BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0295.p (463 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311081|ref|YP_003775173.1| high-affinity gluconate transpo... 707 0.0 gi|300310424|ref|YP_003774516.1| high-affinity gluconate permeas... 375 e-105 gi|300311416|ref|YP_003775508.1| gluconate transporter family pr... 247 2e-66 >gi|300311081|ref|YP_003775173.1| high-affinity gluconate transporter (GNT-III system) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 707 bits (1826), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/402 (91%), Positives = 382/402 (95%), Gaps = 3/402 (0%) Query: 1 MEAVQGSMLLIYALVAVIALIVLIAKFKMNPFIVLIVVSLILGLAVGMPMGAIVKSFETG 60 MEAVQGS LL+YALVAVIALIVLIAKFKMNPFIVLIVVSL+LGLAVGMPMG IVK+FETG Sbjct: 1 MEAVQGSALLVYALVAVIALIVLIAKFKMNPFIVLIVVSLVLGLAVGMPMGNIVKAFETG 60 Query: 61 VGNTLGHIALVVGLGTMLGKMMAESGGAERIATTMIGAFGEKNVHWAMMTVAFIVGLPVF 120 VGN LGHIALVVGLGTMLGKMMAESGGAERIA TMI AFGEKNVHWAMMTVAFIVGLPVF Sbjct: 61 VGNALGHIALVVGLGTMLGKMMAESGGAERIANTMIKAFGEKNVHWAMMTVAFIVGLPVF 120 Query: 121 FEVGFVLLIPIAFNVAKRTGTSMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYNADIGR 180 FEVGFVLL+PIAFNVAKRTGT+MVLVGIPMVAGLSVVHGLIPPHPAALLAVTAY+ADIGR Sbjct: 121 FEVGFVLLVPIAFNVAKRTGTNMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYSADIGR 180 Query: 181 TILFALIVGIPTAIIAGPIFGKLMSKVVIPNPDNPLIGQFVDQGANGGTKRELPGFGITL 240 TIL+ALIVGIPTAIIAGPIFGKL+SKVVIPNPDNPLI QFVD+G RELPGFGITL Sbjct: 181 TILYALIVGIPTAIIAGPIFGKLISKVVIPNPDNPLISQFVDEGKK---DRELPGFGITL 237 Query: 241 FTILLPVALMLIGSWADLFFTPKTFANDFLRLIGNSVIALLIATLVSFYTFGKLRGFNRE 300 FTILLPVALMLIGSWADLFF PKTFANDFLRLIGNSVIALLIATLVSF+TFG+ RGF + Sbjct: 238 FTILLPVALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFWTFGRARGFGAD 297 Query: 301 AILKFTNECLGPIASITLVVGAGAGFGRILMDGGVSKAIVGIATDAHLSPLLLGWFVAAL 360 ILKFTNECL PIASITLVVGAGAGFGRILMDGGVSKAIVGIATDAHLSPL+LGWFVAAL Sbjct: 298 QILKFTNECLAPIASITLVVGAGAGFGRILMDGGVSKAIVGIATDAHLSPLILGWFVAAL 357 Query: 361 IRVATGSATVAMTTACGIVAPIVMSVGGTRPELMVLATGAGS 402 IRVATGSATVAMTTACGIVAPIV +VGG RPELMVLATGAGS Sbjct: 358 IRVATGSATVAMTTACGIVAPIVSTVGGVRPELMVLATGAGS 399 >gi|300310424|ref|YP_003774516.1| high-affinity gluconate permease [Herbaspirillum seropedicae SmR1] Length = 450 Score = 375 bits (963), Expect = e-105, Method: Compositional matrix adjust. Identities = 206/398 (51%), Positives = 282/398 (70%), Gaps = 4/398 (1%) Query: 5 QGSMLLIYALVAVIALIVLIAKFKMNPFIVLIVVSLILGLAVGMPMGAIVKSFETGVGNT 64 + LL A++A++ALI++I + +++PFI L + S +LGL GMP+ IVKSF+ G G Sbjct: 6 HDATLLFNAVIAILALILMITRLRIHPFIALTITSGLLGLISGMPLPKIVKSFQDGFGGV 65 Query: 65 LGHIALVVGLGTMLGKMMAESGGAERIATTMIGAFGEKNVHWAMMTVAFIVGLPVFFEVG 124 LG + +V+GLGTMLGK+MAESGGA++IA T++ AFG + VHWAMM AF+VG+P+FFE+G Sbjct: 66 LGFVGIVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPLFFEIG 125 Query: 125 FVLLIPIAFNVAKRTGTSMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYNADIGRTILF 184 FVLLIP+ F VA+R+G + +G+PM+A LSV+HGL+PPHP LLA+ + ADIG+TI + Sbjct: 126 FVLLIPLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIGKTIFY 185 Query: 185 ALIVGIPTAIIAGPIFGKLMSKVVIPNPDNPLIGQFVDQGANGGTKRELPGFGITLFTIL 244 L+VG+PT IIAGP+FG +S+ V P L+ Q V + +LPGF TL T+L Sbjct: 186 GLLVGLPTVIIAGPMFGAFISRYVDIKPSEELMAQLVREPQT----TKLPGFATTLITVL 241 Query: 245 LPVALMLIGSWADLFFTPKTFANDFLRLIGNSVIALLIATLVSFYTFGKLRGFNREAILK 304 PV LML+ + D+ D + IGN ++ALL A L++ +TFG RGF R+ +LK Sbjct: 242 APVFLMLLKTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVLK 301 Query: 305 FTNECLGPIASITLVVGAGAGFGRILMDGGVSKAIVGIATDAHLSPLLLGWFVAALIRVA 364 F ++ L P A+I L++GAG GF ++L++ GV AI +A A +SPLLL WFVA +IRVA Sbjct: 302 FVDQSLAPTAAIVLIIGAGGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRVA 361 Query: 365 TGSATVAMTTACGIVAPIVMSVGGTRPELMVLATGAGS 402 TGSATVA T GIVAP+V + GT EL+VLATGAGS Sbjct: 362 TGSATVATITGAGIVAPLVALIPGTNKELLVLATGAGS 399 >gi|300311416|ref|YP_003775508.1| gluconate transporter family protein [Herbaspirillum seropedicae SmR1] Length = 456 Score = 247 bits (630), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 155/384 (40%), Positives = 228/384 (59%), Gaps = 7/384 (1%) Query: 20 LIVLIAKFKMNPFIVLIVVSLILGLAVGMPMGAIVKSFETGVGNTLGHIALVVGLGTMLG 79 ++VLI+ + PF+ +++ + + G+ G+P+ I K+F G G+ LG +++ LG MLG Sbjct: 27 IVVLISFLSIAPFLSILIGTFVAGIGAGLPLEDIAKAFSKGAGSLLGEAGIIIALGAMLG 86 Query: 80 KMMAESGGAERIATTMIGAFGEKNVHWAMMTVAFIVGLPVFFEVGFVLLIPIAFNVAKRT 139 +MAESG A+RI T++ + W M VA +VGLP+FFEVG V++ PI F +A+R+ Sbjct: 87 ALMAESGAADRIVNTLLRYARGSFIPWMMALVALVVGLPLFFEVGLVMMAPIIFVMARRS 146 Query: 140 GTSMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYNADIGRTILFALIVGIPTAIIAGPI 199 ++ V IP +AG++ +H L+PPHP L+AV+A +AD+G T+L IV IP IIAGP+ Sbjct: 147 ELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSALHADLGTTMLLGFIVAIPAVIIAGPL 206 Query: 200 FGKLMS-KVVIPNPDNPLIGQFVDQGANGGTKRELPGFGITLFTILLPVALMLIGSWADL 258 +G ++ ++ + PD IG+ A G + P F + L TILLPV LML + A + Sbjct: 207 YGNFIAPRLNLAEPDQ--IGKLFS--AREGQSQ--PSFLVALVTILLPVVLMLGRTVARI 260 Query: 259 FFTPKTFANDFLRLIGNSVIALLIATLVSFYTFGKLRGFNREAILKFTNECLGPIASITL 318 + TPKT + L G +IAL + L + G +G +R + L PIA + L Sbjct: 261 WVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSSRFEVGATLRRALPPIAGLLL 320 Query: 319 VVGAGAGFGRILMDGGVSKAIVGIATDAHLSPLLLGWFVAALIRVATGSATVAMTTACGI 378 +GAG G + L+ G+S I IA H+ +LL W +A +R ATGSATVA T GI Sbjct: 321 TIGAGGGLKQTLLSAGISDTITKIADGTHMPLILLAWIIAVALRQATGSATVATTATAGI 380 Query: 379 VAPIVMSVGGTRPELMVLATGAGS 402 VAP+V + T LM LA GAGS Sbjct: 381 VAPLVAGLSATHNSLMALAIGAGS 404 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.143 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,414,003 Number of extensions: 65585 Number of successful extensions: 358 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 350 Number of HSP's successfully gapped: 3 Length of query: 463 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 374 Effective length of database: 1,198,455 Effective search space: 448222170 Effective search space used: 448222170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)