BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0304.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2... 479 e-136 gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2... 174 1e-44 gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 122 5e-29 >gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 344 Score = 479 bits (1232), Expect = e-136, Method: Compositional matrix adjust. Identities = 242/344 (70%), Positives = 278/344 (80%), Gaps = 8/344 (2%) Query: 1 MIDIIQPGLLTSVQDLGRPGHRRLGINPGGALNPLALSTANLLVGNDVSAAGLEITMGAC 60 MIDIIQPG+L SVQD+GR GHRRLGI+PGGALN LAL++ANLLVGN V AAGLEITMG Sbjct: 1 MIDIIQPGMLASVQDMGRYGHRRLGIHPGGALNTLALASANLLVGNPVDAAGLEITMGVA 60 Query: 61 ELRFTTDTRIALGGDDFDARLDDKFLAPYWSTPVRAGQTLRLAHVGRGGAQ-----GMRT 115 ELRFT TRIALGGDD A LD K +A WS PVRAG L+L+ + MRT Sbjct: 61 ELRFTRATRIALGGDDIGATLDGKPIAACWSIPVRAGSILKLSLAAADSPEQAPRHSMRT 120 Query: 116 CLAIAGGIDVPIILGSRSTDLKAGFGGHQGRALRKGDRLPLGDAGVKAT---GPAFGVRS 172 LA+AGGIDVP++LGSRSTDLKAGFGGHQGRAL++GD+LP+GDA A GPAFGVRS Sbjct: 121 YLAVAGGIDVPLVLGSRSTDLKAGFGGHQGRALKRGDKLPIGDAQAAAAAVHGPAFGVRS 180 Query: 173 PQWSGLSAQANDSSKLTTLRVLPGPEFEQFTVAARNTLWQDKWRITPNSNRMGYRLEGSE 232 P+WSGL+ +A++ S+ TLRVLP PEF+QF+ +AR LW D WR+TPNSNRMGYRLEG E Sbjct: 181 PRWSGLALRADEHSQHVTLRVLPAPEFDQFSKSAREALWSDTWRVTPNSNRMGYRLEGPE 240 Query: 233 LKRKQSRDLLSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGVVIAADMWKLAQAPLN 292 LKRKQ RD+LSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGV+I AD+WKLAQAPLN Sbjct: 241 LKRKQGRDMLSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGVIIGADLWKLAQAPLN 300 Query: 293 GLLQLQLCDLEQALHAWEEQQRYLAHVEQTIAALGWSRATQYRK 336 L+L+ C++ AL A EEQQRYL + T+AAL WSRAT Y+K Sbjct: 301 ATLRLEPCEMADALAAAEEQQRYLDSIRATVAALDWSRATLYKK 344 >gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 325 Score = 174 bits (440), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 121/318 (38%), Positives = 165/318 (51%), Gaps = 23/318 (7%) Query: 2 IDIIQPGLLTSVQDLGRPGHRRLGINPGGALNPLALSTANLLVGNDVSAAGLEITMGACE 61 I + QPGL TSVQD GR G+ LGI P GAL+ AL +ANLLVGN S + LE ++ + Sbjct: 3 IKVKQPGLATSVQDGGREGYYHLGIPPSGALDQYALRSANLLVGNHASTSALECSLLGPQ 62 Query: 62 LRFTTDTRIALGGDDFDARLDDKFLAPYWSTPVRAGQTLRLAHVGRGGAQGMRTCLAIAG 121 L F +D +A+ G R+D + VR+GQ L + G R+ LAIAG Sbjct: 63 LEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSFDYP----KAGARSYLAIAG 118 Query: 122 GIDVPIILGSRSTDLKAGFGGHQGRALRKGDRLPLGDAGVKATGPAFGVRSPQWSGLSAQ 181 GIDVP++L SRST GG QGR L K D LPLG A K A R+ S + Sbjct: 119 GIDVPVVLESRSTYALGALGGWQGRKLAKDDELPLGRASPK----AIEGRTLPLSLVPVY 174 Query: 182 ANDSSKLTTLRVLPGPEFEQFTVAARNTLWQDKWRITPNSNRMGYRLEGSELKRKQSRD- 240 ++ TLRV+PG + T A + D W + ++R+GYR +G + + + R+ Sbjct: 175 DREA----TLRVVPGLYIHRLTDEAVERFFDDTWTVGSEADRIGYRFKGGQAMQFRPREQ 230 Query: 241 ----------LLSHGVVPGVIQVPPSGQPIILMGDAQTTGGYPKIGVVIAADMWKLAQAP 290 ++ G IQ+P +PI+L+ DA + GGY IG VI+AD+ + Q Sbjct: 231 PFGAGSDPSNIVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQLQ 290 Query: 291 LNGLLQLQLCDLEQALHA 308 N Q LEQAL A Sbjct: 291 PNHKAQFVRVTLEQALEA 308 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 122 bits (305), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 105/300 (35%), Positives = 140/300 (46%), Gaps = 24/300 (8%) Query: 2 IDIIQPGLLTSVQDL-GRPGHRRLGINPGGALNPLALSTANLLVGNDVSAAGLEITMGAC 60 ++++ PG T+VQD GR G+ +G+ P G ++ AL N L+GN AAGLE+TM Sbjct: 455 VEVLAPGTQTTVQDHPGRIGYWAVGVPPSGPMDERALRLGNRLLGNAEEAAGLEVTMSGP 514 Query: 61 ELRFTTDTRIALGGDDFDARLDDKFLAPYWSTPVRAGQTLRLAHVGRGGAQGMRTCLAIA 120 LRF T + + G LD + AG TL+L + GA R+ LA+ Sbjct: 515 ILRFNTAAMVVVTGAVIPVLLDGVAQPMATVLHIAAGSTLKLGAISGAGA---RSYLAVR 571 Query: 121 GGIDVPIILGSRSTDLKAGFGGHQGRALRKGDRLPLGDAGVKATGPAFGVRSPQWSGLSA 180 GG+ VP LGSRST FGGH GRAL GD L L A A G P +A Sbjct: 572 GGVQVPTYLGSRSTFTLGQFGGHAGRALASGDILHLAPLAADAMTQA-GQTLP-----AA 625 Query: 181 QANDSSKLTTLRVLPGPE--FEQFTVAARNTLWQDKWRITPNSNRMGYRLEGSELKRKQS 238 + +RV+ GP E FT A T + W + NS+R G RL G K + Sbjct: 626 LYPALEAVRQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSSRTGVRLIGP--KPEWV 683 Query: 239 RDLLSH-GVVPGVIQVPPSG---------QPIILMGDAQTTGGYPKIGVVIAADMWKLAQ 288 RD G+ P I P P+IL D + GG+ VI AD+W++ Q Sbjct: 684 RDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGFVCPVTVIEADLWQIGQ 743 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,161,611 Number of extensions: 52103 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 3 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)