BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0027.p (711 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 1132 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 509 e-145 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 440 e-124 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 410 e-115 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 382 e-107 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1132 bits (2929), Expect = 0.0, Method: Compositional matrix adjust. Identities = 548/684 (80%), Positives = 601/684 (87%), Gaps = 1/684 (0%) Query: 18 SIARPPRLLETLLANLDGMVYRCRDDEEWTMEFVSEGCLELTGYQPEQLLFNHRISYEDI 77 +I R PRLLETLL NLDG+VYRCRDD +WTMEFVSEGCL LTGY PE LL N R+S+ ++ Sbjct: 2 AIPRSPRLLETLLVNLDGVVYRCRDDAQWTMEFVSEGCLALTGYLPEDLLLNQRLSFLEL 61 Query: 78 THPEDREKVRRTIQSCLDGKRRFELEYRIVRADGEVRWVWERGVGIYNAAGTLDALEGYV 137 THPEDR VR + +C+ +RR ++EYRIVRADG VRWVWERGVGIYNA G ++A+EG++ Sbjct: 62 THPEDRTLVRNHVDACMRERRRIDIEYRIVRADGAVRWVWERGVGIYNANGQVEAIEGFL 121 Query: 138 QDVTARKTADQALLEAERRYRSIFENAIEGVFQSTPDGGYIAVNPALARIYGYKSADDLI 197 QDVT RK A QAL EAERRYRSIFENAIEGVFQ+TPDG YIAVNPALARIYGY S +DLI Sbjct: 122 QDVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLI 181 Query: 198 VSLRDIKHQLYVEPERRTEFMRMMAEFGSVSNFESRVYRRDGEIIWISENARAVRDEAGT 257 V LRDI HQLYVEPERR EFMR+M E GSVSNFESRVYRRDGEIIWISENARAV DEAG Sbjct: 182 VGLRDISHQLYVEPERRQEFMRLMEEQGSVSNFESRVYRRDGEIIWISENARAVYDEAGK 241 Query: 258 LMFYEGTVEAITERKLYEAEIRHQATHDALTGLPNRTMLHNHLQRAIQSTRQKGTLTAVV 317 L YEGTVEAITERK +EAE+RHQATHDALTGLPNR MLH HLQRA++ RQKG LTAV Sbjct: 242 LSCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVA 301 Query: 318 FVDLDQFKFINDSLGHQVGDELLKTVAQRLVSCVRETDMVARQGGDEFVLVLQNQPD-EN 376 FVDLDQFKFINDSLGHQVGDELLKTVAQRL +C+RE DMVARQGGDEFVLVLQNQ + E Sbjct: 302 FVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEA 361 Query: 377 RITEVLQRILTAVARPWVVGDREFHVTCSIGVSRCPTDGRDVETLLKNADSAMYKAKELG 436 I EV+QRIL AVARPW GDREF VT SIGVSR P DG+DVE+LLK+ADSAMY+AKE G Sbjct: 362 GIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQG 421 Query: 437 RNNFQYFAGWMDAQVSNRLEMLVSLRRALDRDELLLYYQPKISLASGRIIGAEALIRWIT 496 RNNFQ+FA WMD+QVSNRLEML++LRRALD+DE LYYQPK+SL GR+IGAEALIRW + Sbjct: 422 RNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWES 481 Query: 497 PDQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACAQNKAWQDAGLAPIPVAVNLSPRQLDQ 556 P QGMVPPDRFIPFAEEAGLIVPIGEWVLRTAC QN+AWQ AGL PIPVAVNLSPRQL+Q Sbjct: 482 PQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQ 541 Query: 557 NLPEFVRQVLAESCLAAAWLELEITENVVMKDAEKTVATLNALKRLGLQVSVDDFGTGYS 616 +LP+FV VLA+S LAA+WLELEITENVVMKDAEK+VATL+ALKRLGLQ+SVDDFGTGYS Sbjct: 542 SLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYS 601 Query: 617 SLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLGHILNLRVIAEGVEDQEQYNF 676 SLSYLRRFPVDALKIDKSFVRDI RDADSAAIVKA+ISL HILNLRVIAEGVED+EQ+ F Sbjct: 602 SLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAF 661 Query: 677 LLENGCDEVQGYLLGKPMPAEDFT 700 L EN CDEVQGY GKPM FT Sbjct: 662 LKENACDEVQGYYFGKPMEVAVFT 685 