BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0259.p (110 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 164 1e-42 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 65 9e-13 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 62 9e-12 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 61 2e-11 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 57 2e-10 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 164 bits (416), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 74/91 (81%), Positives = 84/91 (92%) Query: 19 QQRAAWYAPLGIRANCVLPGLMDTPMIREPLKASYGGDIEEMRRKRHAQCPMGHMGDAWD 78 + A YAP+GIRANCVLPG+MDTPMIREPLKASYGGDIE+MR KR+AQCPMG MGDAWD Sbjct: 174 KNVAMQYAPMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWD 233 Query: 79 VAHAALFLASDAARYVTGVDLIVDGGLSLKC 109 VAHA+LFLASD ARY+TG+D+IVDGGLSL+C Sbjct: 234 VAHASLFLASDHARYITGLDMIVDGGLSLRC 264 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 65.5 bits (158), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 38/113 (33%), Positives = 58/113 (51%), Gaps = 15/113 (13%) Query: 1 VARHRRQDVIGGRHHEAHQQ--------RAAWYAPLGIRANCVLPGLMDTPMIREPLKAS 52 V+ R ++GG H+ A + A + P GIR NC+ PGL+ T + ++ L A Sbjct: 141 VSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRVNCITPGLISTDINKDILSAE 200 Query: 53 YGGDIEEMRRKRHAQCPMGHMGDAWDVAHAALFLASDAARYVTGVDLIVDGGL 105 +I + P+G +G DV +FLASD ++Y TGV L V+GG+ Sbjct: 201 KKAEIAQ-------TIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTLDVNGGM 246 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 62.0 bits (149), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 36/113 (31%), Positives = 57/113 (50%), Gaps = 15/113 (13%) Query: 1 VARHRRQDVIGGRHHEAHQQ--------RAAWYAPLGIRANCVLPGLMDTPMIREPLKAS 52 V+ R ++GG H+ A + A + P GIR N + PGL+ T +I+ L Sbjct: 141 VSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIRVNSITPGLIGTDIIKGKLT-- 198 Query: 53 YGGDIEEMRRKRHAQCPMGHMGDAWDVAHAALFLASDAARYVTGVDLIVDGGL 105 EE + + P+ +G A D+ A +FL SD ++Y TG+ L V+GG+ Sbjct: 199 -----EEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITLDVNGGM 246 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 61.2 bits (147), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 37/94 (39%), Positives = 49/94 (52%), Gaps = 6/94 (6%) Query: 17 AHQQRAAWYAPLGIRANCVLPGLMDTPMIREPLKASYGGDIEEMRRKRHAQCPMGHMGDA 76 + AA P IR NCV P + T ++ E + D E R+K A PMG Sbjct: 165 TSKSMAAELGPDKIRVNCVNPVISATGLLSEFMGVP---DTPENRKKFVATIPMGRFSTP 221 Query: 77 WDVAHAALFLASDAARYVTGVDLIVDGGLSLKCV 110 D+A+A L+L SD A +VTGV + VDGG +CV Sbjct: 222 EDIANACLYLGSDEAEFVTGVCIEVDGG---RCV 252 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 57.4 bits (137), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 32/91 (35%), Positives = 50/91 (54%), Gaps = 3/91 (3%) Query: 19 QQRAAWYAPLGIRANCVLPGLMDTPMIREPLKA---SYGGDIEEMRRKRHAQCPMGHMGD 75 + AA + GIR N + PG +++P + + + G EE+R + PMG +G Sbjct: 163 KSVAADFVAQGIRCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGK 222 Query: 76 AWDVAHAALFLASDAARYVTGVDLIVDGGLS 106 A +VA AL+LASD + + TG ++DGG S Sbjct: 223 AEEVAALALYLASDESNFTTGSIHMIDGGWS 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 368,762 Number of extensions: 13462 Number of successful extensions: 91 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 8 Length of query: 110 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 37 Effective length of database: 1,274,215 Effective search space: 47145955 Effective search space used: 47145955 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 124 (52.4 bits)