BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0234.p (338 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 398 e-112 gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogen... 159 2e-40 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 146 2e-36 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 94 1e-20 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 79 4e-16 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 398 bits (1023), Expect = e-112, Method: Compositional matrix adjust. Identities = 192/338 (56%), Positives = 243/338 (71%), Gaps = 1/338 (0%) Query: 1 MLAAVVEQFGGPLVIKEIPLPSPGAGQILVKTEACGVCHTDLHAAHGDWPVKPSLPFVPG 60 M AAVV +FG PL I+++P+P+P GQILVK EA GVCHTDLHAAHGDWPVKP+ PF+PG Sbjct: 5 MKAAVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFIPG 64 Query: 61 HEGIGVVVAIGAGVSEVKMGDRVGVPWLYSACGHCEHCLAAWETVCAHAEFGGYTKNGGF 120 HEG G V A+GAGV VK GDRVGVPWL++ACG C C WET+CA + GY+ NG F Sbjct: 65 HEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSVNGSF 124 Query: 121 AEYILADPRYVAHIPAGLEAAAAAPIICAGVTSYKGIKECQVQAGDWLAVSGIGGLGHLA 180 AEY LADP++V H+P L+ AAP++CAGVT YKG+KE +V+ G+W+ +SGIGGLGH+A Sbjct: 125 AEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGLGHMA 184 Query: 181 VQYGKAMGLRVCAVDIDDGKLAHAQRLGADAVVNARVGDPIARLREVTDGGAHGVLITAP 240 VQY KAMG+ V A DI + KLA A++LGAD V+ R + +A ++ + GGAHG L+TA Sbjct: 185 VQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRII-GGAHGALVTAV 243 Query: 241 SLEAFHQGVAMTRKRGTCVLVGLPPGEFPTPLFDVVANCITIRGSFVGTRKDMAEALAFA 300 S +A Q R RGT LVGLPPG+ P+F+ V IT+RGS VGTR+D+ E+L FA Sbjct: 244 SPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQDLEESLVFA 303 Query: 301 AAGKVRADFELQPLSAINNVFERLQRGDVPSRVVLDFS 338 A GKV A F L IN +F R++ G + R+VLD Sbjct: 304 AEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLDLK 341 >gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 159 bits (403), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 119/336 (35%), Positives = 175/336 (52%), Gaps = 17/336 (5%) Query: 12 PLVIKEIPLPSPGAGQILVKTEACGVCHTDLHAAHGDWPVKPSLPFVPGHEGIGVVVAIG 71 PL I + GA + + CG+CH+DLH +W P VPGHE +G V A+G Sbjct: 14 PLEHLHITRRATGAHDVQIDIAYCGICHSDLHQIRSEW-AGTQYPCVPGHEIVGRVAAVG 72 Query: 72 AGVSEVKMGDRVGVPWLYSACGHCEHCLAAWETVCAH--AEFGGYTKN------GGFAEY 123 A VS+ K+GD VGV + +C C C E C + + G T + GG+++ Sbjct: 73 AHVSKFKVGDVVGVGCIVDSCQSCPDCDEGLENYCDNMVGTYNGKTDDAPGWTMGGYSQQ 132 Query: 124 ILADPRYVAHIPA-GLEAAAAAPIICAGVTSYKGIKECQVQAGDWLAVSGIGGLGHLAVQ 182 I+ RYV +I + AA AP++CAG+T+Y ++ G + V GIGGLGH+ ++ Sbjct: 133 IVVHERYVLNIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHMGIK 192 Query: 183 YGKAMGLRVCAVDIDDGKLAHAQRLGADAVVNARVGDPIARLREVTDGGAHGVLITAP-S 241 AMG V A D K A A+ LGAD VV +R + +A + D + V AP Sbjct: 193 IAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFDFILNTV--AAPHD 250 Query: 242 LEAFHQGVAMTRKRGTCVLVGLPPGEFPTP-LFDVVANCITIRGSFVGTRKDMAEALAFA 300 L+AF +A+ ++ GT LVG P P+P +F+++ ++ GS +G + E L F Sbjct: 251 LDAF---LALLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPETQEMLDFC 307 Query: 301 AAGKVRADFELQPLSAINNVFERLQRGDVPSRVVLD 336 A + AD EL IN +ER+ +GDV R V+D Sbjct: 308 AEHGIVADIELIRADEINTAYERMLKGDVKYRFVID 343 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 146 bits (369), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 119/351 (33%), Positives = 176/351 (50%), Gaps = 40/351 (11%) Query: 12 PLVIKEIPLPSPGAGQILVKTEACGVCHTDLHAAHGDWPVKPSLPFVPGHEGIGVVVAIG 71 PL I+E+ L PG G++LV+ +A G+CH+DL G P +P +P GHE VV +G Sbjct: 22 PLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP-RP-VPMALGHEASAEVVEVG 79 Query: 72 AGVSEVKMGDRVGVPWLYSACGHCEHCLAAWETVCAHAEFGGYTKNGG------------ 119 +GV++++ GD V + ++ S CGHC