BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0009.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 613 e-176 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 213 2e-56 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 189 4e-49 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 102 6e-23 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 102 7e-23 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 613 bits (1580), Expect = e-176, Method: Compositional matrix adjust. Identities = 298/459 (64%), Positives = 365/459 (79%), Gaps = 4/459 (0%) Query: 1 MRHSIIARLVMMFAAAALVIFSLIGGVLYAVVKNELLRHERDGLVTTFNDIEYMVSRVGT 60 +R SI ARLV M AA+AL+IF+L G VLY V+K ELLRHERDGLVTT NDI Y + R G Sbjct: 8 LRQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISYQIGRAGN 67 Query: 61 VERWARVQTKLDTLTPADGKVRFWVFSDDPQFSYGKDLAELHDFDY---DPNNIGRLRIK 117 ERW RVQ KL TL+ AD V FW+ S D +F+YG DL+ L + N++GRL + Sbjct: 68 TERWGRVQAKLATLSQADQHVSFWISSSDARFTYG-DLSRLAPQAIRADEGNSVGRLHLP 126 Query: 118 GKEFPMHTLTKVLPAYLDRPAIRLTIGVDTEPYFETLRTFVIAMIGLSLAGIFLVILFGY 177 G+ +P+H +T+ LPA+ +RPA+ +T+GVD EPYFE RTF++A SL GI L ++ Y Sbjct: 127 GRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVLAVISSY 186 Query: 178 WIARAGLQPLKKMSDKAQTLSPQTLSERLTVSALPDELSDLAQAFNGALDRMENAYKQLE 237 WIARAGL PL++MSD+AQ+ SP+ LS R+ V A+P ELS LA AFNGALDR+E+AY QLE Sbjct: 187 WIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIEDAYTQLE 246 Query: 238 AFNADVAHELRTPLANLIGETQVALSRERSAPEFEIVLQSNLEELDRLRSIINDMLFLAR 297 AFNADVAHELRTPLANLIGETQVAL+RERSA EF+ VLQSNLEELDR+RSI+NDMLFLAR Sbjct: 247 AFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLFLAR 306 Query: 298 CDQGEAATSLVYTAIADEVQKTIEFFEFVLDEMQMSVEVVGDVDAKSMIETALFRRALTN 357 DQGEAAT+ V +ADEVQKT++FFEFVLD+ M +EV GD +A +M+ETALF+RALTN Sbjct: 307 SDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETALFKRALTN 366 Query: 358 LLQNAIQHSPAGARITVSIDAQQQDVQVAVSNPGEPIEQRHLPRLFDRFYRVDSARRDFD 417 L+QNAIQHSP GA + + I Q++ V+VAVSNPG+PI++ HLPRLFDRFYRVDSAR+D + Sbjct: 367 LIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDSARQDIN 426 Query: 418 GVHGHGLGLAIVKAIALMHGGTAFASSRGGKTTIGFTVG 456 G HGHGLGLAIVKA+A MHGG AFASS+ G +IGFT+ Sbjct: 427 GSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSIGFTIA 465 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 213 bits (542), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 152/462 (32%), Positives = 239/462 (51%), Gaps = 27/462 (5%) Query: 4 SIIARLVMMFAAAALVIFSLIGGVLYAVVKNELLRHERDGLVTTFNDIEYMVSRVGTVER 63 S+ RLV +F A+VI + +G L + +L R + + L I ++V ++E Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLED 67 Query: 64 -WARVQTKLDTLTPADGKVRFWVFSDDPQFSYGKDLAELHDFDYDPNNIGRLRIKGKEF- 121 AR LDTLT D + +D G L E HD P I ++ + + Sbjct: 68 ILARRHLVLDTLTGHDQLLVRIARAD------GSTLLESHDPTRTPPGIDLSQVDIRAWD 121 Query: 122 --PMHTLTKVLPAYL-DRPAIR---------LTIGVDTEPYFETLRTFVIAMIGLSLAGI 169 L + Y+ +P R + D R+ V G+ G Sbjct: 122 SIQSWKLGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGV---GA 178 Query: 170 FLVILFGYWIARAGLQPLKKMSDKAQTLSPQTLSERLTVSALPDELSDLAQAFNGALDRM 229 L IL Y++ R+GL+PL+ M+ + + +S L RL + A P EL ++ ++FN LDR+ Sbjct: 179 LLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRL 238 Query: 230 ENAYKQLEAFNADVAHELRTPLANLIGETQVALSRERSAPEFEIVLQSNLEELDRLRSII 289 +++QL F+AD+AH++RTPL NL+ +TQVAL + R E++ +L SN+EE +RL ++ Sbjct: 239 HRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMM 298 Query: 290 NDMLFLARCDQGEAATSLVYTAIADEVQKTIEFFEFVLDEMQMSVEVVGDVDAKSMIETA 349 MLFLAR + +A E+ + ++FE + DE Q+ + V G + + Sbjct: 299 ESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQG--AGRLQADAT 356 Query: 350 LFRRALTNLLQNAIQHSPAGARITVSIDAQQQDVQVAVSNPGEPIEQRHLPRLFDRFYRV 409 L RRA+ NL+ NAI+++PA I + +DA ++++VSNPG I L RLFDRFYR Sbjct: 357 LLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRA 416 Query: 410 