BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0003.p (109 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 111 2e-26 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 52 1e-08 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 111 bits (277), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 63/107 (58%), Positives = 75/107 (70%), Gaps = 4/107 (3%) Query: 1 MTTSLTPKRST--LLISVAVLVALGGGAWSLFHRADAVAASKEQAQQAPSISVTTVQVQE 58 M+TS TP R + + A L+ G G W+ RA A A + QA+ P +SVT VQE Sbjct: 1 MSTSPTPPRRSRLAIALAAALLMAGIGGWTHLQRASA--ADQPQAKAPPPVSVTLASVQE 58 Query: 59 RDIPLYLSGVGTVTANASVVVKVRIDGQLDKVGFTEGQDVKAGQLLA 105 RD+P YL+GVGTVTANASVVVK RIDGQL+KVGF EG+DVK GQLLA Sbjct: 59 RDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLA 105 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 52.0 bits (123), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 24/47 (51%), Positives = 35/47 (74%) Query: 59 RDIPLYLSGVGTVTANASVVVKVRIDGQLDKVGFTEGQDVKAGQLLA 105 +D+ ++L+G+G VT A+ V+ R+DGQL K+ + EGQ VKAG LLA Sbjct: 42 QDVNVFLNGLGAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLA 88 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 320,057 Number of extensions: 11125 Number of successful extensions: 67 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 2 Length of query: 109 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 37 Effective length of database: 1,278,950 Effective search space: 47321150 Effective search space used: 47321150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 124 (52.4 bits)