BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0292.p (337 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [H... 554 e-159 gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [H... 271 5e-74 gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [H... 123 2e-29 >gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 496 Score = 554 bits (1428), Expect = e-159, Method: Compositional matrix adjust. Identities = 261/332 (78%), Positives = 292/332 (87%) Query: 4 FDTSDVSSSAHSCDTLIRNVFLVDGSGNDPVVADVAIAGGRIAAIGALGDWQAQQVMDGT 63 T+ + CD LIR+ ++DGSG +PV ADVA+ GR+AAIG L DWQA Q +D + Sbjct: 2 LSTTHAADGERRCDILIRHALVIDGSGAEPVAADVAVHKGRVAAIGKLDDWQAAQEIDAS 61 Query: 64 GKVLSPGFIDVHTHDDTNVIRMPDMFPKLSQGVTTVVVGNCGISASPVTLKGDPPDPMNL 123 G+VLSPGFIDVHTHDDTNVIR P+MFPKLSQGVTTVVVGNCGISA+PVTLKG+PPDPMNL Sbjct: 62 GRVLSPGFIDVHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGEPPDPMNL 121 Query: 124 LGQSTAFSYPTFASYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASDAEIVGMREQ 183 LG+ AF YP+FASYV A+ AARP++NVAALVGHTALR+NHMDRLDRAA+++EI GMR Q Sbjct: 122 LGKREAFHYPSFASYVEAVNAARPAINVAALVGHTALRNNHMDRLDRAATESEIAGMRAQ 181 Query: 184 LTEALEHGALGLSTGLAYGNAINAPTGEVLALAEPLAEYGAMYATHLRSEFADILVAMDE 243 L EALEHGALGLSTGLAYGNAINAPT EVLALAEPLAE+G +YATHLRSEFADIL AM+E Sbjct: 182 LREALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILDAMEE 241 Query: 244 AFRIGKHARVPVVISHLKCAGVENWGRSSEVLAALEQAEKHQHVGCDCYPYTASSSTLDL 303 +FRIGKHARVPVVISHLKCAGVENWGRSSEVL ALE+A +HQHVGCDCYPYTASSSTLDL Sbjct: 242 SFRIGKHARVPVVISHLKCAGVENWGRSSEVLHALERAARHQHVGCDCYPYTASSSTLDL 301 Query: 304 KQVTDTYPIQVTWSEPHPEQGGKLLSEIAAGW 335 KQVT Y IQVTWSEPHPEQGGK+L +IAA W Sbjct: 302 KQVTGDYDIQVTWSEPHPEQGGKMLKQIAAEW 333 >gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 498 Score = 271 bits (694), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 147/319 (46%), Positives = 195/319 (61%) Query: 17 DTLIRNVFLVDGSGNDPVVADVAIAGGRIAAIGALGDWQAQQVMDGTGKVLSPGFIDVHT 76 D LI ++DGS AD+ + GRIA IG L QA Q +D +GK+++PGFID HT Sbjct: 8 DLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAPGFIDSHT 67 Query: 77 HDDTNVIRMPDMFPKLSQGVTTVVVGNCGISASPVTLKGDPPDPMNLLGQSTAFSYPTFA 136 HDD V+ PDM K+SQGVTTV+ GNCGIS +P+ P P++LL + + TF+ Sbjct: 68 HDDQAVLSQPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADGTRFETFS 127 Query: 137 SYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASDAEIVGMREQLTEALEHGALGLS 196 SY+ A+ +VNVAA+VGH+ LR MD L+R A EI M L EALE GA+G+S Sbjct: 128 SYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALEAGAIGIS 187 Query: 197 TGLAYGNAINAPTGEVLALAEPLAEYGAMYATHLRSEFADILVAMDEAFRIGKHARVPVV 256 TG Y A +A T EV+ + P+A GA+Y TH+R E + A++E+F IG+ PVV Sbjct: 188 TGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGRELNAPVV 247 Query: 257 ISHLKCAGVENWGRSSEVLAALEQAEKHQHVGCDCYPYTASSSTLDLKQVTDTYPIQVTW 316 ISH K N+G+SS+ LA + QA + Q V DCYPY ASS+ L I ++ Sbjct: 248 ISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLVGRILISS 307 Query: 317 SEPHPEQGGKLLSEIAAGW 335 S+PHPEQ G+ L IAA W Sbjct: 308 SKPHPEQAGRELDAIAAEW 326 >gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 91/311 (29%), Positives = 152/311 (48%), Gaps = 39/311 (12%) Query: 4 FDTSDVS-SSAHSCDTLIRNVFLVDGSGNDPVVADVAIAGGRIAAIGA--LGDWQAQQVM 60 F TS V+ + D +I++ + DG ++P+ ++ IAG RI +G + A +V+ Sbjct: 35 FATSGVAIAQPVQLDLIIQSDRIYDGEHDEPLSGELGIAGDRIVYVGKADRSRFTALRVI 94 Query: 61 DGTGKVLSPGFIDVHTHDDTNVI------RMPDMFPKLSQGVTTVVVGNCGISASPVTLK 114 D G +++PGFID HTH D ++ R D F L+QGVTT V+GN G + V + Sbjct: 95 DARGAIVAPGFIDAHTHADRDMFTDDIKKRQVDYF--LTQGVTTAVIGNDGGGSYEVAQQ 152 Query: 115 GDPPDPMNLLGQSTAFSYPTFASYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASD 174 A++++ G N A VG +R + + D+ Sbjct: 153 ---------------------AAWLSRHGIG---PNAAMYVGFGPIRRSQLGEADQQPDQ 188 Query: 175 AEIVGMREQLTEALEHGALGLSTGLAYGNAINAPTGEVLALAEPLAEYGAMYATHLRSEF 234 ++ M+ + + + GA+G STGL Y A TGEV+ALA+ A+YG +Y +H+R E Sbjct: 189 KQLEAMKALVAKGMCEGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIYDSHIRDEA 248 Query: 235 A---DILVAMDEAFRIGKHARVPVVISHLKCAGVENWGRSSEVLAALEQAEKHQ-HVGCD 290 + + ++ E RI + A + ++H+K G G+S ++ +E A + D Sbjct: 249 SYNVGLAESIREVIRIAREAGIAANVAHIKALGKSVEGQSGAIIRMIEAARAQGLRITAD 308 Query: 291 CYPYTASSSTL 301 YP+ ASS+ Sbjct: 309 QYPWLASSTRF 319 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,082,258 Number of extensions: 44537 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 3 Length of query: 337 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 251 Effective length of database: 1,212,660 Effective search space: 304377660 Effective search space used: 304377660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)