BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0307.p (326 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 545 e-156 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 124 1e-29 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 122 5e-29 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 86 5e-18 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 74 2e-14 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 545 bits (1405), Expect = e-156, Method: Compositional matrix adjust. Identities = 265/325 (81%), Positives = 292/325 (89%) Query: 1 MRAIEITEFGGPDVLQLCERPLPQLKSGEILIKVHAAGVNRPDVFQRQGNYPVPPGASDL 60 MRAIEI EFGGP+VLQ CERP+P+LK+GE+LIKVHAAGVNRPDVFQR GNYPVPPGASDL Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 Query: 61 PGLEVAGEIVDGDLGGSAFKKGDLVCALVQGGGYAEYCAAPLAQCLPVPQGLNALQAAAL 120 PGLEVAGEIV GDLG S FK GDLVCALVQGGGYAE+CAAPLAQCLPVP+GL+AL+AAAL Sbjct: 61 PGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAAL 120 Query: 121 PENFFTVWSNVFERARLVPGETLLVQGGSSGIGVTAIQIAAALGHRVFATAGSDDKCRAC 180 PEN+FTVWSNVF+R +L ETLLVQGGSSGIGVTAIQIA ALGHRVFATAGS +KCRAC Sbjct: 121 PENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKCRAC 180 Query: 181 EELGAELAINYRTEDFEEVLKTATEGRGVDVILDMVGGDYLPREIKTLADDGRLVIIALL 240 EELGAE AINY+TEDFE V+K T GRGVDVILDMVGGDYLPREIK LADDGRLV IA L Sbjct: 181 EELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLVFIAQL 240 Query: 241 GGAKGQLDMSQILRRRLTVTGSTLRPRPVEFKAFIARQLQERVWPLLASGKVKPVIYQTF 300 GGAKGQLDM Q++RRRLT+TGSTLRPRPV FKA IA +L+E VWPL+ +GK++PVI+ F Sbjct: 241 GGAKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLREHVWPLIEAGKIRPVIHHRF 300 Query: 301 PLAQAAQAHALMESSAHVGKIMLTL 325 PL QAAQAHA+ME S H+GKIML L Sbjct: 301 PLEQAAQAHAMMEGSTHIGKIMLEL 325 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 103/330 (31%), Positives = 152/330 (46%), Gaps = 18/330 (5%) Query: 2 RAIEITEFGGPDVLQLCERPLPQLKSGEILIKVHAAGVNRPDVFQRQGNYPVPPGASDLP 61 +AI +++ GGP+V++ + + + +GE+ I+ A G+N D + R G YP P LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQP-----LP 57 Query: 62 ---GLEVAGEIVDGDLGGSAFKKGDLVC-ALVQGGGYAEYCAAPLAQCLPVPQGLNALQA 117 G E AG I G S FK GD V A G YAE P + +P ++ A Sbjct: 58 AGLGQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTA 117 Query: 118 AALPENFFTVWSNVFERARLVPGETLLVQGGSSGIGVTAIQIAAALGHRVFATAGSDDKC 177 AA+ TV + + + PG+T+L + GIG+ A Q A ALG + T SD+K Sbjct: 118 AAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKA 177 Query: 178 RACEELGAELAINYRTEDFEEVLKTATEGRGVDVILDMVGGDYLPREIKTLADDGRLVII 237 + GA INY+TE+F E +K T GRGV V+ D +G D + L G +V Sbjct: 178 ALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSF 237 Query: 238 ALLGGAKGQLDMSQILRRRLTVTGSTLRPRPVEFKAFIARQLQE----RVWPLLASGKVK 293 G +S++ R GS RP R + ++ ++ K+ Sbjct: 238 GNASGPVAPFGVSELASR-----GSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKIS 292 Query: 294 PVIYQTFPLAQAAQAHALMESSAHVGKIML 323 I Q + L AQAHA +E G +L Sbjct: 293 IEINQRYALKDVAQAHADLEGRKTTGSTIL 322 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 122 bits (305), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 111/353 (31%), Positives = 167/353 (47%), Gaps = 48/353 (13%) Query: 1 MRAIEITEFGGPDVLQLCERPLPQLKSGEILIKVHAAGVNRPDVFQRQGNYPVPPG---- 56 M+AI+ FGGP+VL+ + P+P+L+ GE+L++VHA G+N PD + R+G +PP Sbjct: 6 MKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPT 65 Query: 57 -------ASDLPGL--EVAGEIVDGDLGGSAFKKGDLVCALVQGG-GYAEYCAAPLAQCL 106 +D+ G+ VA ++ D +G + L G YA+Y + ++ Sbjct: 66 VAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIA 125 Query: 107 