C+ +C E G T G Sbjct: 80 SGVNDLRAGDLVVLVFVPS-CGHCLPCMEGRPALC---EPGAATNTVGELLSGQRRLRDK 135 Query: 120 ------------FAEYILADPRYVAHIPAGLEAAAAAPIICAGVTSY-KGIKECQVQAGD 166 FA++ + R I A ++ AA CA +T + +V+AG Sbjct: 136 TQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAGT 195 Query: 167 WLAVSGIGGLGHLAVQYGKAMGLR-VCAVDIDDGKLAHAQRLGADAVVNARVGDPIARLR 225 AV G+GG+G A+ A G R V AVD+ D KLA A+ LGA A VNAR D + ++R Sbjct: 196 TAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKVR 255 Query: 226 EVTDGGAHGVLITAPSLEAFHQGVAMTRKRGTCVLVGLP-PGE-FPTPLFDVVANCITIR 283 +T GG A S++A MTR+ G V GLP PG+ + +VA T++ Sbjct: 256 ALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEERTVK 315 Query: 284 GSFVGT---RKDMAEALAFAAAGKVRADF---ELQPLSAINNVFERLQRGD 328 GS++G+ +D+ + AGK+ D E L+ IN F+RL G+ Sbjct: 316 GSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFDRLHGGE 366 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 94.4 bits (233), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 108/374 (28%), Positives = 171/374 (45%), Gaps = 49/374 (13%) Query: 3 AAVVEQFGGPLVIKEIPLPSPGAGQILVKTEACGVCHTDLHAAHGDWPVKPSLPFVPGHE 62 AAV Q G PL I+E+ L P AG++LV+ +A G+CHTD + G P + P + GHE Sbjct: 5 AAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP-EGIFPAILGHE 63 Query: 63 GIGVVVAIGAGVSEVKMGDRVGVPWLYSACGHCEHCLAAWETVC--------------AH 108 G GVVV +G GV +K D V +P C C+ CL+ +C A Sbjct: 64 GAGVVVDVGPGVKSLKKDDHV-IPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDAT 122 Query: 109 AEFG-------GYTKNGGFAEYILADPRYVAHIPAGLEAAAAAPIICAGVTSYKG--IKE 159 + F Y F+ YI+ +A I + I C GVT+ G + Sbjct: 123 SRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGC-GVTTGVGAVLFT 181 Query: 160 CQVQAGDWLAVSGIGGLGHLAVQYGKAMGL-RVCAVDIDDGKLAHAQRLGADAVVNAR-V 217 +V+AG + V G+GG+G +Q K +G ++ VD++ + A A++ G +N + V Sbjct: 182 AKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDV 241 Query: 218 GDPIARLREVTDGGAH-------GVLITAPSLEAFHQGVAMTRKRGTCVLVGLPPG--EF 268 + + + +TDGGA SLE H+G G +++G+ E Sbjct: 242 PNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKG------WGQSIIIGVAAAGQEI 295 Query: 269 PTPLFDVVANCITIRGSFVGT--RKDMAEALAFAAAGKVRADFELQ---PLSAINNVFER 323 T F +V + +F G R D+ + + + GK+ D + PL IN F+ Sbjct: 296 STRPFQLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDL 355 Query: 324 LQRGDVPSRVVLDF 337 ++ G+ R V++F Sbjct: 356 MKSGE-SIRSVVEF 368 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 79.3 bits (194), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 70/234 (29%), Positives = 106/234 (45%), Gaps = 32/234 (13%) Query: 1 MLAAVVEQFGGPLVIK--EIPLPSPGAGQILVKTEACGVCHTDLHAAHGDWPVKPSLPFV 58 M A +++FGGP V++ E P+P AG++L+K A GV D+ G++PV P + Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 Query: 59 PGHEGIGVVVAIGAGVSEVKMGDRVGVPWLYSACGHCEHCLAAWETVCAHAEFGGYTKNG 118 PG E G +VA G S K+GD VCA + G Sbjct: 61 PGLEVAGEIVAGDLGDSGFKLGD----------------------LVCA------LVQGG 92 Query: 119 GFAEYILADPRYVAHIPAGLEAAAAAPIICAGVTSYKGIKECQVQAGD--WLAVSGIGGL 176 G+AE+ A +P GL A AA + T + + + +G+ L G G+ Sbjct: 93 GYAEFCAAPLAQCLPVPKGLSALEAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGI 152 Query: 177 GHLAVQYGKAMGLRVCAVDIDDGKLAHAQRLGADAVVNARVGDPIARLREVTDG 230 G A+Q +A+G RV A K + LGA+ +N + D A ++E+T G Sbjct: 153 GVTAIQIARALGHRVFATAGSAEKCRACEELGAERAINYKTEDFEAVVKELTGG 206 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,114,676 Number of extensions: 46584 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 10 Length of query: 338 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 252 Effective length of database: 1,212,660 Effective search space: 305590320 Effective search space used: 305590320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)