BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0272.p (237 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 191 4e-50 gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 178 4e-46 gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 67 1e-12 gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptid... 55 6e-09 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 191 bits (486), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 106/232 (45%), Positives = 139/232 (59%), Gaps = 19/232 (8%) Query: 19 ACASAPYQGDSYTVRKGDTLMSISRTVGKDHHDIAAWNKLSDANEIYPGDVLRLKPPATA 78 CAS YTV++GDTL SI R + ++ WN+L + +++ G + ++PP Sbjct: 23 GCASTADSDGYYTVKRGDTLYSIGRDFDQSPANLRTWNRLRNPDDLEVGQRILVRPPVGV 82 Query: 79 AVAAPVSRA-----------------KPAAAVHERKAEPKTEREAAAPAATEAERLDWMW 121 ++ KPA + K K + + A PA EA ++DW W Sbjct: 83 VARTGAGQSPRPAASSSPSASGSGSRKPAPSRPTAKPAAKPQDDDA-PAGAEA-KVDWSW 140 Query: 122 PAQGKLTATTDRNLKGIDITGTPGQPVWAAEAGKVTYAGRGIRGYGNMIIVKHSRTLLSV 181 P GK + D KGIDI G+ GQ V AA AGKVTYAG GIRGYGNM+I+KH+ LLSV Sbjct: 141 PTAGKSAPSADSYKKGIDIIGSEGQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSV 200 Query: 182 YAHNKSIMVKEGQTVNRGQQIAEMGDTDSRTVKLYFEIRSNGKALNPVPLLP 233 YAHNK+I+VKEGQTV RGQQIA MG++D+ VKLYFEIR NGK +N + +LP Sbjct: 201 YAHNKTILVKEGQTVTRGQQIATMGNSDTNKVKLYFEIRRNGKPINVMAVLP 252 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 178 bits (451), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 118/333 (35%), Positives = 155/333 (46%), Gaps = 99/333 (29%) Query: 2 LRYISIVAAAVVVAALTACASAPYQGDS-----------------------------YTV 32 ++ I + VV+ L AC S P Q + YTV Sbjct: 1 MKKIRLAVLGSVVSLLAACGSTPNQQPAPVVERTSSTGKVVDAATAPAAPAPSGRGYYTV 60 Query: 33 RKGDTLMSISRTVGKDHHDIAAWNKLSDANEIYPGDVLRLKP------------------ 74 +KGDTL I+ G++ DI AWN L++ N+I LR+ P Sbjct: 61 KKGDTLYRIALEAGQNWRDIVAWNNLTNPNDIKVDQTLRVAPPDRAPGTAQTAAIAASPA 120 Query: 75 ----------PATAAVAAPVS--------RAKPAAAVHERKAEPKTEREAAAPAAT---- 112 P T+A AAPV R AE + +APAA+ Sbjct: 121 AGLEVKPLGAPGTSAPAAPVPVGSNKTGPRGDKRPYSESALAELEKPDAPSAPAASGTSV 180 Query: 113 -----------------------------EAERLDWMWPAQGKLTATTDRNLKGIDITGT 143 + E + WMWPA+GK+ T + KG+DI G Sbjct: 181 ASAAPSAPAKAEQPHAADKPAAAASQAAADDEGVSWMWPAEGKVVGTFEGGKKGVDIAGK 240 Query: 144 PGQPVWAAEAGKVTYAGRGIRGYGNMIIVKHSRTLLSVYAHNKSIMVKEGQTVNRGQQIA 203 GQ V AA AGKV YAG GIRGYGN++I+KH+ LLS YAHN++I+VKEGQ+V +GQ+IA Sbjct: 241 AGQAVLAAGAGKVMYAGSGIRGYGNLVIIKHTSNLLSAYAHNQTILVKEGQSVTKGQKIA 300 Query: 204 EMGDTDSRTVKLYFEIRSNGKALNPVPLLPKPR 236 EMG +DS TVKL+FEIR GK ++P LP PR Sbjct: 301 EMGKSDSDTVKLHFEIRQQGKPVDPSKYLP-PR 332 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 67.0 bits (162), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 34/94 (36%), Positives = 55/94 (58%), Gaps = 2/94 (2%) Query: 136 KGIDITGTPGQPVWAAEAGKVTYAGRGIRGYGNMIIVKHSRTLLSVYAHNKSIMVKEGQT 195 +G+ I G V A AG+V +A +RG+GN+IIV H +++Y +N+S++ + G Sbjct: 371 RGLFIRAHEGAEVRAVAAGRVVFAD-WLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDL 429 Query: 196 VNRGQQIAEMGDT-DSRTVKLYFEIRSNGKALNP 228 V G IA G++ + LYFE+R G+A +P Sbjct: 430 VKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDP 463 >gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 55.1 bits (131), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 26/68 (38%), Positives = 39/68 (57%), Gaps = 1/68 (1%) Query: 166 YGNMIIVKHSRTLLSVYAHNKSIMVKEGQTVNRGQQIAEMGDTDSRT-VKLYFEIRSNGK 224 +GNM+ + H +++ YAH + VK G V RGQ +A++G T T L+FE+R G Sbjct: 238 FGNMLEIDHGNNIITRYAHASRLFVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGI 297 Query: 225 ALNPVPLL 232 A +P L Sbjct: 298 AQDPRKFL 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 582,132 Number of extensions: 19830 Number of successful extensions: 65 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 64 Number of HSP's successfully gapped: 5 Length of query: 237 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 155 Effective length of database: 1,231,600 Effective search space: 190898000 Effective search space used: 190898000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)