BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0317.p (775 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G ... 1274 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 221 2e-58 >gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G [Herbaspirillum seropedicae SmR1] Length = 772 Score = 1274 bits (3298), Expect = 0.0, Method: Compositional matrix adjust. Identities = 656/776 (84%), Positives = 706/776 (90%), Gaps = 5/776 (0%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKVGTYIPHFCYHKKLSIAANCRMCLVDVEKAPKPLP 60 MVEIEIDGKKVEVQEGSMVMDAANK+GTYIPHFCYHKKLSIAANCRMCLV+VEKAPKPLP Sbjct: 1 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP 60 Query: 61 ACATPVTAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGSSS 120 ACATPV AGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYG SS Sbjct: 61 ACATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS 120 Query: 121 SRYEEEKRVVPPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 SRY+EEKRVV PKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF Sbjct: 121 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 Query: 181 VGNTVNSELSGNMIDLCPVGALTSKPFRYAARTWELSRRKSVSPHDSLGANLIVQVKGGK 240 VG TV+SELSGNMIDLCPVGALTSKPFRY+ARTWELSRRKSVSPHDS+GANLIVQVK GK Sbjct: 181 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK 240 Query: 241 VMRVLPLENEDINECWISDKDRFAYEALDSEERLTKPMLKQDGKWQEVEWQVALEYVAHG 300 VMRVLP ENE++NECWISDKDRFAYEALDSE+RLTKPMLKQDGKWQEV+WQ ALEYVAHG Sbjct: 241 VMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQDGKWQEVDWQTALEYVAHG 300 Query: 301 LRNIRHEHGASAIAALGTPYSTLEELTLLQKVVRGLGSENIDFRLRQSDFALDHQVNPWL 360 LRNIRHEHGA AIAALGTPYSTLEEL+LLQK+VRG+GSENIDFRLRQSDF LD Q+ PWL Sbjct: 301 LRNIRHEHGADAIAALGTPYSTLEELSLLQKIVRGVGSENIDFRLRQSDFGLDGQIKPWL 360 Query: 361 GMSIVEFSELNRALIIGSFLRKDHPLLTARLRQAVKSGAKISILHAADDDLLMPVANKII 420 GMSIVEFS+LNR +IGSFLRKDHPLL+ARLRQ+ KSGAK+S+LHA DDDLLMPVANKII Sbjct: 361 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPVANKII 420 Query: 421 AAPSAWLAALSGIVAAVAQAKGVAAPAGFENIEISPEAKQTAASLLSGASKAVLLGNAAA 480 AAPSAWL+AL+ + AVA+AKGVAAP+ + + S AK TAASLLSG +K V LGNAA Sbjct: 421 AAPSAWLSALAEVAVAVAEAKGVAAPSAYAGVTASDAAKATAASLLSGDAKGVFLGNAAV 480 Query: 481 QHPQASQLHAAAQWIAEQTGAKLGYLTEGGNAVGGYLANAIPGKDGANALQAFAQPRKAY 540 QHPQA+QLHAAAQWIAEQTGAKLG LTEGGNAVGGYLANA+ A FAQPRKAY Sbjct: 481 QHPQAAQLHAAAQWIAEQTGAKLGVLTEGGNAVGGYLANAV--PAAGKAAAVFAQPRKAY 538 Query: 541 LLLNAEPELDSHDSQAATAALKQAEMVVVMSAFKHGADYADVLLPIAPFTETAGTFVNAE 600 LLLNAEPELDS+D QAA+AALKQA+MVVVMSAFKHGADYADVLLPIAPFTET+GTFVN E Sbjct: 539 LLLNAEPELDSYDPQAASAALKQADMVVVMSAFKHGADYADVLLPIAPFTETSGTFVNTE 598 Query: 601 GRAQSFNGTVKPLGDARPAWKVLRVLGNLLGLPNFDYETSESIRTEIIGAETDLSARLNN 660 GRAQSFNGTVKP GDARP WKVLRVLGNLL L NF+YETSESIR E++G ETDLSARLNN Sbjct: 599 GRAQSFNGTVKPAGDARPGWKVLRVLGNLLELENFEYETSESIRDELLGKETDLSARLNN 658 Query: 661 IAKLAPQVGNISANGA-LERLADVPIHFADAIARRSPALLQTQDGQAPKAWLSAALSQKL 719 +A + Q+ +S A +ER+ADVPI+FAD IARRS LL+T+DGQAP+AWLSAAL+ KL Sbjct: 659 LASV--QLRAVSTAAAEVERVADVPIYFADPIARRSAPLLETRDGQAPRAWLSAALASKL 716 Query: 720 GIAAGDRVSVKQGQGSALLTAGIDKNLPENVVRVAAAHASTADLGSMFGAIVVEKA 775 G+A GD+V VKQG GSA LTA IDK LPENVVRVAAAHASTA LG MFGAIVVEKA Sbjct: 717 GVAEGDQVKVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA 772 