BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0228.p (595 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synt... 595 e-171 gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthe... 344 1e-95 >gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synthase [Herbaspirillum seropedicae SmR1] Length = 590 Score = 595 bits (1535), Expect = e-171, Method: Compositional matrix adjust. Identities = 299/563 (53%), Positives = 376/563 (66%), Gaps = 4/563 (0%) Query: 36 RLDLMSNAWLGRLTGNISPAALLNAYVDWLTHLALSPTKRAALMQTAWQNWWRMYAYSRS 95 RLD +A L R +G++S + L A DW HLA+SP KR L+ A Q + Y Sbjct: 27 RLDRQIHAALARASGSLSLVSGLLAVTDWAAHLAVSPGKRLELLCLALQQANHLLHYISR 86 Query: 96 CQLGNEQACIEPLPQDRRFSDPAWQQWPFNTIHQAFLLQQQWWHRATTGVRGQSQHHEDV 155 + + DRRFS+P WQ+WPFN HQ+FLL +QWW AT V G +HH D+ Sbjct: 87 SSSSDPDSPSMLPVADRRFSNPDWQRWPFNLWHQSFLLAEQWWAAATRDVWGVEKHHADL 146 Query: 156 VTFTVRQILDMLAPSNFLSTNPVAQHATLASGGGNLAQGWLNAMSDWRASIAPGEEAQPG 215 V F RQ LD+ +PSN L TNP T+ G NL G N M+D + + Sbjct: 147 VAFAARQCLDVFSPSNQLLTNPEVLRITMEQRGANLLMGMQNCMNDMIGLLLEKPASTAR 206 Query: 216 AFKVGKDVAITPGKVVFHNDLIELIQYAPSTKTTHAVPLLFVPAWIMKYYILDLSPRNSL 275 F G+DVA++PGKVV N ++ELI+YAP+T+T PLL +PAWIMKYYILDLSP NSL Sbjct: 207 TFMPGRDVAVSPGKVVLRNRIMELIRYAPATETVCPEPLLLIPAWIMKYYILDLSPANSL 266 Query: 276 VKYLVDLGYTVFMISWKNPQKEDRELSMEDYRRLGVMAAIDAVTAQTGAKRINATGYCLG 335 ++YLV+ G+TVF +SWKNP DR+LSM+DY G AA+DAV + ++++ GYCLG Sbjct: 267 IRYLVEKGHTVFCVSWKNPAPADRDLSMDDYLDSGFHAALDAVNSACPDRKVHGVGYCLG 326 Query: 336 GTLLSIAAATMARDGDDRLASMSLFASQVDFEEPGELSLFIDESQVSFLEAVMWEQGYLD 395 GTLLSIAAA MARDGD RLAS++L A+Q DF EPGEL LFIDESQ+S LEA M + G L Sbjct: 327 GTLLSIAAAAMARDGDQRLASLTLLAAQTDFTEPGELGLFIDESQISLLEAQMQQAGTLS 386 Query: 396 TKQMAGAFQLLRSNDLIWSRRLKQYLLGLPEEKSDLMAWNADATRMPYRMHSEYLRQLFL 455 QMA AFQ+LRS DL+WSR ++QYL+G+P SDLM WNADATRMP MHS+YLR+LFL Sbjct: 387 QSQMAAAFQMLRSYDLLWSRVIRQYLMGVPAHNSDLMEWNADATRMPALMHSQYLRRLFL 446 Query: 456 HNDLAEGRYCTEGKPISLSDIRLPVFAVGTVTDHVAPWRSAFKIHRLTDTDVTFVLTSGG 515 +DL+EGRY +GKP+ L+D+ LPVF VGTV DHVAPWRS +K+H L ++TFVL SGG Sbjct: 447 DDDLSEGRYEVDGKPVLLADVNLPVFLVGTVRDHVAPWRSVYKLHSLCPAEITFVLASGG 506 Query: 516 HNAGVVSPPGHPHRSYQIATHADKQGMVDADTWQAGVALHDGSWWPAWEAWLRKHSGKRT 575 HNAG+VSPPGHP +QI+T A AD W AG ++ GSWWPAW WL + SG Sbjct: 507 HNAGIVSPPGHPRSQFQISTRAAGASYRSADEWMAGTSVTTGSWWPAWADWLAQRSGTPV 566 Query: 576 APPAL---PAHRPA-APGKYVLQ 594 + AH A APG Y+++ Sbjct: 567 PAAGMGYDKAHSLADAPGNYIME 589 >gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 588 Score = 344 bits (883), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 196/502 (39%), Positives = 283/502 (56%), Gaps = 18/502 (3%) Query: 103 ACIEPLPQDRRFSDPAWQQWPFNTIHQA-FLLQQQWWHRATTGVRGQSQHHEDVVTFTVR 161 A P DRR S PAWQ+ P+ A + L ++ V+G S+ + F V+ Sbjct: 84 AAKTPAITDRRLSAPAWQEHPYFAFQAAAYQLNSRFLLALADAVQG-SEKVRHKLRFAVQ 142 Query: 162 QILDMLAPSNFLSTNPVAQHATLASGGGNLAQGWLNAMSDW-RASIAPGEEAQPGAFKVG 220 Q++D ++P+NFL+TNPVAQ L + G +L +G ++D + ++ +E AF+VG Sbjct: 143 QMIDAMSPANFLATNPVAQQKILETRGESLTKGIAQLLADLQKGHVSQTDET---AFEVG 199 Query: 221 KDVAITPGKVVFHNDLIELIQYAPSTKTTHAVPLLFVPAWIMKYYILDLSPRNSLVKYLV 280 +DV + G V+F N+L +LIQY P TKT H VPLL VP I K+YILDL P NSLV+Y V Sbjct: 200 RDVGASEGSVIFENELFQLIQYKPLTKTVHEVPLLIVPPCINKFYILDLQPHNSLVRYTV 259 Query: 281 DLGYTVFMISWKNPQKEDRELSMEDYRRLGVMAAIDAVTAQTGAKRINATGYCLGGTLLS 340 + G+TVF+ISW+N + + Y V+ +I V + +INA G+C+GGT+LS Sbjct: 260 EQGHTVFLISWRNADAAVETATWDQYVDDAVVRSIHIVQEISKQPQINALGFCVGGTILS 319 Query: 341 IAAATMARDGDDRLASMSLFASQVDFEEPGELSLFIDESQVSFLEAVMWEQGYLDTKQMA 400 A A +A G+D +AS++L + +DF + G + ++IDE Q++ E ++ ++G + + A Sbjct: 320 CALALLAARGEDPVASLTLLTTFLDFSDTGAIDVYIDEMQLALREQMIGQRGLMAGRDFA 379 Query: 401 GAFQLLRSNDLIWSRRLKQYLLGLPEEKSDLMAWNADATRMPYRMHSEYLRQLFLHNDLA 460 AF LR NDL+W+ YL G DL+ WNAD+T +P M YLR ++L N L Sbjct: 380 SAFSSLRPNDLLWNYVESNYLKGEKPSAFDLLYWNADSTNLPGPMFCYYLRHMYLENALK 439 Query: 461 E-GRYCTEGKPISLSDIRLPVFAVGTVTDHVAPWRSAFKIHRLTDT----DVTFVLTSGG 515 E GR G+ I L I P F + DH+ PW+SA+ L +T + FVL + G Sbjct: 440 EPGRLTVGGEKIDLRKINAPAFIYASRDDHIVPWKSAYASLGLINTAKRSNNRFVLGASG 499 Query: 516 HNAGVVSPPGHPHRSYQIATHADKQGMVDADTWQAGVALHDGSWWPAWEAWLRKHSGKRT 575 H AGV++P R+Y I A + AD W AG H GSWW W A+L KH G++ Sbjct: 500 HIAGVINPASKNKRNYWINDKA--KAGSGADAWLAGATEHPGSWWGQWAAFLAKHGGRKV 557 Query: 576 APPALPA-----HRPAAPGKYV 592 A P H APG+YV Sbjct: 558 AAPKAAGSTRYKHIEPAPGRYV 579 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,024,664 Number of extensions: 88327 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 2 Length of query: 595 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 504 Effective length of database: 1,188,985 Effective search space: 599248440 Effective search space used: 599248440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)