PVPQGLNALQAAALPENFFTVWSNVFE------------RARLVP--GETLLVQGGSSGI 152 PQ ++ QAAA P + T W + E R VP +T++V G + G+ Sbjct: 126 LKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGV 185 Query: 153 GVTAIQIAAALGHRVFATAGSDDKCRACEELGAELAINYRTEDFEEVLKTATEGRGVDVI 212 G A+Q+A G RV A GS +LGA+ I+Y E+VL VD++ Sbjct: 186 GHFALQLAKWKGARVIAI-GSGGHEAFLRDLGADEFIDYTRSAAEDVLSD------VDLV 238 Query: 213 LDMVGGDYLPREIKTLADDGRLVIIALLGGAKGQLDMSQILRRRLTVTGSTLRPRPVEFK 272 +D VGG R ++TL G L I LG + + RL V ST + R Sbjct: 239 IDAVGGPASGRFLRTLKRGGALFPIFPLGFSGAEEAA------RLGVNVSTTQVRSS--- 289 Query: 273 AFIARQLQERVWPLLASGKVKPVIYQTFPLAQAAQAHALMESSAHVGKIMLTL 325 QLQ+ + LL SG V+ I TF L A +AH GKI+L++ Sbjct: 290 ---GAQLQQ-LAGLLDSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSV 338 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 85.9 bits (211), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 92/336 (27%), Positives = 146/336 (43%), Gaps = 39/336 (11%) Query: 1 MRAIEITEFGGPDVLQLC---ERPLPQLKSGEILIKVHAAGVNRPDVFQRQG--NYPVPP 55 MRAI ++G P VL LC E+PLP G + + + AAGV D R G + V Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLP---PGHVRVAMQAAGVAPVDTKLRAGRLQHLVAL 57 Query: 56 GASDLPGLEVAGEIVDGDLGGSAFKKGDLVCALVQGGGYAEYCAA---PLAQCLPVPQGL 112 +PG + AG I G + G+ VC + G A P Q + P L Sbjct: 58 DLPKVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVL 117 Query: 113 NALQAAALPENFFTVWSNVFERARLVPGETLLVQGGSSGIGVTAIQIAAALGHRVFATAG 172 + QAAAL + + W + + A + G + + GS +G +Q+AA LG V A+ Sbjct: 118 SMQQAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCR 177 Query: 173 SDDKCRACEELGAELAINYRTEDFEEVLKTATEGRGVDVILDMVGGDYLPREIKTLADDG 232 S + LGA + Y ++ V R DV++D+VGG + + L G Sbjct: 178 SSN-VEYVAALGASRVLAYDRDELGRV-------RDQDVVIDLVGGAAHDQSYRMLRRGG 229 Query: 233 RLVIIALLGGAKGQLDMSQILRRRLTVTGSTLRPRPVEFKAFIA--RQLQERVWPLLASG 290 +LV + ++ +R R G ++R +A I + + ++V L G Sbjct: 230 QLVWL-----------VAAPIRERGEEFGVSVR------RALIVEDQSILQQVAALAQQG 272 Query: 291 KVKPVIYQTFPLAQAAQAHALMESSAHVGKIMLTLD 326 +P + P+ + A+AH +E+ V + L LD Sbjct: 273 VWRPAVSGVLPMQEIARAHQRIEAG-EVTRGRLVLD 307 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 81/296 (27%), Positives = 127/296 (42%), Gaps = 36/296 (12%) Query: 1 MRAIEITEFGGPDVLQLCERPLPQLKSGEILIKVHAAGVNRPDVFQRQGNYPVPPGASDL 60 M+A + EFG P L + + P+P G+IL+K A+GV D+ G++PV P + Sbjct: 5 MKAAVVREFGKP--LSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFI 62 Query: 61 PGLEVAGEIVDGDLGGSAFKKGDLV----------------------CALVQGGGY---- 94 PG E G + G K+GD V CA Q GY Sbjct: 63 PGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSVNG 122 Query: 95 --AEYCAAPLAQCLPVPQGLNALQAAALPENFFTVWSNVFERARLVPGETLLVQGGSSGI 152 AEY A +P L+ AA + TV+ + E + PGE +++ G G+ Sbjct: 123 SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKE-TEVRPGEWVVIS-GIGGL 180 Query: 153 GVTAIQIAAALGHRVFATAGSDDKCRACEELGAELAINYRTED-FEEVLKTATEGRGVDV 211 G A+Q A A+G V A +DK ++LGA+L ++ R + EV + G Sbjct: 181 GHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAHG--A 238 Query: 212 ILDMVGGDYLPREIKTLADDGRLVIIALLGGAKGQLDMSQILRRRLTVTGSTLRPR 267 ++ V + + L G + ++ L G L + + +R+TV GS + R Sbjct: 239 LVTAVSPKAMEQAFGFLRARGTMALVGLPPGDI-SLPVFNTVLKRITVRGSIVGTR 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,122,355 Number of extensions: 51312 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 8 Length of query: 326 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 241 Effective length of database: 1,217,395 Effective search space: 293392195 Effective search space used: 293392195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)