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 221 bits (562), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 209/771 (27%), Positives = 329/771 (42%), Gaps = 109/771 (14%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKVGTYIPHFCYHKKLSIAANCRMCLVDVEKAPKPL- 59 MV +EIDG V V G+ VM A+ + G +P C L +CR+CLV++EK + + Sbjct: 19 MVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPFGSCRLCLVEIEKDGRKMK 78 Query: 60 --PA-CATPVTAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLA--V 114 PA C TP GM V+T + K + ++GVME + +HPLDC C G C+LQD+A V Sbjct: 79 GYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDCLTCPTNGNCELQDMAGVV 138 Query: 115 GYGSSSSRYEEEKRVVPPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEH 174 G YE E KD + ++CI C RCVR +E G L + GRG Sbjct: 139 GLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRACEETQGTFALTINGRGFE 198 Query: 175 SEIT-----TFVGNTVNSELSGNMIDLCPVGALTSKPFRYAARTWELSRRKSVSPHDSLG 229 S ++ F+ + S G ++ CP LT K + E S+ + + + +G Sbjct: 199 SRVSAGQMEAFMESECVS--CGACVEACPTATLTEKTVIMLGQA-EHSKVTTCA-YCGVG 254 Query: 230 ANLIVQVKGGKVMRVLPLENEDINECWISDKDRFAYEALDSEERLTKPMLKQ--DGKWQE 287 + ++KG +V+R++P ++ N+ K RFA+ ++R+ PM+++ + W+E Sbjct: 255 CSFKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRILNPMIRKSINDPWRE 314 Query: 288 VEWQVALEYVAHGLRNIRHEHGASAIAALGTPYSTLEELTLLQKVVR-GLGSENIDFRLR 346 V W+ AL Y A R I+ +HG +I + + T EE L+QK+VR G+ N+D R Sbjct: 315 VSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCAR 374 Query: 347 QSDFALDHQVNPWLGMS--IVEFSELNRA---LIIGSFLRKDHPLLTARLRQAVKSGAKI 401 + + LG S F + ++ LI+G+ HP+ +++++ V+ GAK+ Sbjct: 375 VCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVFASQIKRRVRQGAKL 434 Query: 402 SILHAADDDL-------------LMPVANKIIAAPSAWLAALSGIV--AAVAQAKGVAAP 446 ++ ++ L P N I A + G+ A + + + + Sbjct: 435 IVIDPRTTEMVKSPHIQADYHLKLRPGTNTAIITALAHVILSEGLQDEAYIQERCDLQSY 494 Query: 447 AGFENIEI----SPEAK-----------QTAASLL-SGASKAVLLGNAAAQHPQASQLHA 490 ++ + SPEA + AA L +G + A+ G +H Q S Sbjct: 495 QDWKEFVLREDNSPEAMAAITGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVM 554 Query: 491 AAQWIAEQT------GAKLGYLTEGGNAVG----GYLANAIPGKDGANALQAFAQPRKAY 540 +A T G + L N G G + +PG + Q +A+ Sbjct: 555 GIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRNQFEQAW 614 Query: 541 -LLLNAEPEL--------------------------DSHDSQAATAALKQAEMVVVMSAF 573 + LN EP L ++Q AAL+ E +VV F Sbjct: 615 GVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIVVQDLF 674 Query: 574 -KHGADYADVLLPIAPFTETAGTFVNAEGRAQSFNGTVKPLGDARPAWKVLRVLGNLLGL 632 A YA V LP + F E GTF NAE R V P + W+V L LG Sbjct: 675 LNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVR-KVMPPKAGKSDWEVTVALAEALGY 733 Query: 633 PNFDYETSESIRTEIIGAETDLSARLNNIAKLAPQVGNISANGALERLADV 683 P Y I ++ IA L P +S + LE+L + Sbjct: 734 P-MPYNHPSEI--------------MDEIAALTPSFHGVSYD-KLEKLGSI 768 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,371,067 Number of extensions: 99641 Number of successful extensions: 380 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 372 Number of HSP's successfully gapped: 2 Length of query: 775 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 682 Effective length of database: 1,179,515 Effective search space: 804429230 Effective search space used: 804429230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)