BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0010.0028.p
         (141 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspiril...    99   2e-22

>gi|300312905|ref|YP_003776997.1| glutamate synthase
          [Herbaspirillum seropedicae SmR1]
          Length = 531

 Score = 98.6 bits (244), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 43/73 (58%), Positives = 54/73 (73%), Gaps = 3/73 (4%)

Query: 20 HVVSWWWAAAPLALSMIGVRDIVQRRHAVLRNYPLMGHFRFLFEAIRPEIRQYFFEGENX 79
          H + W W   PLAL+ +G+ D++Q+RHAVLRNYP++GHFRF+FEAIRPE+RQYFFE E  
Sbjct: 26 HQLGWGWGLIPLALTAVGLHDLLQKRHAVLRNYPIIGHFRFMFEAIRPELRQYFFEDE-- 83

Query: 80 ISAPRPTSTAARS 92
              RP S   RS
Sbjct: 84 -MDGRPFSRKKRS 95


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.129    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 236,987
Number of extensions: 6420
Number of successful extensions: 30
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 1
Length of query: 141
Length of database: 1,619,870
Length adjustment: 76
Effective length of query: 65
Effective length of database: 1,260,010
Effective search space: 81900650
Effective search space used: 81900650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 126 (53.1 bits)