BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0264.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313101|ref|YP_003777193.1| sigma54-dependent transcription... 162 2e-41 >gi|300313101|ref|YP_003777193.1| sigma54-dependent transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 162 bits (411), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 87/130 (66%), Positives = 95/130 (73%), Gaps = 5/130 (3%) Query: 167 NLRQMHAVIRYCCAVMNGDRIEAGDLPEELMETFAPSTSHAIHYEGPAISPASNVRSIVP 226 NLRQMHAVIRY CAVMNG RIE DLPEELM+ F P+T +H I+ + +R Sbjct: 309 NLRQMHAVIRYSCAVMNGGRIEVRDLPEELMQ-FQPATLAEVH----PITRTAQIRPFTS 363 Query: 227 QAGAADILPRPSGGPLGDERTRVIDALVGSRWNISAASRQLGMCRASLYRKLRQLDIPHV 286 +A R SG P DERTRVIDAL GSRWNISAASRQLGMCRASLYRKLRQLDIPHV Sbjct: 364 RAEGEAGRERSSGDPYRDERTRVIDALNGSRWNISAASRQLGMCRASLYRKLRQLDIPHV 423 Query: 287 RDQAMPTHFA 296 RDQAMP + A Sbjct: 424 RDQAMPAYPA 433 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,053,964 Number of extensions: 47286 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 1 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 130 (54.7 bits)