BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0270.p (439 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313097|ref|YP_003777189.1| enzyme with a TIM-barrel fold p... 232 4e-62 gi|300309842|ref|YP_003773934.1| enzyme with a TIM-barrel fold p... 81 2e-16 >gi|300313097|ref|YP_003777189.1| enzyme with a TIM-barrel fold protein [Herbaspirillum seropedicae SmR1] Length = 233 Score = 232 bits (592), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 124/193 (64%), Positives = 143/193 (74%) Query: 205 AAAQAAGRNPQDILLLAVSKFFDAASVLDAVHAGQTAFGENYVQEALDKIAALKLTAPEA 264 AAA+ AGR + LLAVSK F +VL+AV AGQ AFGENY+QEAL+KIAAL L AP+ Sbjct: 17 AAAERAGRPAGAVQLLAVSKTFGPQAVLEAVRAGQRAFGENYLQEALEKIAALPLLAPDV 76 Query: 265 QVAWHFIGPIQSNKTRQIAENFDWVHSVDREKIARRLAEQRPPGLPPLNICLQVNISDED 324 + WHFIGPIQSNKTR IAE+FDWVHSVDR KIA+RL+EQRP L LN+CLQVNIS ED Sbjct: 77 PLQWHFIGPIQSNKTRPIAEHFDWVHSVDRLKIAQRLSEQRPAALGALNVCLQVNISGED 136 Query: 325 SKSGLSPDELLPVARAVAALPGLRLRGLMAIPAPTEVEEEQRAAFRRLRELAALLTDSGI 384 SKSGL P EL VA VA LPGL+LRGLMAIPAP+E +QRAAF +R L A L G+ Sbjct: 137 SKSGLDPAELPAVAAQVAQLPGLKLRGLMAIPAPSEDVTQQRAAFAAVRSLLAQLQAQGL 196 Query: 385 ACDNNDSPYNIDV 397 A D + D+ Sbjct: 197 ALDTLSMGMSADL 209 Score = 102 bits (254), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 54/81 (66%), Positives = 63/81 (77%) Query: 10 AAAQAAGRNPQDILLLAVSKFFDAASVLDAVHAGQTAFGENYVQEALDKIAALKLTAPEA 69 AAA+ AGR + LLAVSK F +VL+AV AGQ AFGENY+QEAL+KIAAL L AP+ Sbjct: 17 AAAERAGRPAGAVQLLAVSKTFGPQAVLEAVRAGQRAFGENYLQEALEKIAALPLLAPDV 76 Query: 70 QVAWHFIGPIQSNKTRQIAEN 90 + WHFIGPIQSNKTR IAE+ Sbjct: 77 PLQWHFIGPIQSNKTRPIAEH 97 >gi|300309842|ref|YP_003773934.1| enzyme with a TIM-barrel fold protein [Herbaspirillum seropedicae SmR1] Length = 270 Score = 81.3 bits (199), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 56/151 (37%), Positives = 80/151 (52%), Gaps = 3/151 (1%) Query: 205 AAAQAAGRNPQDILLLAVSKFFDAASVLDAVHAGQTAFGENYVQEALDKIAALKLTAPEA 264 AA + AGR + LL VSK +S+ A AG GEN QEA K A T + Sbjct: 44 AACERAGRPASSVRLLPVSKTKPESSIRLAYAAGCCLLGENKPQEACHKWEA---TQDLS 100 Query: 265 QVAWHFIGPIQSNKTRQIAENFDWVHSVDREKIARRLAEQRPPGLPPLNICLQVNISDED 324 + W IG +Q+NK + +A ++D ++A+ L + L++ +QVN S E Sbjct: 101 GLQWSVIGHLQTNKAKLVARFAAEFQALDSLRVAQALERRLQQEGRGLDVFVQVNTSGEA 160 Query: 325 SKSGLSPDELLPVARAVAALPGLRLRGLMAI 355 SK GL+P+E+ P R + A P LRLRGLM + Sbjct: 161 SKYGLAPEEVAPFLRQLHAYPALRLRGLMTL 191 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,092,121 Number of extensions: 39181 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 3 Length of query: 439 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 351 Effective length of database: 1,203,190 Effective search space: 422319690 Effective search space used: 422319690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)