BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0288.p (296 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 356 1e-99 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 84 1e-17 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 82 5e-17 gi|300309779|ref|YP_003773871.1| fructokinase [Herbaspirillum se... 55 7e-09 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 55 7e-09 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 356 bits (913), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 183/238 (76%), Positives = 201/238 (84%) Query: 1 MSKKILAYGEAMVEFNQSIDNPRYYLEGFGGDTSNFCIAAARQGADTAYVSAVGDDHFGK 60 MSK ILAYGEAMVEFNQ ID PR +L+G+GGDTSNF IAAARQGA +AY+SAVGDDHFG+ Sbjct: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQ 60 Query: 61 SLQALWKAERVDDAHVSVVQDAPSGVYFVTHDNAGHHFTYLRSNSAASRYTPAHLPLQAI 120 L ALW+ E VD HV++ +G+YFVTHD AGHHF Y R+ SAAS Y LPL+AI Sbjct: 61 DLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAASAYQAKALPLEAI 120 Query: 121 AEAKVLHLSGISLAISESACDAGLAAMAHARKNGTLTSLDTNLRLRLWPLERARARMAEA 180 A A LHLSGISLAISESAC+AGLAAMAHAR +G TSLDTNLRLRLWPLERARARM EA Sbjct: 121 AAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEA 180 Query: 181 FALCDICLPSWEDVSALTGLEDRDAIVDKLLGYGVKLVAFKLGKDGCYVATSAERRMV 238 F LCDICLPSWEDVS LTGLEDRDAIVD+LL YGVKLVAFKLGK+GCYVATS +RRMV Sbjct: 181 FRLCDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQRRMV 238 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 84.0 bits (206), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 69/235 (29%), Positives = 97/235 (41%), Gaps = 7/235 (2%) Query: 1 MSKKILAYGEAMVEFNQSIDNPRYYLEGF----GGDTSNFCIAAARQGADTAYVSAVGDD 56 M ++ +GEA+ P +E + G +N I AR G + S VG+D Sbjct: 3 MQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 Query: 57 HFGKSLQALWKAERVDDAHVSVVQDAPSGVYFVTHDNAGHH--FTYLRSNSAASRYTPAH 114 FG+ ++ E V+ + V + + + G Y R SAAS + Sbjct: 63 AFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSVDD 122 Query: 115 LPLQAIAEAKVLHLSGISLAISESACDAGLAAMAHARKNGTLTSLDTNLRLRLWPLERAR 174 A+ LH +GI+ A+S + AM R G S D NLR LWP + Sbjct: 123 FDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQEVM 182 Query: 175 ARMAEAFAL-CDICLPSWEDVSALTGLEDRDAIVDKLLGYGVKLVAFKLGKDGCY 228 A+ A A D LP + LTG +D I L GVKLV KLG +G Y Sbjct: 183 AQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAY 237 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 82.4 bits (202), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 72/238 (30%), Positives = 106/238 (44%), Gaps = 24/238 (10%) Query: 12 MVEFNQSIDNPRYYLEGFGGDTSNFCIAAARQGADTAYVSAVGDDHFGKSLQALWKAERV 71 M Q+I + + G GG SN +AAAR GA ++SA+G D FG +LW+ E + Sbjct: 27 MPGLGQTIAGTGFAM-GPGGKGSNQAVAAARVGAPVRFISAIGKDAFGDFALSLWRRETI 85 Query: 72 DDAHVSVVQDAPSGVYFVTHDNAGHHFTYLRSNSAASRYTPAHL--PLQAIAEAKVLHLS 129 HV V+ AP+G F+ ++A + AAS+ + + QAI AKV Sbjct: 86 AP-HVRVIDGAPTGAAFIYVNDASGDNAIIVVPGAASQLSEQDVERERQAIEAAKVF--- 141 Query: 130 GISLAISESACDAGLAAMAHARKNGTLTSLDTNLRLRLWPLERARARMAEAFALCDICLP 189 + E +A LA + AR GT T + A A A+ + LCD P Sbjct: 142 ---VTQLEQPPEAALAGLQIARAAGTTTVFNP---------APALAFPAQVYGLCDFITP 189 Query: 190 SWEDVSALTGLE-----DRDAIVDKLLGYGVKLVAFKLGKDGCYVATSAERRMVPAXC 242 + + + LTG+E D D LL GV LG G + + ++ +PA C Sbjct: 190 NEHEAALLTGIEIHHIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLPALC 247 >gi|300309779|ref|YP_003773871.1| fructokinase [Herbaspirillum seropedicae SmR1] Length = 301 Score = 55.1 bits (131), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 50/163 (30%), Positives = 71/163 (43%), Gaps = 5/163 (3%) Query: 2 SKKILAYGEAMVEFNQSIDNPRYYLEGFGGDTSNFCIAAARQGADTAYVSAVGDDHFGKS 61 S + +GEA+ +F + D + G G N AAAR G T Y +V D FG Sbjct: 4 SAAFVVFGEALTDFIRQPDEQWRAIPG--GACWNVARAAARLGVPTGYAGSVSTDVFGAE 61 Query: 62 LQALWKAERVDDAHVSVVQDAPSGVYFVTHDNAGHHFTYLRSNSAASRYTPAHLPLQAIA 121 L AL +A +D AP + VT +F ++ +SA + P LP + Sbjct: 62 LYALTQAAGLDMRFTQQYAKAPL-LAMVTSTTPPDYF-FIGDDSADLHFDPQRLPAGWLD 119 Query: 122 EAKVLHLSGISLAISESACDAGLAAMAHARKNGTLTSLDTNLR 164 +A+VLH ISL A A AR+N + + D N R Sbjct: 120 DAQVLHFGCISLTREPLASRLLQIAQEAARRNKKI-AFDPNWR 161 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 55.1 bits (131), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 41/143 (28%), Positives = 66/143 (46%), Gaps = 2/143 (1%) Query: 29 FGGDTSNFCIAAARQGADTAYVSAVGDDHFGKSLQALWKAERVDDAHVSV-VQDAPSGVY 87 GG ++N AR G +A ++ +GD+ G+ L+A AE D +HV+V Q + V Sbjct: 52 LGGSSANIAFGCARLGLKSAMLARIGDEANGQFLKAELAAEGCDVSHVTVDPQRLTALVM 111 Query: 88 FVTHDNAGHHFTYLRSNSAASRYTPAHLPLQAIAEAKVLHLSGISLAISESACDAGLAAM 147 D + R N A P + IA +K L ++G + + + L A+ Sbjct: 112 LGLKDKDSFPLIFYRDNCADMALAPEDIDEAFIASSKALLITGTHFSTPQVHRSSSL-AL 170 Query: 148 AHARKNGTLTSLDTNLRLRLWPL 170 +AR++ T LD + R LW L Sbjct: 171 QYARRHNVRTVLDIDYRPVLWGL 193 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.131 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 837,756 Number of extensions: 32628 Number of successful extensions: 106 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 5 Length of query: 296 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 211 Effective length of database: 1,217,395 Effective search space: 256870345 Effective search space used: 256870345 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)