BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0255.p (572 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 1135 0.0 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 169 5e-43 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 137 3e-33 gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 92 8e-20 gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspi... 57 3e-09 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 1135 bits (2936), Expect = 0.0, Method: Compositional matrix adjust. Identities = 535/572 (93%), Positives = 558/572 (97%) Query: 1 MSAENLTGAEILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA 60 MSAE LTGA+ILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA Sbjct: 1 MSAEQLTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA 60 Query: 61 DAYSRSSEKVGVCIVTSGPGVTNAVTGLATAYMDSIPMIVISGQVPSTAIGQDAFQECDT 120 DAYSRSS+KVGV IVTSGPGVTNAVTGLATAYMDSIPM+VISGQVPSTAIGQDAFQECDT Sbjct: 61 DAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDT 120 Query: 121 VGITRPCVKHNFLVKDVKDLAETMKKAFYIATTGRPGPVLVDIPKDISMHTCAYEYPKTV 180 VGITRPCVKHNFLVKDVKDLAET+KKAFYIATTGRPGPVLVDIPKDI+MHTCAY YPK V Sbjct: 121 VGITRPCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAV 180 Query: 181 EMRSYKPVDKGHSGQIRKALQLLLTAERPMIYTGGGVILANAAPELNKLVDRLGFPCTNT 240 EMRSYKPVDKGHSGQIRKALQLLL+AERPMIYTGGG+ILANAAPELNKLVDRLG+PCTNT Sbjct: 181 EMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNT 240 Query: 241 LMGLGAYRSTSDKFVGMPGMHGTYEANMAMQHSDVMIAIGARFDDRVIGNPKHFASNARK 300 LMGLGAYRS+SDKFVGMPGMHGTYEANMAMQH DV+IAIGARFDDRVIGNPKHFAS+ARK Sbjct: 241 LMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARK 300 Query: 301 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLTQLDAAETKPNAPALANWWKQINKWRE 360 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELL+QLDAAET+ N AL WWKQIN+WR+ Sbjct: 301 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRK 360 Query: 361 RDCLKFATSDEFIKPQAVIKNVWDITKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 RDCLKFATS+EFIKPQ+V++ VW++TKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG Sbjct: 361 RDCLKFATSEEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 421 LGTMGVGLPYAMGVQKANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 LGTMGVGLPYAMGVQ ANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 481 MVRQWQQIDYGSRYSESYMDSLPDFTKLAESYGHVGMKIEKPGDVDGALKEAFGMKDKLV 540 MVRQWQQIDYGSRYSESYMDSLPDF KLAESYGHVGMKIEKPGDVDGAL+EAF MKD+LV Sbjct: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 Query: 541 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL 572 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL Sbjct: 541 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL 572 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 169 bits (428), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 146/557 (26%), Positives = 237/557 (42%), Gaps = 24/557 (4%) Query: 7 TGAEILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRS 66 T A+IL+ L E GV +FG G A+ + DAI + + + I VRHE+ A AA ++ Sbjct: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKL 63 Query: 67 SEKVGVCIVTSGPGVTNAVTGLATAYMDSIPMIVISGQVPSTAIGQDAFQECDTVGITRP 126 + ++ VC T+GPG + V GL A D P++ ISG VP+ G D QE + R Sbjct: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRD 123 Query: 127 CVKHNFLVKDVKDLAETMKKAFYIATTGRPGPVLVDIPKDISMHTCAYEYPKTVEMRSYK 186 ++ ++ +A A R G ++IP D+ + P +R Sbjct: 124 VAAYSQIITSPDQAPAVFHQAIAQAYAQR-GVAHLNIPADVIGAKTSGSVPSLHTLRERT 182 Query: 187 PVDKGHSGQIRKALQLLLTAERPMIYTGGGVILANAAPELNKLVDRLGFPCTNTLMGLGA 246 P+ + I+ A L+ A I+ G G A E+ L +L P +T G Sbjct: 183 PLSP-PAADIQAAADLINAAGSVAIFAGNGC--RGAIDEVLALASKLQAPVMHTFRGKDL 239 Query: 247 YRSTSDKFVGMPGMHGTYEANMAMQHSDVMIAIGARFDDRVIGNPKHFASNARKIIHIDI 