQAVFGYSTVMSIAAGIKKAGSVDSEKLVTAMRGLGVETPVGPIT- 348 AY K + DR + A GY +++ +AA IK+AG+ D +K+ A+ L + G +T Sbjct: 293 AYQKAYKVDRMPSAVSAAQGYDSILLLAAAIKQAGTTDGDKVRAALENLNTKVE-GVVTT 351 Query: 349 ----FRAHDQQSSMGAFVATLVCKDGR 371 F D ++ A V K+GR Sbjct: 352 YDKPFTHDDHEAIKPAMVVMGEVKNGR 378 >gi|300312955|ref|YP_003777047.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 392 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 104/357 (29%), Positives = 167/357 (46%), Gaps = 30/357 (8%) Query: 9 AAALALGLAMTVAAQAENI-RIGEINSYTGVAQSSQA-YRNGADLAVAEINAGGGLLGRN 66 A +A+ + ++ AQA ++ ++G + + +G A + R G LA+ + G L G Sbjct: 10 AVMMAMAVGASLPAQAADVVKVGFLTTLSGPASAPGVDEREGFMLALKTL--GNKLGGVP 67 Query: 67 LELISRDDSGSPDEAVRHAEALIKTDRVDVLTGSMLSNVALAVAKTAASEKKLFVVGDAL 126 E+I DD SPD A + A+ LIK ++VD+L+G + SN+ LAVA A + K + V +A Sbjct: 68 AEVIVVDDQFSPDAAKQGADRLIKREKVDILSGFVYSNILLAVAPAAFASKTVVVSANAG 127 Query: 127 SDAITLDKGNRYTFRLRPSTYTQAAMLAEAAAKLPG----RRWAIVAPNYEYGQSAVANF 182 + N Y F S Q EAA KL +R AI+APNY G+ A A F Sbjct: 128 PAQLAGAGCNPYFF----SASYQNDAAHEAAGKLMADKGYQRVAIIAPNYPAGKDASAGF 183 Query: 183 KLLLKERRPDIEFVAEQWPALGKMRAEVVVKALRQARPDAIF---NAAFGSD-LQQFAEE 238 K LK + P +E +P LG++ + LR A DA++ G + ++Q+A Sbjct: 184 KRFLK-KDPAVEL----FPKLGQLDFATEIAQLRAANIDALYFFLPGGMGVNFVKQYAAA 238 Query: 239 GSRQGLFAATTVFSMSAGP-TLGDVLAQYEGTKNWVVLGYPSEQLATPEHQRFREAYLKR 297 G + + + GD++A T W S L +++ F AYLK Sbjct: 239 GMDPKIVRVMPAYDADQQMLSNGDLIAGVANTGQW------SSDLPNEQNRAFVAAYLKE 292 Query: 298 FNDRPLLQAVFGYSTVMSIAAGIKKAGSV--DSEKLVTAMRGLGVETPVGPITFRAH 352 + +P + A Y M I + +K G + E L+ A++ ++ G F A+ Sbjct: 293 YGRQPSMFASQAYDAAMLIDSAVKAVGGKVENREALIAAIKTANFKSVRGGFRFAAN 349 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 97/355 (27%), Positives = 163/355 (45%), Gaps = 26/355 (7%) Query: 11 ALALGLAMTVAAQAENIRIGEINSYTG-VAQSSQAYRNGADLAVAEINAGGGLLGRNLEL 69 AL +G ++ AA A +I++G + TG A+ A +NG LA EINA GG+ G L L Sbjct: 12 ALLIGATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDKLAL 71 Query: 70 ISRDDSGSPDEAVRHAEALIKTDRVDVLTGSMLSNVALAVAKTAASEKKLFVVGDA-LSD 128 + D+ G +EA+ + LI D+V + G LSN A A A A+ K+ V G + +D Sbjct: 72 VIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFA-ADPIANAAKVVVFGTSNTAD 130 Query: 129 AITLDKGNRYTFRLRPSTYTQAAMLAEAAAKLPG-RRWAIVAPNYE-YGQSAVANFKLLL 186 IT +TFR + +AA K G ++ A++ N + + +S FK L Sbjct: 131 GIT--AMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKATL 188 Query: 187 KERRPDIEFVAEQWPALGKMRAEVVVKALRQARPDAIFNAAFGSD----LQQFAEEGSRQ 242 + ++ I + A G + + + ++ PDAI + + + Q G +Q Sbjct: 189 EHQK--IPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMKQ 246 Query: 243 GLFAATTVFSMSAGPTLGDVLAQYEGTKNWVVLGYP-SEQLATPEHQRFREAYLKRFNDR 301 + S P L ++ A+ G + ++G P S + TP ++ F AY +F Sbjct: 247 PFIGGNGLNS----PKLFEI-AKDAG--DNTLMGSPWSAENQTPANKAFITAYKAKFGAD 299 Query: 302 PLLQAVFGYSTVMSIAAGIKK---AGSV--DSEKLVTAMRGLGVETPVGPITFRA 351 P A Y + +A +K +G++ D + L A+ + ++ G FRA Sbjct: 300 PDQFAAQAYDAMYIVADALKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRA 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,481,898 Number of extensions: 59672 Number of successful extensions: 248 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 244 Number of HSP's successfully gapped: 20 Length of query: 534 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 444 Effective length of database: 1,193,720 Effective search space: 530011680 Effective search space used: 530011680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)