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 509 bits (1311), Expect = e-145, Method: Compositional matrix adjust. Identities = 280/572 (48%), Positives = 367/572 (64%), Gaps = 8/572 (1%) Query: 139 DVTARKTADQALLEAERRYRSI--FENAIEGVFQSTPDGGYIAVNPALARIYGYKSADDL 196 D+ AR+ A++AL R+++I NAI + PD VNPA ARI GY S + + Sbjct: 379 DIKARRLAERAL---RLRHQAIEASANAILILSAQEPDYTIEYVNPAFARITGYSSTEAV 435 Query: 197 IVSLRDIKHQLYVEPERRTEFMRMMAEFGSVSNFESRVYRRDGEIIWISENARAVRDEAG 256 S+R ++ + +PE M E N R Y +DG +W VRDE+G Sbjct: 436 GKSIRFLEGR-DSDPEGFETVWIAMRE-QREHNVVLRNYHKDGSELWNDFYIAPVRDESG 493 Query: 257 TLMFYEGTVEAITERKLYEAEIRHQATHDALTGLPNRTMLHNHLQRAIQSTRQKGTLTAV 316 + + + IT K YEAE+ QA+ D LTGL NR ML +HL++AI ++ V Sbjct: 494 EVTHFVAALYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWV 553 Query: 317 VFVDLDQFKFINDSLGHQVGDELLKTVAQRLVSCVRETDMVARQGGDEFVLVLQNQPDEN 376 VFVDLD+FKF+ND+LGH+ GD LL+TVA RL + VRE D VAR GGDEF+++L Q + Sbjct: 554 VFVDLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELT 613 Query: 377 RITEVLQRILTAVARPWVVGDREFHVTCSIGVSRCPTDGRDVETLLKNADSAMYKAKELG 436 T LQRI+ AVA P +G +TCS+GV+ P DG D ETL+K+AD AMY+ K+ G Sbjct: 614 LDTRSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTG 673 Query: 437 RNNFQYFAGWMDAQVSNRLEMLVSLRRALDRDELLLYYQPKISLASGRIIGAEALIRWIT 496 RNNFQ++ M+ Q RL + LR ALD E LL+YQPK+ LASGR+IG+EALIRW Sbjct: 674 RNNFQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQH 733 Query: 497 PDQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACAQNKAWQDAGLAPIPVAVNLSPRQ-LD 555 P GMV P RFI AEE GLI+PIG WVLR AC Q + WQ G A I V+VNLS Q L Sbjct: 734 PQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQ 793 Query: 556 QNLPEFVRQVLAESCLAAAWLELEITENVVMKDAEKTVATLNALKRLGLQVSVDDFGTGY 615 ++L + VL ++ L LELE+TE+++M D E + L LK+LG+++S+DDFGTGY Sbjct: 794 KDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGY 853 Query: 616 SSLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLGHILNLRVIAEGVEDQEQYN 675 SSL+YL+RFP+D LKID+SFVRDI DAD AAI ++ISL L LRVIAEGVE EQ + Sbjct: 854 SSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLD 913 Query: 676 FLLENGCDEVQGYLLGKPMPAEDFTRRLELEK 707 +L GCDE+QGYL P+PA+ F + L ++ Sbjct: 914 YLRHYGCDEIQGYLFSPPVPADKFEQILRQDR 945 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 440 bits (1132), Expect = e-124, Method: Compositional matrix adjust. Identities = 262/671 (39%), Positives = 391/671 (58%), Gaps = 40/671 (5%) Query: 50 FVSEGCLELTGYQPEQLLFNHRISYED----ITHPEDREKVRRTIQSCLDGKRRFELEYR 105 +++ L+ TGY E+ L + +D I HPED ++R + C+DG++R ++E R Sbjct: 375 WINHAVLDYTGYTVEECL-----AMDDFIGTIAHPEDEPRIRAELARCMDGQKREDVELR 429 Query: 106 IVRADGEVRWVWERGVGIYNAAGTLDALEGYVQDVTARKTADQALLEAERRYRSIFENAI 165 VR DG V W+ I++A G +D+T RK +EAE R ++ ++ Sbjct: 430 CVRKDGSVFWLALSCAPIHDAQGRFTGFRTSSRDITDRKQ-----IEAELRISAVAFDSR 484 Query: 166 EGVFQSTPDGGYIAVNPALARIYGYKSADDLIVSLRDIKHQLYVEPERRTE--FMRMMAE 223 EG+ + + + VN A I GY SA++++ KH + +R F M Sbjct: 485 EGMLITDANSKILRVNKAFTEITGY-SAEEIVG-----KHPNLLRSDRHGPAFFQEMRES 538 Query: 224 FGSVSNFESRVY--RRDGEII--WISENARAVRDEAGTLMFYEGTVEAITERKLYEAEIR 279 ++ ++ R++GE+ W++ +A V+++ + Y T I++RKL