306 ++G G+ G A+ ++VM+ +G+ + F K+I ID Sbjct: 240 ASYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPY------SEFLPTKSKVIQIDE 293 Query: 307 DPSSISKRVKVDIPIVGNVKDVLQELLTQLDAAETKPNAPALANWWKQINKWRERDCLKF 366 + +R V + I G+V +Q+LL ++ E K + L +Q N RER K Sbjct: 294 RGFVLGRRAPVTLGITGSVAPAVQQLLERV---EGKTDGAFL----QQANAHRERWNAKL 346 Query: 367 ATSDEF------IKPQAVIKNVWDITKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 ++ E IKPQ+V + + D+ DA D G +W + +R + S Sbjct: 347 DSAAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFN 406 Query: 421 LGTMGVGLPYAMGVQKANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 +G L A G+Q + + V G+ M + E T ++ L K+++ NNR G Sbjct: 407 NAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWG 466 Query: 481 MVRQWQQIDYGSRYSESYMDSLPDFTKLAESYGHVGMKIEKPGDVDGALKEAFGMKDKLV 540 +V + + G E PDF A + G G + P +++G L++ V Sbjct: 467 LV-HLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAV 525 Query: 541 FMNFITDQTENVWPMVK 557 FI V P +K Sbjct: 526 LDVFIDPDELPVMPHIK 542 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 137 bits (345), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 140/557 (25%), Positives = 244/557 (43%), Gaps = 70/557 (12%) Query: 20 GVEHVFGYPG----GAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSEKVGVCIV 75 GV +FG+ G LY Y A + +EQA H+A AY+++ + + V Sbjct: 40 GVFSIFGHGNVAGMGEALYQYRATLPTYR-----AHNEQAMAHSAIAYAKAHLRQRMMAV 94 Query: 76 TS--GPGVTNAVTGLATAYMDSIPMIVISGQV-----PSTAIGQDAFQECDTVGIT---- 124 T+ GPG TN +T A A+++ +P++++ G V P + Q ++ + G++ Sbjct: 95 TTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFVSRRPDPVLQQ--LEDFNDPGLSVNDA 152 Query: 125 -RPCVKHNFLVKDVKDLAETMKKAFYIAT-TGRPGPVLVDIPKDISMHTCAYEYP----- 177 RP + + + L + +A + T + GPV + +P+D+ T AY+YP Sbjct: 153 FRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGPVTLALPQDV--QTMAYDYPLDFFS 210 Query: 178 -KTVEMRSYKPVDKGHSGQIRKALQLLLTAERPMIYTGGGVILANAAPELNKLVDRLGFP 236 + + R+ P + ++ A+ LL A +P++ GGGV+ NA L + ++ G P Sbjct: 211 PRVIVPRAQAPAAQ----ELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVP 266 Query: 237 CTNTLMGLGAYRSTSDKFVGMPGMHGTYEANMAMQHSDVMIAIGARFDDRVIGNPKHFAS 296 T G A + +G G+ G+ AN Q +DV+IA+G R D G+ FA Sbjct: 267 VAETQAGKSALPWSHALQMGAIGVTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQ 326 Query: 297 NARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLTQLDAAETKPNAPALANWWKQIN 356 ++++++++ K + + + L+ L L+ + P A A+ Q Sbjct: 327 A--RLLNLNVNAMDAHKWRGLALQADAGLG--LEALSHALEGWRSAPEWHARAHTLAQ-- 380 Query: 357 KWRERDCLKFATSD---------EFIKPQAVIKNVWDITKGDAFI----TSDVGQHQMWA 403 WRER +D E I A+ ++V D D + T H++W Sbjct: 381 DWRERVAQITGQTDTGGRLPYDGEVIG--AIQRSVADSPSQDIVVCAAGTLPAELHKLWR 438 Query: 404 AQYYGFDKPRRWINSGGLGTMGVGLPYAMGVQKANPDATVACITGEASIQMCIQELATCK 463 A +P + G MG + +GV+ A P V I G+ S M ELAT Sbjct: 439 A-----GRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATSV 493 Query: 464 QYHLTPKIILLNNRFLGMVRQWQQIDYGSRYSESYMDSLP--------DFTKLAESYGHV 515 +++L+NR G + + QQ G+ ++ D L DF A S G + Sbjct: 494 MLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGAL 553 Query: 516 GMKIEKPGDVDGALKEA 532 G ++ +++ AL+ A Sbjct: 554 GENVKTITELEAALQRA 570 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 92.4 bits (228), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 130/534 (24%), Positives = 212/534 (39%), Gaps = 57/534 (10%) Query: 20 GVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSEKVGVCIVTSGP 79 G+ VF PG L ++ F +D F+++L E V AD ++++S + + S Sbjct: 23 GMTSVFANPGSTELPMFRD-FPED-FRYVLGLQEAVVVGMADGFAQASGNAALVNLHSAA 80 Query: 80 GVTNAVTGLATAYMDSIPMIVISGQVPSTAIGQDAF-QECDTVGITRPCVKHNFLVKDVK 138 GV NA+ + TA+ + P+I+ +GQ + + D F + +P +K + Sbjct: 81 GVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPYLKWSIEPARAA 140 Query: 139 DLAETMKKAFYIATTGRPGPVLVDIPKDISMHTCAYEYPKTVEMRSYKPVDKGHSGQIRK 198 D+ + +A+++A GPV V IP D P V R +P + Sbjct: 141 DVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPTQVTSRC-RPEPAAIARLAAA 199 Query: 199 ALQLLLTAERPMIYTGGGVILANA-------APELNKLV------DRLGFPCTNTLMGLG 245 Q ++RP I G + A A A L V R FP + L G Sbjct: 200 LEQ----SQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSSRCSFPEDHPLFA-G 254 Query: 246 AYRSTSDKFVGMPGMHGTYEANMAMQHSDVMIAIGA-RFDDRVIGNPKHFASNARKIIHI 304 + +K V A+Q D++ A+GA F V G+ H A I Sbjct: 255 FLPAMREKIV------------QALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQLI 302 Query: 305 DIDPSSISKRVKVDIPIVGNVKDVLQELLTQLDAAETKPNAPALANWWKQINKWRERDCL 364 D DP + S IVGN++ L +LL Q+ P R R Sbjct: 303 D-DPVTASW-TPTGQAIVGNIQLGLDDLLAQIR------RQPPTQRAQPPARPARARVEA 354 Query: 365 KFATSDEFIKPQAVIKNVWDITK-GDAFITSDVGQHQMWAAQYYGFDKPRRW--INSGGL 421 S + ++ + D+ + GD + G + A + F +P + + SGGL Sbjct: 355 SAPLSMAY-----ALQTLADVREAGDIVVEEAPGSRPVMQA-HLPFTRPGSFYTMASGGL 408 Query: 422 GTMGVGLPYAMGVQKANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGM 481 G G+P A+GV A P + V + G+ S Q + + Q L ++LNN Sbjct: 409 ---GYGMPAAVGVALARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAA 465 Query: 482 VRQWQQIDYGSRYSESYMDSLP--DFTKLAESYGHVGMKIEKPGDVDGALKEAF 533 ++ + + + LP DF LA S G G+++ + + AL+EA Sbjct: 466 LQDFAPVFGFGPEDPVHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREAL 519 >gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 57.4 bits (137), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 120/537 (22%), Positives = 196/537 (36%), Gaps = 71/537 (13%) Query: 7 TGAEILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRS 66 TGA L L ++GV+ F PG L + + K + ++ E AAD Y R Sbjct: 37 TGAHALWAGLLDQGVDTCFANPGTTELDLVRGLESFPKLRCVVALQENVCTGAADGYGRM 96 Query: 67 SEKVGVCIVTSGPGVTNAVTGLATAYMDSIPMIVISGQVPSTAIGQDAFQECDTVGITRP 126 + ++ GPG N + L A P++ I G S + DA D + P Sbjct: 97 LGRPASTLLHLGPGFANGIANLHNARRARTPIVNIIGDHTSWHLPYDAPLSSDIESLAHP 156 Query: 127 CVKHNFLVKDVKDLAETMKKAFYIAT-TGRPGPVLVDIPKDISMHTCAYEYPKTVEMRSY 185 + D+A ++ +T G G L+ P D A P + Sbjct: 157 VSGWVHRITGTADIAAAAAESVRQSTAAGGQGSTLI-FPADFQYADMAEASPAAATEK-- 213 Query: 186 KPVDKGHSGQIR--KALQLLLTAERPMIYTGGGVILANAAPELNKLVDRLGFPCTNTLMG 243 D G + R +AL L A+R + GGG + + V R+ +G Sbjct: 214 ---DTGQADDYRADQALARLQGAQRVVFLLGGGGEESGLGARAQRAVARIA-----AHIG 265 Query: 244 LGAYRST----SDKFVGMPGM----HGTYEANMAMQHSDVMIAIGA-----RFDDRVIGN 290 Y T +++ G+P + A + +D+++ +GA F +G+ Sbjct: 266 CSVYAETFPARAERGQGLPSFDRLPYFPEPARKVLDDADLVVLVGALPPITYFG--YVGH 323 Query: 291 PKHFASNARKIIHIDIDPSSISKRVKVDIPIVGNVKDVL-QELLTQLDAAETKPNAPALA 349 P + R ++ I + + RV+ +G V Q L L E P Sbjct: 324 PSALVAPER-LLTISLPGRPAAGRVEQLADALGAPAYVTPQHALPPLPEGELTP------ 376 Query: 350 NWWKQINKWRERDCLKFATSDEFIKPQAVIKNVWDITKGDAF-ITSDVGQHQMWAAQYYG 408 ++I R+ P+ I +V T G F SD Sbjct: 377 ---QKIAAVLAREL-----------PERAIVSVEGGTCGYPFYAVSDAA----------- 411 Query: 409 FDKPRRWINSGGLGTMGVGLPYAMGVQKANPDATVACITGEASIQMCIQELATCKQYHLT 468 +P + + G G +G GLP A+G A PD TV + + S Q IQ L + + Sbjct: 412 --RPHTVLTNTG-GAIGQGLPVALGAAVACPDRTVVGLLSDGSTQYTIQTLWSLAHENAR 468 Query: 469 PKIILLNNRFLGMVRQWQQID---YGSRYSESYMDSLP--DFTKLAESYGHVGMKIE 520 +++ N ++R + D G R ++ P D+ LA+SYG + E Sbjct: 469 VVVLIAANHQYAILRNELRRDGAPLGERAAQMTALDHPRIDWVGLAQSYGVPASRAE 525 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,794,944 Number of extensions: 75380 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 5 Length of query: 572 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 482 Effective length of database: 1,193,720 Effective search space: 575373040 Effective search space used: 575373040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)