BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0007.p (85 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 150 3e-38 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 86 5e-19 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 82 8e-18 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 150 bits (378), Expect = 3e-38, Method: Composition-based stats. Identities = 71/74 (95%), Positives = 72/74 (97%) Query: 1 MTTMCALLSGLPLMLGHGAGSELRRPLGYAMVGGLVLSQALTLFTTPVVYLYLDRAHYWY 60 MTTMCALLSGLPLMLGHGAGSELRRPLGYAMVGGL+LSQALTLFTTPVVYLYLDRAHYWY Sbjct: 972 MTTMCALLSGLPLMLGHGAGSELRRPLGYAMVGGLILSQALTLFTTPVVYLYLDRAHYWY 1031 Query: 61 MNKKEARAARKAAR 74 M KKEAR ARKAAR Sbjct: 1032 MRKKEARQARKAAR 1045 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 86.3 bits (212), Expect = 5e-19, Method: Composition-based stats. Identities = 44/75 (58%), Positives = 54/75 (72%), Gaps = 6/75 (8%) Query: 1 MTTMCALLSGLPLMLGHGAGSELRRPLGYAMVGGLVLSQALTLFTTPVVYLYLDRAHYW- 59 MTTM ALL +PL +GHG G+ELR+PLG ++VGGLV+SQ LTL+TTPVVYLY++R W Sbjct: 1004 MTTMAALLGAIPLAIGHGDGAELRQPLGISIVGGLVVSQLLTLYTTPVVYLYMERLSSWS 1063 Query: 60 -----YMNKKEARAA 69 NK E AA Sbjct: 1064 KRRFGRKNKTETSAA 1078 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 82.0 bits (201), Expect = 8e-18, Method: Composition-based stats. Identities = 40/54 (74%), Positives = 44/54 (81%) Query: 1 MTTMCALLSGLPLMLGHGAGSELRRPLGYAMVGGLVLSQALTLFTTPVVYLYLD 54 MTTM ALL LPLMLG G GSELR+PLG MVGGL++SQ LTLFTTPV+YL D Sbjct: 962 MTTMAALLGALPLMLGTGVGSELRQPLGITMVGGLLVSQVLTLFTTPVIYLGFD 1015 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 231,551 Number of extensions: 7949 Number of successful extensions: 57 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 3 Length of query: 85 Length of database: 1,619,870 Length adjustment: 55 Effective length of query: 30 Effective length of database: 1,359,445 Effective search space: 40783350 Effective search space used: 40783350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 123 (52.0 bits)