BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0248.p (107 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311058|ref|YP_003775150.1| hypothetical protein Hsero_1729... 89 1e-19 >gi|300311058|ref|YP_003775150.1| hypothetical protein Hsero_1729 [Herbaspirillum seropedicae SmR1] Length = 115 Score = 88.6 bits (218), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 55/114 (48%), Positives = 78/114 (68%), Gaps = 14/114 (12%) Query: 2 KLIKVI-GAALAIGAVGLAISACQKKEETPGPAETMGKQIDSAVKNAGDQAAVVADKAQE 60 K+ K++ G +++ A+ L ++ACQKKEE PGPAE +GK+ID AV++AG + V +A++ Sbjct: 3 KMSKLLAGVVMSVAAISL-LAACQKKEE-PGPAEALGKKIDGAVQDAGKKLDEVTGQAKD 60 Query: 61 KAAEAGQKLDKAVD-----------DMKEGAKDLTQKAGEKMEAAGQKIQDTAK 103 ++ ++G KLD A+D KEGAKDL QK GEKME AGQKIQD +K Sbjct: 61 QSEQSGSKLDNALDKAKADAKEAYEKTKEGAKDLAQKTGEKMEEAGQKIQDASK 114 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.304 0.121 0.308 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 174,106 Number of extensions: 3267 Number of successful extensions: 30 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 28 Number of HSP's successfully gapped: 1 Length of query: 107 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 35 Effective length of database: 1,278,950 Effective search space: 44763250 Effective search space used: 44763250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (21.9 bits) S2: 124 (52.4 bits)