BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0253.p (136 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311062|ref|YP_003775154.1| hypothetical protein Hsero_1733... 160 4e-41 >gi|300311062|ref|YP_003775154.1| hypothetical protein Hsero_1733 [Herbaspirillum seropedicae SmR1] Length = 136 Score = 160 bits (405), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 94/137 (68%), Positives = 111/137 (81%), Gaps = 6/137 (4%) Query: 1 MNNKELQFAYRVKRALDANLEHLPESTTQSLAEARRVALARKKTTAPVRVAASQFALAGA 60 MNNKE+QFAY+VK ALDANL++L + T QSLA ARRVALARKK T P+ V Q LAGA Sbjct: 1 MNNKEMQFAYKVKLALDANLDNLSDDTLQSLAAARRVALARKKQT-PMTVRVEQPVLAGA 59 Query: 61 MG---GNFTPSSSFSWLGRLGVAIPLLAGVMLFAGLYEHEHQQRISALAEIDAGVLSDEL 117 G G +P +S WLGRLGVA PLLAG++LFAGLY+HE+ +R+S LA+IDA VLSDEL Sbjct: 60 FGSAGGMLSPKAS--WLGRLGVAAPLLAGIILFAGLYQHENAKRVSYLADIDAAVLSDEL 117 Query: 118 PLSAYLDHGFNAYLAQQ 134 PLSAYLDHGFNAYLA++ Sbjct: 118 PLSAYLDHGFNAYLAEK 134 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 440,238 Number of extensions: 18370 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 69 Number of HSP's successfully gapped: 1 Length of query: 136 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 60 Effective length of database: 1,260,010 Effective search space: 75600600 Effective search space used: 75600600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)