DSARRDFDGVHGHGLGLAIVKAIALMHGGTAFASSRGGKTTI 451 D +R GLGLAIV++I ++HGG A +S G+ T+ Sbjct: 417 DPSR--SSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTV 456 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 189 bits (480), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 103/258 (39%), Positives = 158/258 (61%), Gaps = 8/258 (3%) Query: 185 QPLKKMSDKAQTLSPQTLSERLTVSALPDELSDLAQAFNGALDRMENAYKQLEAFNADVA 244 QPL+ MS +A ++P LS RL +P EL +A +FN LDR+E Y L F+ D+A Sbjct: 200 QPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLA 259 Query: 245 HELRTPLANLIGETQVALSRERSAPEFEIVLQSNLEELDRLRSIINDMLFLARCDQGEAA 304 HE+RTP+ L+G++QVAL + RS E+E VL+SN+EEL RL I+ ++LFLA+ D A Sbjct: 260 HEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENILFLAQADHAALA 319 Query: 305 TSLVYTAIADEVQKTIEFFEFVLDE--MQMSVEVVGDVDAKSMIETALFRRALTNLLQNA 362 +A+E+ + ++FE + DE M+ V+ G A + ++RR ++NL+ NA Sbjct: 320 VERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLI----MWRRVVSNLVINA 375 Query: 363 IQHSPAGARITVSIDAQQQDVQVAVSNPGEPIEQRHLPRLFDRFYRVDSARRDFDGVHGH 422 +++ A + I + ++ + ++ V N E + + + R+FDRFYR D +R F + Sbjct: 376 VRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRSRSAF--TESN 433 Query: 423 GLGLAIVKAIALMHGGTA 440 GLGLAIVKAI +HGGTA Sbjct: 434 GLGLAIVKAIMALHGGTA 451 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 102 bits (254), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 83/273 (30%), Positives = 134/273 (49%), Gaps = 21/273 (7%) Query: 178 WIA-RAGLQPLKKMSDKAQTLSPQTLSERLTVSALPDELSDLAQAFNGALDRMENAYKQL 236 W A R L+PL ++ + ++ +P LS + + E+ L QA NG + R++ Sbjct: 191 WFAVRFVLRPLMQLKGEVESRAPTDLSG-FDTALVHKEMRPLVQAMNGYMGRLQALISAQ 249 Query: 237 EAFNADVAHELRTPLANLIGETQVALSRER----SAPEFEIVLQSNLEELDRLRSIINDM 292 F AD +H+LRTPL L ++++ L R A E +++ D + N + Sbjct: 250 RRFIADASHQLRTPLTVLKTQSELTLRELRREQVDAAELRDLVEGMARTTDAAVHLANRL 309 Query: 293 LFLARCDQGEAATSLVYTAIADEV-QKTIEFF-EFVLDEMQMSVEVVGDVDAKSMIETAL 350 L LAR + G A + +++D Q +E + V E+ ++ E GDV ++ L Sbjct: 310 LTLARAEHGAAEGGTMPLSLSDAARQVALELSQQAVSREIGLAFEEEGDVSIEA--NALL 367 Query: 351 FRRALTNLLQNAIQHSPAGARITVSIDAQQQDVQVAV-------SNPGEPIEQRHLPRLF 403 + NLL NAI+++PAG +T+ + AQ Q V S PG P +R ++F Sbjct: 368 LHELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAERE--KVF 425 Query: 404 DRFYRVDSARRDFDGVHGHGLGLAIVKAIALMH 436 + FYR + + G G GLGL+IV+ IA MH Sbjct: 426 EPFYRAGATQHVNPG--GTGLGLSIVREIAAMH 456 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 102 bits (254), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 92/296 (31%), Positives = 148/296 (50%), Gaps = 20/296 (6%) Query: 158 VIAMIGLSLAGIFLVILFGYWIARAGLQPLKKMSDKAQTLSPQTLSERLTVSALPDELSD 217 +IA++ + LA +V + G +PL +++ L+ + R+ V + DE+ Sbjct: 176 IIAVVAVMLAAFLVVRISGVLT-----RPLTRVARATHRLASGDYAIRVEVES-GDEVGM 229 Query: 218 LAQAFNGALDRMENAYKQLEAFNADVAHELRTPLANLIGETQVALSRERSAPEFEIVLQS 277 LAQ FN +E K F ADV+HELRTPLA L G+ + AL P+ + L+S Sbjct: 230 LAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLE-ALEDGVLQPDAQ-TLRS 287 Query: 278 NLEELDRLRSIINDMLFLARCDQGEAATSLVYTAIADEVQKTIEFFE--FVLDEMQMSVE 335 E++ L ++ D+ L+ D G +A ++ + + F M++S E Sbjct: 288 LKSEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCE 347 Query: 336 VVGDVDAKSMIETALFRRALTNLLQNAIQHSPAGARITVSIDAQQQDVQVAV--SNPGEP 393 + + + A ++ L+NL +N+++++ AG R+ V QQQ + S PG P Sbjct: 348 LPASMPVHG--DAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFDDSEPGVP 405 Query: 394 IEQRHLPRLFDRFYRVDSARRDFDGVHGHGLGLAIVKAIALMHGGT--AFASSRGG 447 E + RLF+RF+RV+++R G G GLGLAI + I H GT A AS GG Sbjct: 406 PEV--MARLFERFFRVEASRNRASG--GAGLGLAICRRIVDAHQGTIGASASPLGG 457 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,365,552 Number of extensions: 56316 Number of successful extensions: 325 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 29 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)