E IR Sbjct: 539 IRRFGKWQGEIWDRRKNGEVYPEWLTISA--VKNKDQQVTHYVSTHHDISDRKLAEERIR 596 Query: 280 HQATHDALTGLPNRTMLHNHLQRAIQSTRQKGTLTAVVFVDLDQFKFINDSLGHQVGDEL 339 A DALT LPNRT+L + L++AI + + T A++F+DLD FK +ND++GH+ GD L Sbjct: 597 ELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTIGHEKGDTL 656 Query: 340 LKTVAQRLVSCVRETDMVARQGGDEFVLVLQN-----QPDENRITEVLQRILTAVARPWV 394 LK VAQRL++ VRE D VAR GGDEFV+VL+ Q + + ++ILTA+ + Sbjct: 657 LKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKILTALGSTYQ 716 Query: 395 VGDREFHVTCSIGVSRCPTDGRDVETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNR 454 + D E+ T SIG + V+ L+K AD AMYKAKE GRN ++F M V R Sbjct: 717 LDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQTAVLER 776 Query: 455 LEMLVSLRRALDRDELLLYYQPKISLASGRIIGAEALIRWITPDQGMVPPDRFIPFAEEA 514 + LR+A+D ++LLL+YQP++ + GR+ GAE L+RW P +GMVPP FIP AEE Sbjct: 777 ATLEAGLRKAIDGNQLLLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEET 835 Query: 515 GLIVPIGEWVLRTACAQNKAWQ-DAGLAPIPVAVNLSPRQLDQNLPEFVRQVLAESCLAA 573 GLI+ +G WVL TAC Q W +A + +AVN+S QL + P+FV+ VL Sbjct: 836 GLILSLGNWVLETACRQLATWAAQPQMADLSIAVNVSAPQLRE--PDFVQTVLDTIERTG 893 Query: 574 A---WLELEITENVVMKDAEKTVATLNALKRLGLQVSVDDFGTGYSSLSYLRRFPVDALK 630 A L+LE+TE++++ + E + + ALK G++ S+DDFGTGYSSLSYL+R P++ LK Sbjct: 894 ANPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLK 953 Query: 631 IDKSFVRDIARDADSAAIVKAVISLGHILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLL 690 ID+SFVRD+ D + A+I K +++L L L VIAEGVE Q +FL + GC QGY Sbjct: 954 IDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFF 1013 Query: 691 GKPMPAEDFTR 701 +P+ E F + Sbjct: 1014 CRPVTVEAFEK 1024 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 410 bits (1055), Expect = e-115, Method: Compositional matrix adjust. Identities = 232/560 (41%), Positives = 333/560 (59%), Gaps = 15/560 (2%) Query: 160 IFENAIEGVFQSTPDGGYIAVNPALARIYGYKSADDLIVSLRDIKHQLYVEPERRTEFMR 219 I E V P G + N +++GY + + + R++ LY + Sbjct: 127 ILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAIG---RNVLF-LYENEDEEDSLHD 182 Query: 220 MMAEFGSVSNFESRVYRRDGEIIWISENARAVRDEAGTLMFYEGTVEAITERKLYEAEIR 279 + E G E R ++ GE+ W S + +RD+ G + G + ITERK E I Sbjct: 183 LFLEHGG-REMEVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEKLIH 241 Query: 280 HQATHDALTGLPNRTMLHNHLQRAIQSTRQKGTLTAVVFVDLDQFKFINDSLGHQVGDEL 339 H A +DALTGLPNRT+L + +A++ R + V+F+DL++FK IND+LGH GD L Sbjct: 242 HLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDML 301 Query: 340 LKTVAQRLVSCVRETDMVARQGGDEFVLVLQNQPDENRITEVLQRILTAVARPWVVGDRE 399 L VA RL +RE D+VAR G DEF + L D++ V Q++L P+ + E Sbjct: 302 LVEVAMRLRRALREQDVVARLGADEFAVALFG-IDKDSPAYVAQKLLALFDDPFFIDGHE 360 Query: 400 FHVTCSIGVSRCPTDGRDVETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNRLEML- 458 V SIGVS P + D ETLL+ AD AMY+AK+ G NN +A + + N L++L Sbjct: 361 LRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLLR 420 Query: 459 --VSLRRALDRDELLLYYQPKISLASGRIIGAEALIRWITPDQGMVPPDRFIPFAEEAGL 516 L A + +ELLLYYQPK+ + + I GAEAL+RW P++G++ P FIP AEE GL Sbjct: 421 IETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIPIAEETGL 480 Query: 517 IVPIGEWVLRTACAQNKAWQDAGLAPIPVAVNLSPRQLDQNLPEFVRQVLAESCLAAAWL 576 IV + +WVL C Q +AW+DAGL PI +A+N++ R+ ++LP+ V L L+ WL Sbjct: 481 IVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREFTRSLPDRVGAALERHMLSGEWL 540 Query: 577 ELEITENVVMKDAEKTVATLNALKRLGLQVSVDDFGTGYSSLSYLRRFPVDALKIDKSFV 636 ELEITE+++M ++ ++ + + LG+ VS+DDFGTGYSSLSYL+RFP++ LKID+SF Sbjct: 541 ELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKRFPINTLKIDRSFT 600 Query: 637 RDIARDADSAAIVKAVISLGHILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLLGKPMPA 696 I D + AI A+I + L +VIAEGVED++Q+NFL E GC+E+QGY+ +P+PA Sbjct: 601 MGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCNELQGYIFSRPVPA 660 Query: 697 EDF------TRRLELEKKAA 710 DF R+L LE +A Sbjct: 661 PDFHAMLLEGRKLALESASA 680 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 382 bits (981), Expect = e-107, Method: Compositional matrix adjust. Identities = 206/437 (47%), Positives = 281/437 (64%), Gaps = 6/437 (1%) Query: 277 EIRHQATHDALTGLPNRTMLHNHLQRAI-QSTRQKGTLTAVVFVDLDQFKFINDSLGHQV 335 E+ HD LT LPNRT+L + L +AI ++ R++G A++F+DLD FK INDSLGH V Sbjct: 261 ELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHF-ALMFMDLDGFKAINDSLGHHV 319 Query: 336 GDELLKTVAQRLVSCVRETDMVARQGGDEFVLVLQ-NQPDENRITEVLQRILTAVARPWV 394 GD LL VAQRL S R D VAR GGDEFV+++ N P++ V ++++ AV +P+ Sbjct: 320 GDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPEDA--MGVAEKLVEAVNQPFK 377 Query: 395 VGDREFHVTCSIGVSRCPTDGRDVETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNR 454 V E V+ S+G++ P DG L+ NAD+AMY K GRN + +F M+A + Sbjct: 378 VDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQQ 437 Query: 455 LEMLVSLRRALDRDELLLYYQPKISLASGRIIGAEALIRWITPDQGMVPPDRFIPFAEEA 514 L+ L LR AL+R + L YQPK SL +G ++GAEAL+RW P G+V PD+FI AE + Sbjct: 438 LQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERS 497 Query: 515 GLIVPIGEWVLRTACAQNKAWQDAGLAPIPVAVNLSPRQL-DQNLPEFVRQVLAESCLAA 573 GLI+PIG WVL AC Q + W G ++VNLS Q D +L E VR L L Sbjct: 498 GLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPP 557 Query: 574 AWLELEITENVVMKDAEKTVATLNALKRLGLQVSVDDFGTGYSSLSYLRRFPVDALKIDK 633 L LE+TE+ M D E ++ TL + LG+++S+DDFGTGYSSL YL+R P + LKID+ Sbjct: 558 PCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDR 617 Query: 634 SFVRDIARDADSAAIVKAVISLGHILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLLGKP 693 FVR++ +D + AAIV A+++LG LNLR++AEGVE EQ +FL GCD +QGYL+G+P Sbjct: 618 GFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRP 677 Query: 694 MPAEDFTRRLELEKKAA 710 M E F + + K+A Sbjct: 678 MTPELFLQTMRERHKSA 694 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,131,724 Number of extensions: 86204 Number of successful extensions: 392 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 312 Number of HSP's successfully gapped: 47 Length of query: 711 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 619 Effective length of database: 1,184,250 Effective search space: 733050750 Effective search space used: 733050750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)