BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0263.p (127 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 152 6e-39 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 74 3e-15 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 68 2e-13 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 62 2e-11 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 61 2e-11 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 152 bits (385), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 68/103 (66%), Positives = 89/103 (86%) Query: 1 VVAADVSKTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGPVEASEESWNRVIAINQTSV 60 V+AADVS+ +++A+ +AA+DHFGR+DVLHNNVGIA+ GGPVE SEESWNR++A+NQTS+ Sbjct: 65 VLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVNQTSL 124 Query: 61 FMTCKHVLPVMEKQKKGAIVNISSLAAIRWVIISPVYLXALRA 103 F+T KHVLP+MEKQ+KGAIVNISSLAA+RW+ + A +A Sbjct: 125 FLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKA 167 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 74.3 bits (181), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 34/87 (39%), Positives = 55/87 (63%), Gaps = 1/87 (1%) Query: 4 ADVSKTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGP-VEASEESWNRVIAINQTSVFM 62 ADVSK D++ ++ AL+HFG++D++ NN G P +E E+ ++RV A+N S+F+ Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 Query: 63 TCKHVLPVMEKQKKGAIVNISSLAAIR 89 + K +P + GA +NI+S A IR Sbjct: 121 SAKTFVPYFRQVGGGAFINIASTAGIR 147 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 68.2 bits (165), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 35/88 (39%), Positives = 47/88 (53%) Query: 2 VAADVSKTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGPVEASEESWNRVIAINQTSVF 61 V DV++ D+EA+V HFGRIDVL NN GI + E W+R+I +N V Sbjct: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 120 Query: 62 MTCKHVLPVMEKQKKGAIVNISSLAAIR 89 LP Q G I+N+SS+A I+ Sbjct: 121 YGVAAALPRFAAQSSGHIINVSSVAGIK 148 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 61.6 bits (148), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 34/85 (40%), Positives = 51/85 (60%), Gaps = 2/85 (2%) Query: 5 DVSKTPDIEAMVQAALDHF-GRIDVLHNNVGIAETGGPVE-ASEESWNRVIAINQTSVFM 62 DVS +++AM + GR+DVL NN GIA PVE + W++ +A+N T F+ Sbjct: 62 DVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPFL 121 Query: 63 TCKHVLPVMEKQKKGAIVNISSLAA 87 + +P+++K GAIVNISS+A Sbjct: 122 CTRRAVPLLKKNGGGAIVNISSVAG 146 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 61.2 bits (147), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 31/84 (36%), Positives = 49/84 (58%), Gaps = 1/84 (1%) Query: 5 DVSKTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGPVEASEESWNRVIAINQTSVFMTC 64 DVS + +V A++ FG++DVL ++ G+ G E S E W+RV A N VF Sbjct: 69 DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGA 128 Query: 65 KHVLPVMEKQKKGAIVNISSLAAI 88 + LP +EK +G IVN++S++ + Sbjct: 129 REALPHLEK-TRGCIVNVASVSGL 151 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.130 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 324,003 Number of extensions: 10488 Number of successful extensions: 79 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 12 Length of query: 127 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 52 Effective length of database: 1,264,745 Effective search space: 65766740 Effective search space used: 65766740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)